甲基磺酸乙酯对不同基因型加工番茄诱变效应
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摘要
采用甲基磺酸乙酯(ethyl methylsulfone, EMS)溶液浸种处理3种基因型加工番茄材料的种子12 h,通过种子萌发期和苗期相关指标测定,确定种子诱变的合适浓度。结果表明,EMS试剂对种子的萌发有抑制和致死作用,且浓度越高影响越大。不同基因型加工番茄种子对EMS的耐受程度不同,JW001的半致死剂量为2.5%EMS处理12 h,JW9的半致死剂量为3.0%EMS处理12 h,里格尔87-5的半致死剂量为2.0%EMS处理12 h。探明EMS对不同基因型加工番茄种子萌发的影响,以期为不同基因型加工番茄的化学诱变效应提供依据。进一步为创新加工番茄种质资源、培育加工番茄新品种提供参考。
Seeds of three genotypes processed tomato materials were soaked with ethyl methanesulfonate(EMS)solution for 12 h. The appropriate concentration of seed mutagenesis was determined by seed germination and seedling stage indicators. The results showed that EMS reagent had inhibitory and lethal effects on seed germination and the higher the concentration, the greater the effect. The tolerance of different genotypes of tomato seeds to EMS was different. The semi-lethal dose of JW001 was 2.5% EMS for 12 h, the semi-lethal dose of JW9 was 3.0%EMS for 12 h, and the semi-lethal dose of Rieger 87-5 was 2.0% EMS processing 12 h. The effects of EMS on seed germination of different genotypes of processed tomato were studied in order to provide basis for chemical mutagenesis of different genotypes of processed tomato. And further provides a reference for the innovative processing of the tomato germplasm resources and the cultivation of a new tomato variety.
Seeds of three genotypes processed tomato materials were soaked with ethyl methanesulfonate(EMS)solution for 12 h. The appropriate concentration of seed mutagenesis was determined by seed germination and seedling stage indicators. The results showed that EMS reagent had inhibitory and lethal effects on seed germination and the higher the concentration, the greater the effect. The tolerance of different genotypes of tomato seeds to EMS was different. The semi-lethal dose of JW001 was 2.5% EMS for 12 h, the semi-lethal dose of JW9 was 3.0%EMS for 12 h, and the semi-lethal dose of Rieger 87-5 was 2.0% EMS processing 12 h. The effects of EMS on seed germination of different genotypes of processed tomato were studied in order to provide basis for chemical mutagenesis of different genotypes of processed tomato. And further provides a reference for the innovative processing of the tomato germplasm resources and the cultivation of a new tomato variety.
引文
Cui X.,Liang Y.,and Wang H.L.,2013,Effects of different concentrations of chemical mutagen EMS on tomato seeds germination,Beifang Yuanyi(Northern Horticulture),(14):28-30(崔霞,梁燕,王玲慧,2013,不同浓度化学诱变剂甲基磺酸乙酯对番茄种子发芽的影响,北方园艺,(14):28-30)
Han J.,2005,Studies on creation technique of germplasm resources in roses,Thesis M.S.,Shandong Agricultural University,Supervisor:Feng Z.,pp.4-7(韩进,2005,月季种质资源创新技术的研究,硕士学位论文,山东农业大学,导师:丰震,pp.4-7)
He H.Y.,Fan L.N.,Qi Y.W.,Lao F.Y.,Deng H.H.,and Hu F.,2016,Muta genesis application to Saccharum officinarum shoot tip culture with EMS,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),32(6):55-60(何慧怡,樊丽娜,齐永文,劳方业,邓海华,胡斐,2016,EMS诱变甘蔗茎尖分生组织的初步研究,中国农学通报,32(6):55-60)
Heigwer F.,Kerr G.,and Boutros M.,2014,E-CRISP:fastCRISPRtarget site identification,Nat.Methods,11(2):122-123
