不同烤烟种质的烟碱含量与其农艺性状的相关性分析
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摘要
为探索适合烟碱品质改良的田间选育方法,本研究先通过对比三种烟叶处理方法对烟碱含量的影响,确定冷冻干燥新鲜烟叶方法作为样品处理方法。再选取116份烤烟品种作为供试群体,连续4年种植在南方试验点(四川西昌)。以简单相关分析、主成分分析方法对13个农艺性状与鲜烟叶烟碱含量的相关性进行评价。结果表明烤烟农艺性状的变异系数丰富,变异系数达12%～30%,烟碱含量的变异系数高达30%。经简单相关分析表明:株高与烟碱含量显著正相关,叶数、茎围、上二棚叶长宽比、上二棚叶长、下二棚叶宽共5个农艺性状与烟碱含量显著负相关。经主成分分析,结果显示:三个因子(烟叶长宽比,叶长,株高)是与烟碱含量相关的主要因子。因此,在西昌地区进行烟碱品质改良的田间选育时,宜将上二棚及腰叶的叶长宽比作为首要筛选标准,并综合考虑株高,叶数等性状。
The aim of this study is to explore new evaluation method for tobacco breeding. Firstly, we compared influence on nicotine content by three method of tobacco leaves treatment, then determined to use the method of freeze-drying fresh tobacco leaves as sample treatment method. Secondly, 13 agronomic traits and nicotine content of 116 flue-cured tobacco varieties were measured in 4 consecutive years in the southern test site(Xichang, Sichuan). Simple correlation analysis and principal component analysis were used to explore the relationship between nicotine content and these traits. The results showed that variations of main agronomic traits in these varieties were abundant with the coefficients of variation ranging from 12%～30%. The variation coefficient of nicotine content was up to 30%. Simple correlation analysis showed that nicotine content was significantly positively correlated with the plant height and negative correlated with leaves number, stem girth, top leaf length-width ration, top leaf length bottom leaf width. Principal component analysis showed the three factors(leaf length-width ration, leaf length and plant) were largely responsible for nicotine content. Therefore, when breeding for flue-cured tobacco varieties in Xichang test site, the length-width ration of top leaf and middle leaf should be taken as the primary screening criteria, then plant height and leaf number should be considered comprehensively.
The aim of this study is to explore new evaluation method for tobacco breeding. Firstly, we compared influence on nicotine content by three method of tobacco leaves treatment, then determined to use the method of freeze-drying fresh tobacco leaves as sample treatment method. Secondly, 13 agronomic traits and nicotine content of 116 flue-cured tobacco varieties were measured in 4 consecutive years in the southern test site(Xichang, Sichuan). Simple correlation analysis and principal component analysis were used to explore the relationship between nicotine content and these traits. The results showed that variations of main agronomic traits in these varieties were abundant with the coefficients of variation ranging from 12%～30%. The variation coefficient of nicotine content was up to 30%. Simple correlation analysis showed that nicotine content was significantly positively correlated with the plant height and negative correlated with leaves number, stem girth, top leaf length-width ration, top leaf length bottom leaf width. Principal component analysis showed the three factors(leaf length-width ration, leaf length and plant) were largely responsible for nicotine content. Therefore, when breeding for flue-cured tobacco varieties in Xichang test site, the length-width ration of top leaf and middle leaf should be taken as the primary screening criteria, then plant height and leaf number should be considered comprehensively.
