外施乙烯对钩藤生理及主要药用成分积累的影响
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摘要
以4年生钩藤为实验材料,研究了不同浓度的乙烯对其生理以及主要药用成分(钩藤碱和异钩藤碱)积累的影响。结果表明:外施乙烯能抑制钩藤净光合速率、诱导气孔关闭、降低蒸腾速率、提高胞间CO2浓度、显著提高叶中脯氨酸含量,但对叶中可溶性糖、丙二醛及叶绿素含量影响较小;同时,乙烯对钩藤生理的影响与处理时间有关,双因素方差F值显示,导致叶中脯氨酸、可溶性糖以及丙二醛积累的主导因素均为处理时间。乙烯对主要药用成分积累的调控表现出二重性, 50和500 mg·L~(-1)处理促进叶片和带钩茎枝中主要药用成分的积累,而浓度为2 000 mg·L~(-1)时,则表现为抑制积累;调控还呈现器官差异性, 1 000 mg·L~(-1)处理抑制叶片主要药用成分的积累,但促进其在带钩茎枝中的积累;导致叶中主要药用成分变化的主导因素是浓度,而在带钩茎枝中主导因素为处理时间。此外,乙烯的调控作用,能让同一部位的钩藤碱和异钩藤碱含量发生同步变化。
To study the effect of ethylene with different concentrations on physiology and accumulation of main medicinal components(rhynchophylline and isorhynchophylline), four-year-old Uncaria rhynchophylla was carried out as the experimental materials. The results showed that ethylene inhibited net photosynthetic rate, induced stomatal closure, decreased transpiration rate, increased intercellular CO2 concentration, especially it increased the proline content in Uncaria rhynchophylla leaves significantly, but had little effect on soluble sugar, malondialdehyde and chlorophyll content. Moreover, the physiological effect was related to treatment time. F value of two-factor variance analysis indicated treatment time was the main factor which caused the accumulation of proline, soluble sugar and malondialdehyde in leaves. The regulation of ethylene on the accumulation of main medicinal components showed duality. The accumulation of main medicinal ingredients in leaves and branches with hooks was promoted by 50 and 500 mg·L~(-1) treatments, but inhibited by 2 000 mg·L~(-1). This regulation also showed organ differences. 1 000 mg·L~(-1) treatment inhibited the accumulation of main medicinal components in leaves, but promoted their accumulation in branches with hooks. The concentration was the dominant factor leading to the change of the main medicinal components in leaves, while the treatment time was the dominant factor in the branches with hooks. In addition, the regulation of ethylene can change the contents of rhynchophylline and isorhynchophylline in the same part synchronously.
To study the effect of ethylene with different concentrations on physiology and accumulation of main medicinal components(rhynchophylline and isorhynchophylline), four-year-old Uncaria rhynchophylla was carried out as the experimental materials. The results showed that ethylene inhibited net photosynthetic rate, induced stomatal closure, decreased transpiration rate, increased intercellular CO2 concentration, especially it increased the proline content in Uncaria rhynchophylla leaves significantly, but had little effect on soluble sugar, malondialdehyde and chlorophyll content. Moreover, the physiological effect was related to treatment time. F value of two-factor variance analysis indicated treatment time was the main factor which caused the accumulation of proline, soluble sugar and malondialdehyde in leaves. The regulation of ethylene on the accumulation of main medicinal components showed duality. The accumulation of main medicinal ingredients in leaves and branches with hooks was promoted by 50 and 500 mg·L~(-1) treatments, but inhibited by 2 000 mg·L~(-1). This regulation also showed organ differences. 1 000 mg·L~(-1) treatment inhibited the accumulation of main medicinal components in leaves, but promoted their accumulation in branches with hooks. The concentration was the dominant factor leading to the change of the main medicinal components in leaves, while the treatment time was the dominant factor in the branches with hooks. In addition, the regulation of ethylene can change the contents of rhynchophylline and isorhynchophylline in the same part synchronously.