Liu G.Z.,Wang Y.F.,Meng X.F.,and Ye Z.B.,2017,Phenotype mutation and ascorbate characterization in M2population derived from EMS treatment in'Ligeer 87-5'tomato,Yuanyi Xuebao(Acta Horticulturae Sinica),44(1):120-130(刘根忠,王云飞,孟祥飞,张余洋,叶志彪,2017,EMS诱变的‘里格尔87-5’番茄M2群体突变体表型及其抗坏血酸研究,园艺学报,44(1):120-130)
Luo H.B.,Zhou J.,He C.A.,Li D.C.,Xiong Q.F.,Zhang X.K.,Zhang G.S.,and Li S.S.,2013,Primary study on mutagenic effect in radish by ethylmethylsulfone(EMS),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(5):608-611(骆海波,邹颉,贺从安,李德超,熊秋芳,张小康,张贵生,李世升,2013,甲基磺酸乙酯(EMS)对萝卜早期发育性状的诱变效应,基因组学与应用生物学,32(5):608-611)
Luo L.,Liang Y.,Cui X.,Yang J.,Sun Y.D.,Li C.,and Zheng S.X.,2012,The preliminary study on screening low-temperature-resistant mutants of tomato with chemical mutagenesis,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),28(22):138-143(罗雷,梁燕,崔霞,杨姣,孙亚东,李翠,郑戍翔,2012,化学诱变剂诱导番茄耐低温突变体的初步研究,中国农学通报,28(22):138-143)
Ma H.X.,Pang S.Q.,Yang B.J.,Yan L.J.,Wang B.,Liu P.,and Long Z.L.,2015,Effect of EMS mutagenesis on processing tomato seed germination,Zhongzi(Seed),34(5):28-33(马海新,庞胜群,杨邦杰,闫丽娟,汪斌,刘鹏,龙志林,2015,EMS诱变对加工番茄种子萌发的影响,种子,34(5):28-33)
Menda N.,Semel Y.,Peled D.,Eshed Y.,and Zamir D.,2004,In silico screening of a saturated mutation library of tomato,Plant J.,38(5):861-872
Minoia S.,Petrozza A.,D'Onofrio O.,Piron F.,Mosca G.,Sozio G.,Cellini F.,Bendahmane A.,and Carriero F.,2010,A new mutant genetic resource for tomato crop improvement by TILLING technology,BMC Research Notes,(3):69
Shang N.,2017,Construction and phenotypic analysis of EMSmutagenized tomato mutant library,Thesis M.S.,Northeast Agricultural University,Supervisor:Li J.F.,pp.10-11(尚娜,2017,EMS诱变番茄突变体库的构建及表型分析,硕士学位论文,东北农业大学,导师:李景富,pp.10-11)
Sternberg S.H.,Redding S.,Jinek M.,Greene E.C.,and Doudna J.A.,2014,DNA interrogation by the CRISPR RNA-guided endonuclease Cas9,Nature,507(7409):62-67
Yang J.H.,Cui X.,Chang P.P.,Li C.,Li Y.Z.,and Liang Y.,2014,Mutation phenotype identification and analysis of tomato inbred line TTD302A induced by EMS,Zhongguo Shucai(Chinese Vegetables),(4):21-28(杨建华,崔霞,常培培,李翠,李云洲,梁燕,2014,EMS诱变番茄自交系TTD-302A的突变表型鉴定和分析,中国蔬菜,(4):21-28)
Yang X.M.,2017,Construction of EMS mutant library and analysis of a yellowing leaf mutant in tomato,Thesis M.S.,Shenyang Agricultural University,Supervisor:Qi M.F.,pp.11-36(杨小苗,2017,番茄EMS突变体库的构建及叶色黄化突变体的分析,硕士学位论文,沈阳农业大学,导师:齐明芳,pp.11-36)
Zhang B.,2012,Preliminary probe into EMS mutagenesis cucumber(Cucumis sativus L.),Thesis M.S.,Shandong A-gricultural University,Supervisor:Ren Z.H.,pp.13-36(张兵,2012,EMS诱导黄瓜突变体的初步研究,硕士学位论文,山东农业大学,导师:任仲海,pp.13-36)
Zhou L.M.,Wang F.,and Wang J.,2009,Selection of salt-tolerantmutants from ethyl methane sulfonate mutagenized kiwi-fruit embryonic calli,Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),18(5):330-335,340(周立名,王飞,王佳,2009,EMS诱变处理定向筛选猕猴桃耐盐突变体研究,西北农业学报,18(5):330-335,340)
Han J.,2005,Studies on creation technique of germplasm resources in roses,Thesis M.S.,Shandong Agricultural University,Supervisor:Feng Z.,pp.4-7(韩进,2005,月季种质资源创新技术的研究,硕士学位论文,山东农业大学,导师:丰震,pp.4-7)