引文
Cai B.,Jack A.M.,Lewis R.S.,Dewey R.E.,and Bush L.P.,2013,(R)-nicotine biosynthesis,metabolism and translocation in tobacco as determined by nicotine demethylase mutants,Phytochemistry,95(6):188-196
Cao J.L.,1996,Relationship between main appearance characters of leaf and chemical constituents and it's regulation techniques in oriental tobacco,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),3(1):78-80(曹景林,1996,香料烟叶片主要外观性状与化学成分关系及调控技术的研究,中国烟草学报,3(1):78-80)
Chen W.,and Jiang W.,2011,Correlation analysis on nicotine content and physical properties of flue-cured tobacco leaves,Nanfang Nongye Xuebao(Journal of Southern Agricultural),42(7):782-785(陈伟,蒋卫,2011,烤烟烟碱含量与烟叶物理性状的关联分析,南方农业学报,42(7):82-785)
Chen W.,Chen Y.,Huang L.,Li H.X.,Pan W.J.,and Li Z.Y.,2013,Effects of soil and climate on nicotine and potassium content of cured tobacco leaves,Turang(Soils),45(4):713-717(陈伟,陈懿,黄磊,李洪勋,潘文杰,李智勇,2013,土壤与气候对烤后烟叶烟碱和钾含量的影响,土壤,45(4):713-717)
Chen X.C.,Wang S.M.,Wang H.J.,and Wang S.,2015,Genetic analysis of rice grain mineral elements content and correlation analysis with main agronomic characters,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(3):460-466(陈秀晨,王士梅,王海娟,王申,2015,水稻子粒矿质元素含量遗传及主要农艺性状相关性分析,植物遗传资源学报,16(3):460-466)
Gu Y.M.,Cheng S.,Dou Y.Q.,Xie Y.,Liu G.,Liu X.M.,and Du Y.M.,2011,Relationship between leaf shape and inner quality of flue-cured tobacco in Xuanwei county of Yunnan province,Zhongguo Yancao Kexue(Chinese Tobacco Science),32(4):6-9(顾毓敏,程森,窦玉青,解燕,刘国,刘新民,杜咏梅,2011,云南宣威初烤烟叶叶形特征与内在品质的关系研究,中国烟草科学,32(4):6-9)
Huang P.,and Xu Q.G.,2016,The differences of agronomic and economic characters in different flue-cured tobacco cultivars,Zuowu Yanjiu(Crop Research),(2):126-130(黄鹏,徐庆国,2016,不同烤烟品种农艺性状与经济性状的差异研究,作物研究,(2):126-130)
Jiao J.,Gao R.F.,Wang D.W.,Han Y.Y.,Qiu X.M.,Jiang H.,and Guo F.Z.,2008,Analysis on factors affecting photosynthetic rate in different male sterility lines of wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(6):1622-1629(焦健,高庆荣,王大伟,郝媛媛,邱新民,姜辉,郭凤芝,2008,不同小麦雄性不育类型光合速率的影响因子分析,中国农业科学,41(6):1622-1629)
Jin Y.F.,Li J.Y.,Zhang J.B.,and Gong M.,2015,Biochemical and molecular mechanism of nicotine metabolism in tobacco plants,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(4):882-891(金云峰,李军营,张建波,龚明,2015,烟草烟碱代谢的生化和分子机制及其调控,基因组学与应用生物学,34(4):882-891)
Kong S.P.,Kong J.Q.,Wu X.,Yang Y.Y.,Huo Y.M.,and Xu K.,2015,Analysis of relationship between variations of main agronomic traits and yield in garlic,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),48(6):1240-1248(孔素萍,孙敬强,吴雄,杨妍妍,霍雨猛,徐坤,2015,大蒜主要农艺性状变异特征及其与产量相关构成分析,中国农业科学,48(6):1240-1248)
Li C.H.,Wang Y.Q.,Lu W.J.,and Wang L.H.,2015,The principal component and cluster analysis of agronomic traits of coix germplasm resources in Yunnan,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(2):277-287(李春花,王艳青,卢文洁,王莉花,2015,云南薏苡种质资源农艺性状的主成分和聚类分析,植物遗传资源学报,16(2):277-287)