引文
Cao WH,Liu J,He XJ,et al(2007).Modulation of ethylene responses affects plant salt-stress responses.Plant Physiol,143:707-719
Chang BW,Liu J,Zhong P,et al(2018).Effects of exogenous ethylene on physiology and alkaloid accumulations in Catharanthus roseus.Bull Bot Res,38:284-291(in Chinese with English abstract)[常博文,刘杰,钟鹏等(2018).外源乙烯对长春花生理水平和生物碱积累的影响.植物研究,38:284-291]
Cheng Y,Guan J(2014).Involvement of pheophytinase in ethylene-mediated chlorophyll degradation in the peel of harvested‘Yali’pear.J Plant Growth Regul,33:364-372
Desikan R,Last K,Harrett-Williams R,et al(2006).Ethylene-induced stomatal closure in Arabidopsis occurs via Atrboh F-mediated hydrogen peroxide synthesis.Plant J,47:907-916
El-Maarouf-Bouteau H,Sajjad Y,Bazin J,et al(2014).Reactive oxygen species,abscisic acid and ethylene interact to regulate sunflower seed germination.Plant Cell Environ,38:364-374
Flora of China Editorial Committee of CAS(1999).Flora of China.Beijing:Science Press,71:255(in Chinese)[中国科学院《中国植物志》编委会(1999).中国植物志.北京:科学出版社,71:255]
Guo LJ,Du JP,Wu YP,et al(2017).Photosynthetic characteristics of Emmenopterys henryi seedlings and their relationships with environmental factors.Chin J Appl Ecol,28:1473-1481(in Chinese with English abstract)[郭连金,杜佳朋,吴艳萍等(2017).香果树实生苗的光合特性及其与环境因子的关系.应用生态学报,28:1473-1481]
Guo Q,Ma X,Wei S,et al(2014).De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla,a non-model plant with potent anti-alzheimer’s properties.BMC Genomics,15:676
He J,Yue X,Wang R,et al(2011).Ethylene mediates UV-B-induced stomatal closure via peroxidase-dependent hydrogen peroxide synthesis in Vicia faba L.J Exp Bot,62:2657-2666
Huang J,Chen ZF,Yin LY,et al(2018).Effects of three plant exogenous hormones on the biomass,chlorophyll fluorescence parameters,and photosynthetic characteristics of Tabebuia chrysantha seedlings.Plant Sci J,36:745-754(in Chinese with English abstract)[黄杰,陈宗福,尹丽英等(2018).三种外源植物激素对黄花风铃木幼苗生物量、叶绿素荧光参数及光合特性的影响.植物科学学报,36:745-754]
Huang Y,Zhou WX,Zhang YX,et al(2018).Research progresses in pharmacological effects of rhynchophylline and its isomers on nervous system.Acta Pharm Sin,53:1017-1022(in Chinese with English abstract)[黄晏,周文霞,张永祥等(2018).钩藤碱及其同分异构体对神经系统作用的研究进展.药学学报,53:1017-1022]
Kageyama A,Ishizaki K,Kohchi T,et al(2015).Abscisic acid induces biosynthesis of bisbibenzyls and tolerance to UV-C in the liverwort Marchantia polymorpha.Phytochemistry,117:547-553
Kim KJ,Park CJ,Ham BK,et al(2006).Induction of a cytosolic pyruvate kinase 1 gene during the resistance response to Tobacco mosaic virus in Capsicum annuum.Plant Cell Rep,25:359-364
Krasensky J,Jonak C(2012).Drought,salt,and temperature stress-induced metabolic rearrangements and regulatory networks.J Exp Bot,63:1593-1608
Lee Y,Lee DE,Lee HS,et al(2011).Influence of auxins,cytokinins,and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree(Morus alba L.).Plant Cell Tiss Org,105:9-19
