HPLC法测定新疆软紫草属4种植物根中萘醌类成分
摘要
目的采用HPLC法测定新疆软紫草属4种植物(新疆紫草、黄花软紫草、天山软紫草、硬萼软紫草)根中7种萘醌类成分(左旋紫草素、乙酰紫草素、β-乙酰氧基异戊酰阿卡宁、去氧紫草素、异丁酰紫草素、β,β′-二甲基丙烯酰阿卡宁、2-甲基丁基酰紫草素)的含有量。方法该药材石油醚提取物的分析采用Agilent-C_(18)柱(250 mm×4.6 mm,5μm);流动相乙腈-0.05%甲酸;体积流量1 mL/min;柱温30℃;检测波长275 nm。结果 7种成分在各自范围内线性关系良好,平均加样回收率为97.37%～99.75%,RSD为0.42%～1.94%。结论该方法准确稳定,重复性好,可用于软紫草的质量控制。
引文
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[18] 徐海燕, 丁文欢, 张慧, 等. 薄层扫描法测定新疆不同产地新疆紫草中β,β′-二甲基丙烯酰阿卡宁的含有量[J]. 新疆医科大学学报, 2017, 40(5): 642-645.
[19] 曾祖平, 韩旭阳, 王宏, 等. HPLC法测定紫草中萘醌类成分[J]. 解放军药学学报, 2013, 29(5): 453-455.
[20] 孟庆举, 易红, 杨华, 等. 闪式提取法用于紫草的工艺条件研究[J]. 中国中药杂志, 2013, 38(14): 2302-2305.
[21] 詹志来, 胡峻, 刘谈, 等. 紫草化学成分与药理活性研究进展[J]. 中国中药杂志, 2015, 40(21): 4127-4135.
[22] 米吉提·胡达拜尔地, 潘晓玲. 新疆植物志[M]. 乌鲁木齐: 新疆科技出版社, 2004: 150-156.
[23] 昝珂, 苏蕊, 滕爱君, 等. 新疆紫草HPLC特征图谱和紫草类药材6种萘醌类成分含有量测定[J].药物分析杂志, 2016, 36(9): 1526-1535.
[2] 夏纯, 戴明, 游冬阁, 等. 复方紫草油临床应用探讨[J]. 世界中医药, 2016, 11(9): 1895-1896,1900.
[3] Zhao H, Chang Q S, Qi D L, et al. Overexpression of LeMYB1 enhances shikonin formation by up-regulating key shikonin biosynthesis-related genes in Lithospermum erythrorhizon[J]. Biol Plant, 2015, 59(3): 429-435.
[4] Zhao X R, Zhou Y L, Wang L Y, et al. Shikonin alleviates the biotoxicity produced by pneumococcal pneumolysin[J]. Life Sci, 2017, 177(5): 1-7.
[5] 杨阳,陶仕英,牛建昭, 等.紫草素对宫颈癌SiHa细胞增殖周期的影响[J].环球中医药, 2018, 11(1): 6-10.
[6] 行敏,任宏伟,屈慧明, 等.寻常型银屑病患者血清中IL-17的表达及紫草素对IL-17刺激HaCaT细胞分泌IL-6和IL-23的影响[J]. 吉林大学学报(医学版), 2017, 43(5): 958-962.
[7] 马阿西叶,周建业,施文, 等.紫草素和胡桃醌对舌癌Tca8113细胞的增殖抑制及凋亡诱导作用比较研究[J].中国实用口腔科杂志, 2017, 10(9): 550-553.
[8] Huang C S, Chen H W, Lin T Y, et al. Shikonin upregulates the expression of drug-metabolizing enzymes and drug transporters in primary rat hepatocytes[J].J Ethnopharmacol, 2018, 216(4): 18-25.
[9] Li M Y, Mi C L, Wang K S, et al. Shikonin suppresses proliferation and induces cell cycle arrest through the inhibition of hypoxia-inducible factor-1α signaling[J]. Chem-Biol Interact, 2017, 274(8): 58-67.
[10] Tong Y N, Chuan J L, Bai L, et al. The protective effect of shikonin on renal tubular epithelial cell injury induced by high glucose[J]. Biomed Pharmacother, 2018, 98(2): 701-708.
[11] Tang S W, Chen A, Zhou X J, et al. Assessment of the inhibition risk of shikonin on cytochrome P450 via cocktail inhibition assay[J]. Toxicol Lett, 2017, 281(11): 74-83.
[12] Fujita Y,Maeda Y, Suga C, et al. Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon. Ⅲ. Comparison of shikonin derivatives of cultured cells and ko-shikon[J]. Plant Cell Rep, 1983, 2(4): 192-193.
[13] Albreht A, Vovk I, Simonovsk B, et al. Identification of shikonin and its ester derivatives from the roots of Echium italicum L.[J]. J Chromatogr A, 2009, 1216(15): 3156-3162.
[14] Gao Y S, Wu L P, Zhang K X, et al. Electroanalytical method for determination of shikonin based on the enhancement effect of cyclodextrin functionalized carbon nanotubes[J]. Chin Chem Lett, 2015, 26(5): 613-618.
[15] Wu L P, Xu J K, Lu L M, et al. Fabrication of nanostructured PEDOT clusters using β-cyclodextrin as substrate and applied for simultaneous determination of hyperoside and shikonin[J]. Colloid Surface A, 2015, 482(10): 203-212
[16] 张蕾,张爱芹,王嫚, 等.新疆紫草的毛细管电泳指纹图谱研究[J].分析化学, 2017, 45(11): 1727-1733.
[17] 范芳芳, 刘泽蒲, 陈文, 等.紫外分光光度法测定新疆紫草中总萘醌的含有量[J]. 石河子大学学报(自然科学版), 2004, 22(5): 425-426.
[18] 徐海燕, 丁文欢, 张慧, 等. 薄层扫描法测定新疆不同产地新疆紫草中β,β′-二甲基丙烯酰阿卡宁的含有量[J]. 新疆医科大学学报, 2017, 40(5): 642-645.
[19] 曾祖平, 韩旭阳, 王宏, 等. HPLC法测定紫草中萘醌类成分[J]. 解放军药学学报, 2013, 29(5): 453-455.
[20] 孟庆举, 易红, 杨华, 等. 闪式提取法用于紫草的工艺条件研究[J]. 中国中药杂志, 2013, 38(14): 2302-2305.
[21] 詹志来, 胡峻, 刘谈, 等. 紫草化学成分与药理活性研究进展[J]. 中国中药杂志, 2015, 40(21): 4127-4135.
[22] 米吉提·胡达拜尔地, 潘晓玲. 新疆植物志[M]. 乌鲁木齐: 新疆科技出版社, 2004: 150-156.
[23] 昝珂, 苏蕊, 滕爱君, 等. 新疆紫草HPLC特征图谱和紫草类药材6种萘醌类成分含有量测定[J].药物分析杂志, 2016, 36(9): 1526-1535.
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