Li D.L.,Xu Z.C.,Bi Q.W.,Cai B.,and He J.W.,2008,Relationship between nicotine content and physical property in fluecured tobacco leaves,Zhongguo Yancao Kexue(Acta Tabacaria Sinica),14(3):36-39(李东亮,许自成,毕庆文,蔡冰,何结望,2008,烤烟烟碱含量与物理性状的关系分析,中国烟草学报,14(3):36-39)
Li X.,Xu K.,Gong B.,Cao B.L.,and Wang Z.B.,2012,Multiple analysis of relationship of agronomic traits and yield formation in ginger,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),45(12):2431-2437(李秀,徐坤,巩彪,曹逼力,王忠宾,2012,生姜农艺性状与产量形成关系的多重分析,中国农业科学,45(12):2431-2437)
Shitan N.,Hayashida M.,and Yazaki K.,2015,Translocation and accumulation of nicotine via distinct spatio-temporal regulation of nicotine transporters in Nicotiana tabacum,Plant Signal.Behav.,10(7):e1035852
Sun X.,Wen L.Y.,Yin J.P.,Luo C.G.,Fan W.Q.,and Ren M.,2017,Genome-wide association study of nicotine content in flue-cured tobacco,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),23(1):102-110(孙鑫,文柳璎,尹鹏嘉,罗成刚,樊文强,任民,2017,烤烟烟碱含量的全基因组关联分析,中国烟草学报,23(1):102-110)
Wang C.,Hu D.,Yang H.,Ruan R.W.,Yuan X.H.,Yi Z.L.,Song Z.C.,and Zhao D.,2013,Multiple analysis of relationship between main agronomic traits and yield in tartary buckwheat,Zuowu Zazhi(Crops),(6):18-22(汪灿,胡丹,杨浩,阮仁武袁晓辉,易泽林,宋志成,赵丹,2013,苦荞主要农艺性状与产量关系的多重分析,作物杂志,(6):18-22)
Xiang C.P.,Xie J.,Nie Q.J.,and Wang L.P.,2001,Principal component analysis on characters of balsam pear,Huazhong Nongye Daxue Xuebao(Journal of Huazhong Agricultural University),20(4):378-381(向长萍,谢军,聂启军,汪李平,2001,23个苦瓜品种农艺性状的主成分分析,华中农业大学学报,20(4):378-81)
Yao Y.J.,Gao X.,Wu D.,Li X.Y.,Chen Q.J.,Dong J.,Zhao W.C.,Chen L.G.,Shi Y.G.,and Li X.J.,2014,Multivariate analysis and evaluation of agronomic and quality traits based on principal components in wheat,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),15(1):38-47(要燕杰,高翔,吴丹,李晓燕,陈其皎,董剑,赵万春,陈良国,石引刚,李学军,2014,小麦农艺性状与品质特性的多元分析与评价,植物遗传资源学报,15(1):38-47)
Yin P.J.,Ren M.,Sun X.,Zhou S.Q.,Yang A.G.,Luo C.G.,Chen A.G.,and Wen L.Y.,2018,Variation of main agronomic traits and correlation analysis of nicotine content in flue-cured tobacco,Zhongguo Yancao Kexue(Chinese Tobacco Science),39(1):10-16(尹鹏嘉,任民,孙鑫,周世奇,杨爱国,罗成刚,陈爱国,文柳璎,2018,烤烟主要农艺性状变异特征以及与烟碱含量的相关分析,中国烟草科学,39(1):10-16)
Yin Y.,Zhang Y.,Yu X.W.,Wang Y.,Yang A.G.,Chang A.X.,Luo C.G.,Li W.H.,and Feng L.,2012,Relationships among main agronomic attributes and yields and output value of flue-cured tobacco,Zhongguo Yancao Kexue(Chinese Tobacco Science),(6):18-22(殷英,张玉,余祥文,王勇,杨爱国,常爱霞,罗成刚,李卫华,冯丽,2012,烤烟主要农艺性状与产量产值的关系研究,中国烟草科学,(6):18-22)
Cao J.L.,1996,Relationship between main appearance characters of leaf and chemical constituents and it's regulation techniques in oriental tobacco,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),3(1):78-80(曹景林,1996,香料烟叶片主要外观性状与化学成分关系及调控技术的研究,中国烟草学报,3(1):78-80)