Li HS(2000).Principle and Technology of Plant Physiological and Biochemical Experiments.Beijing:Higher Education Press(in Chinese)[李合生(2000).植物生理生化实验原理和技术.北京:高等教育出版社]
Li L,Xiao LT(2013).Handbook for the Application of Plant Growth Regulators.Beijing:Chemical Industry Press,32-33(in Chinese)[李玲,肖浪涛(2013).植物生长调节剂应用手册.北京:化学工业出版社,32-33]
Liu X,Huang B(2000).Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass.Crop Sci,40:503-510
National Pharmacopoeia Commission(2015).Pharmacopoeia of the People’s Republic of China.Beijing:China Medical Science and Technology Press(in Chinese)[国家药典委员会(2015).中华人民共和国药典.北京:中国医药科技出版社]
Ndagijimana A,Wang X,Pan G,et al(2013).A review on indole alkaloids isolated from Uncaria rhynchophylla and their pharmacological studies.Fitoterapia,86:35-47
Shibli RA,Smith MAL,Kushad M(1997).Headspace ethylene accumulation effects on secondary metabolite production in Vaccinium pahalae cell culture.Plant Growth Regul,23:201-205
Su WH,Zhang GF,Li XH,et al(2005).Relationship between accumulation of secondary metabolism in medicinal plant and environmental condition.Chin Trad Herb Drugs,36:1415-1418(in Chinese with English abstract)[苏文华,张光飞,李秀华等(2005).植物药材次生代谢产物的积累与环境的关系.中草药,36:1415-1418]
Wang H,Wang F,Zheng F,et al(2016).Ethylene-insensitive mutants of Nicotiana tabacum exhibit drought stress resistance.Plant Growth Regul,79:107-117
Wu SW,Yang MQ,Xiao YL(2018).Synthetic biology studies of monoterpene indole alkaloids.Chin J Org Chem,38:2243-2258(in Chinese with English abstract)[吴世文,杨盟权,肖友利(2018).单萜吲哚生物碱的合成生物学研究进展.有机化学,38:2243-2258]
Xu JY,Wang XH,Yang SW,et al(2018).Optimization on extracting conditions of rhynchophylline and isorhynchophylline in Uncaria rhynchophylla leaves by response surface method.J Cent South Univ For Technol,38:131-138(in Chinese with English abstract)[徐佳瑜,王晓红,杨胜伟等(2018).响应曲面法优化钩藤叶中钩藤碱和异钩藤碱的提取条件.中南林业科技大学学报,38:131-138]
Yang XM,Wu XL,Liu YF,et al(2018).Analysis of chlorophyll and photosynthesis of a tomato chlorophyll-deficient mutant induced by EMS.Chin J Appl Ecol,29:1983-1989(in Chinese with English abstract)[杨小苗,吴新亮,刘玉凤等(2018).一个番茄EMS叶色黄化突变体的叶绿素含量及光合作.应用生态学报,29:1983-1989]
Zhang JG,Chen JJ,Geng CA(2019).Advances in indole alkaloids from traditional Chinese medicine of Uncariae Ramulus Cum Uncis documented in Chinese Pharmacopoeia.Chin J Chin Mater Med,44:1-14(in Chinese with English abstract)[张建钢,陈纪军,耿长安(2019).《中国药典》收载的钩藤基原植物吲哚生物碱研究进展.中国中药杂志,44:1-14]
Zhang ZM,Song WW,Ding H,et al(2013).The responses of leaf osmoregulation substance and protective enzyme activity of different peanut cultivars to non-sufficient irrigation.Acta Ecol Sin,33:4257-4265(in Chinese with English abstract)[张智猛,宋文武,丁红等(2013).不同生育期花生渗透调节物质含量和抗氧化酶活性对土壤水分的响应.生态学报,33:4257-4265]
Zhu SM,Song HL,Zhang L,et al(2018).Effect of lead stress on growth and physio-biochemical indices of tobacco.Plant Physiol J,54:465-472(in Chinese with English abstract)[朱诗苗,宋杭霖,张丽等(2018).铅胁迫对烟草生长及生理生化指标的影响.植物生理学报,54:465-472]