He H.Y.,Fan L.N.,Qi Y.W.,Lao F.Y.,Deng H.H.,and Hu F.,2016,Muta genesis application to Saccharum officinarum shoot tip culture with EMS,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),32(6):55-60(何慧怡,樊丽娜,齐永文,劳方业,邓海华,胡斐,2016,EMS诱变甘蔗茎尖分生组织的初步研究,中国农学通报,32(6):55-60)
Heigwer F.,Kerr G.,and Boutros M.,2014,E-CRISP:fastCRISPRtarget site identification,Nat.Methods,11(2):122-123
Liu G.Z.,Wang Y.F.,Meng X.F.,and Ye Z.B.,2017,Phenotype mutation and ascorbate characterization in M2population derived from EMS treatment in'Ligeer 87-5'tomato,Yuanyi Xuebao(Acta Horticulturae Sinica),44(1):120-130(刘根忠,王云飞,孟祥飞,张余洋,叶志彪,2017,EMS诱变的‘里格尔87-5’番茄M2群体突变体表型及其抗坏血酸研究,园艺学报,44(1):120-130)
Luo H.B.,Zhou J.,He C.A.,Li D.C.,Xiong Q.F.,Zhang X.K.,Zhang G.S.,and Li S.S.,2013,Primary study on mutagenic effect in radish by ethylmethylsulfone(EMS),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(5):608-611(骆海波,邹颉,贺从安,李德超,熊秋芳,张小康,张贵生,李世升,2013,甲基磺酸乙酯(EMS)对萝卜早期发育性状的诱变效应,基因组学与应用生物学,32(5):608-611)
Luo L.,Liang Y.,Cui X.,Yang J.,Sun Y.D.,Li C.,and Zheng S.X.,2012,The preliminary study on screening low-temperature-resistant mutants of tomato with chemical mutagenesis,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),28(22):138-143(罗雷,梁燕,崔霞,杨姣,孙亚东,李翠,郑戍翔,2012,化学诱变剂诱导番茄耐低温突变体的初步研究,中国农学通报,28(22):138-143)
Ma H.X.,Pang S.Q.,Yang B.J.,Yan L.J.,Wang B.,Liu P.,and Long Z.L.,2015,Effect of EMS mutagenesis on processing tomato seed germination,Zhongzi(Seed),34(5):28-33(马海新,庞胜群,杨邦杰,闫丽娟,汪斌,刘鹏,龙志林,2015,EMS诱变对加工番茄种子萌发的影响,种子,34(5):28-33)
Menda N.,Semel Y.,Peled D.,Eshed Y.,and Zamir D.,2004,In silico screening of a saturated mutation library of tomato,Plant J.,38(5):861-872
Minoia S.,Petrozza A.,D'Onofrio O.,Piron F.,Mosca G.,Sozio G.,Cellini F.,Bendahmane A.,and Carriero F.,2010,A new mutant genetic resource for tomato crop improvement by TILLING technology,BMC Research Notes,(3):69
Shang N.,2017,Construction and phenotypic analysis of EMSmutagenized tomato mutant library,Thesis M.S.,Northeast Agricultural University,Supervisor:Li J.F.,pp.10-11(尚娜,2017,EMS诱变番茄突变体库的构建及表型分析,硕士学位论文,东北农业大学,导师:李景富,pp.10-11)
Sternberg S.H.,Redding S.,Jinek M.,Greene E.C.,and Doudna J.A.,2014,DNA interrogation by the CRISPR RNA-guided endonuclease Cas9,Nature,507(7409):62-67
Yang J.H.,Cui X.,Chang P.P.,Li C.,Li Y.Z.,and Liang Y.,2014,Mutation phenotype identification and analysis of tomato inbred line TTD302A induced by EMS,Zhongguo Shucai(Chinese Vegetables),(4):21-28(杨建华,崔霞,常培培,李翠,李云洲,梁燕,2014,EMS诱变番茄自交系TTD-302A的突变表型鉴定和分析,中国蔬菜,(4):21-28)
Yang X.M.,2017,Construction of EMS mutant library and analysis of a yellowing leaf mutant in tomato,Thesis M.S.,Shenyang Agricultural University,Supervisor:Qi M.F.,pp.11-36(杨小苗,2017,番茄EMS突变体库的构建及叶色黄化突变体的分析,硕士学位论文,沈阳农业大学,导师:齐明芳,pp.11-36)
Zhang B.,2012,Preliminary probe into EMS mutagenesis cucumber(Cucumis sativus L.),Thesis M.S.,Shandong A-gricultural University,Supervisor:Ren Z.H.,pp.13-36(张兵,2012,EMS诱导黄瓜突变体的初步研究,硕士学位论文,山东农业大学,导师:任仲海,pp.13-36)
Zhou L.M.,Wang F.,and Wang J.,2009,Selection of salt-tolerantmutants from ethyl methane sulfonate mutagenized kiwi-fruit embryonic calli,Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),18(5):330-335,340(周立名,王飞,王佳,2009,EMS诱变处理定向筛选猕猴桃耐盐突变体研究,西北农业学报,18(5):330-335,340)