Chang BW,Liu J,Zhong P,et al(2018).Effects of exogenous ethylene on physiology and alkaloid accumulations in Catharanthus roseus.Bull Bot Res,38:284-291(in Chinese with English abstract)[常博文,刘杰,钟鹏等(2018).外源乙烯对长春花生理水平和生物碱积累的影响.植物研究,38:284-291]
Cheng Y,Guan J(2014).Involvement of pheophytinase in ethylene-mediated chlorophyll degradation in the peel of harvested‘Yali’pear.J Plant Growth Regul,33:364-372
Desikan R,Last K,Harrett-Williams R,et al(2006).Ethylene-induced stomatal closure in Arabidopsis occurs via Atrboh F-mediated hydrogen peroxide synthesis.Plant J,47:907-916
El-Maarouf-Bouteau H,Sajjad Y,Bazin J,et al(2014).Reactive oxygen species,abscisic acid and ethylene interact to regulate sunflower seed germination.Plant Cell Environ,38:364-374
Flora of China Editorial Committee of CAS(1999).Flora of China.Beijing:Science Press,71:255(in Chinese)[中国科学院《中国植物志》编委会(1999).中国植物志.北京:科学出版社,71:255]
Guo LJ,Du JP,Wu YP,et al(2017).Photosynthetic characteristics of Emmenopterys henryi seedlings and their relationships with environmental factors.Chin J Appl Ecol,28:1473-1481(in Chinese with English abstract)[郭连金,杜佳朋,吴艳萍等(2017).香果树实生苗的光合特性及其与环境因子的关系.应用生态学报,28:1473-1481]
Guo Q,Ma X,Wei S,et al(2014).De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla,a non-model plant with potent anti-alzheimer’s properties.BMC Genomics,15:676
He J,Yue X,Wang R,et al(2011).Ethylene mediates UV-B-induced stomatal closure via peroxidase-dependent hydrogen peroxide synthesis in Vicia faba L.J Exp Bot,62:2657-2666
Huang J,Chen ZF,Yin LY,et al(2018).Effects of three plant exogenous hormones on the biomass,chlorophyll fluorescence parameters,and photosynthetic characteristics of Tabebuia chrysantha seedlings.Plant Sci J,36:745-754(in Chinese with English abstract)[黄杰,陈宗福,尹丽英等(2018).三种外源植物激素对黄花风铃木幼苗生物量、叶绿素荧光参数及光合特性的影响.植物科学学报,36:745-754]
Huang Y,Zhou WX,Zhang YX,et al(2018).Research progresses in pharmacological effects of rhynchophylline and its isomers on nervous system.Acta Pharm Sin,53:1017-1022(in Chinese with English abstract)[黄晏,周文霞,张永祥等(2018).钩藤碱及其同分异构体对神经系统作用的研究进展.药学学报,53:1017-1022]
Kageyama A,Ishizaki K,Kohchi T,et al(2015).Abscisic acid induces biosynthesis of bisbibenzyls and tolerance to UV-C in the liverwort Marchantia polymorpha.Phytochemistry,117:547-553
Kim KJ,Park CJ,Ham BK,et al(2006).Induction of a cytosolic pyruvate kinase 1 gene during the resistance response to Tobacco mosaic virus in Capsicum annuum.Plant Cell Rep,25:359-364
Krasensky J,Jonak C(2012).Drought,salt,and temperature stress-induced metabolic rearrangements and regulatory networks.J Exp Bot,63:1593-1608
Lee Y,Lee DE,Lee HS,et al(2011).Influence of auxins,cytokinins,and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree(Morus alba L.).Plant Cell Tiss Org,105:9-19
Li HS(2000).Principle and Technology of Plant Physiological and Biochemical Experiments.Beijing:Higher Education Press(in Chinese)[李合生(2000).植物生理生化实验原理和技术.北京:高等教育出版社]
Li L,Xiao LT(2013).Handbook for the Application of Plant Growth Regulators.Beijing:Chemical Industry Press,32-33(in Chinese)[李玲,肖浪涛(2013).植物生长调节剂应用手册.北京:化学工业出版社,32-33]
Liu X,Huang B(2000).Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass.Crop Sci,40:503-510
National Pharmacopoeia Commission(2015).Pharmacopoeia of the People’s Republic of China.Beijing:China Medical Science and Technology Press(in Chinese)[国家药典委员会(2015).中华人民共和国药典.北京:中国医药科技出版社]
Ndagijimana A,Wang X,Pan G,et al(2013).A review on indole alkaloids isolated from Uncaria rhynchophylla and their pharmacological studies.Fitoterapia,86:35-47
Shibli RA,Smith MAL,Kushad M(1997).Headspace ethylene accumulation effects on secondary metabolite production in Vaccinium pahalae cell culture.Plant Growth Regul,23:201-205
Su WH,Zhang GF,Li XH,et al(2005).Relationship between accumulation of secondary metabolism in medicinal plant and environmental condition.Chin Trad Herb Drugs,36:1415-1418(in Chinese with English abstract)[苏文华,张光飞,李秀华等(2005).植物药材次生代谢产物的积累与环境的关系.中草药,36:1415-1418]
Wang H,Wang F,Zheng F,et al(2016).Ethylene-insensitive mutants of Nicotiana tabacum exhibit drought stress resistance.Plant Growth Regul,79:107-117
Wu SW,Yang MQ,Xiao YL(2018).Synthetic biology studies of monoterpene indole alkaloids.Chin J Org Chem,38:2243-2258(in Chinese with English abstract)[吴世文,杨盟权,肖友利(2018).单萜吲哚生物碱的合成生物学研究进展.有机化学,38:2243-2258]
Xu JY,Wang XH,Yang SW,et al(2018).Optimization on extracting conditions of rhynchophylline and isorhynchophylline in Uncaria rhynchophylla leaves by response surface method.J Cent South Univ For Technol,38:131-138(in Chinese with English abstract)[徐佳瑜,王晓红,杨胜伟等(2018).响应曲面法优化钩藤叶中钩藤碱和异钩藤碱的提取条件.中南林业科技大学学报,38:131-138]
Yang XM,Wu XL,Liu YF,et al(2018).Analysis of chlorophyll and photosynthesis of a tomato chlorophyll-deficient mutant induced by EMS.Chin J Appl Ecol,29:1983-1989(in Chinese with English abstract)[杨小苗,吴新亮,刘玉凤等(2018).一个番茄EMS叶色黄化突变体的叶绿素含量及光合作.应用生态学报,29:1983-1989]
Zhang JG,Chen JJ,Geng CA(2019).Advances in indole alkaloids from traditional Chinese medicine of Uncariae Ramulus Cum Uncis documented in Chinese Pharmacopoeia.Chin J Chin Mater Med,44:1-14(in Chinese with English abstract)[张建钢,陈纪军,耿长安(2019).《中国药典》收载的钩藤基原植物吲哚生物碱研究进展.中国中药杂志,44:1-14]
Zhang ZM,Song WW,Ding H,et al(2013).The responses of leaf osmoregulation substance and protective enzyme activity of different peanut cultivars to non-sufficient irrigation.Acta Ecol Sin,33:4257-4265(in Chinese with English abstract)[张智猛,宋文武,丁红等(2013).不同生育期花生渗透调节物质含量和抗氧化酶活性对土壤水分的响应.生态学报,33:4257-4265]
Zhu SM,Song HL,Zhang L,et al(2018).Effect of lead stress on growth and physio-biochemical indices of tobacco.Plant Physiol J,54:465-472(in Chinese with English abstract)[朱诗苗,宋杭霖,张丽等(2018).铅胁迫对烟草生长及生理生化指标的影响.植物生理学报,54:465-472]