Chen W.,and Jiang W.,2011,Correlation analysis on nicotine content and physical properties of flue-cured tobacco leaves,Nanfang Nongye Xuebao(Journal of Southern Agricultural),42(7):782-785(陈伟,蒋卫,2011,烤烟烟碱含量与烟叶物理性状的关联分析,南方农业学报,42(7):82-785)
Chen W.,Chen Y.,Huang L.,Li H.X.,Pan W.J.,and Li Z.Y.,2013,Effects of soil and climate on nicotine and potassium content of cured tobacco leaves,Turang(Soils),45(4):713-717(陈伟,陈懿,黄磊,李洪勋,潘文杰,李智勇,2013,土壤与气候对烤后烟叶烟碱和钾含量的影响,土壤,45(4):713-717)
Chen X.C.,Wang S.M.,Wang H.J.,and Wang S.,2015,Genetic analysis of rice grain mineral elements content and correlation analysis with main agronomic characters,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(3):460-466(陈秀晨,王士梅,王海娟,王申,2015,水稻子粒矿质元素含量遗传及主要农艺性状相关性分析,植物遗传资源学报,16(3):460-466)
Gu Y.M.,Cheng S.,Dou Y.Q.,Xie Y.,Liu G.,Liu X.M.,and Du Y.M.,2011,Relationship between leaf shape and inner quality of flue-cured tobacco in Xuanwei county of Yunnan province,Zhongguo Yancao Kexue(Chinese Tobacco Science),32(4):6-9(顾毓敏,程森,窦玉青,解燕,刘国,刘新民,杜咏梅,2011,云南宣威初烤烟叶叶形特征与内在品质的关系研究,中国烟草科学,32(4):6-9)
Huang P.,and Xu Q.G.,2016,The differences of agronomic and economic characters in different flue-cured tobacco cultivars,Zuowu Yanjiu(Crop Research),(2):126-130(黄鹏,徐庆国,2016,不同烤烟品种农艺性状与经济性状的差异研究,作物研究,(2):126-130)
Jiao J.,Gao R.F.,Wang D.W.,Han Y.Y.,Qiu X.M.,Jiang H.,and Guo F.Z.,2008,Analysis on factors affecting photosynthetic rate in different male sterility lines of wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(6):1622-1629(焦健,高庆荣,王大伟,郝媛媛,邱新民,姜辉,郭凤芝,2008,不同小麦雄性不育类型光合速率的影响因子分析,中国农业科学,41(6):1622-1629)
Jin Y.F.,Li J.Y.,Zhang J.B.,and Gong M.,2015,Biochemical and molecular mechanism of nicotine metabolism in tobacco plants,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(4):882-891(金云峰,李军营,张建波,龚明,2015,烟草烟碱代谢的生化和分子机制及其调控,基因组学与应用生物学,34(4):882-891)
Kong S.P.,Kong J.Q.,Wu X.,Yang Y.Y.,Huo Y.M.,and Xu K.,2015,Analysis of relationship between variations of main agronomic traits and yield in garlic,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),48(6):1240-1248(孔素萍,孙敬强,吴雄,杨妍妍,霍雨猛,徐坤,2015,大蒜主要农艺性状变异特征及其与产量相关构成分析,中国农业科学,48(6):1240-1248)
Li C.H.,Wang Y.Q.,Lu W.J.,and Wang L.H.,2015,The principal component and cluster analysis of agronomic traits of coix germplasm resources in Yunnan,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(2):277-287(李春花,王艳青,卢文洁,王莉花,2015,云南薏苡种质资源农艺性状的主成分和聚类分析,植物遗传资源学报,16(2):277-287)
Li D.L.,Xu Z.C.,Bi Q.W.,Cai B.,and He J.W.,2008,Relationship between nicotine content and physical property in fluecured tobacco leaves,Zhongguo Yancao Kexue(Acta Tabacaria Sinica),14(3):36-39(李东亮,许自成,毕庆文,蔡冰,何结望,2008,烤烟烟碱含量与物理性状的关系分析,中国烟草学报,14(3):36-39)
Li X.,Xu K.,Gong B.,Cao B.L.,and Wang Z.B.,2012,Multiple analysis of relationship of agronomic traits and yield formation in ginger,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),45(12):2431-2437(李秀,徐坤,巩彪,曹逼力,王忠宾,2012,生姜农艺性状与产量形成关系的多重分析,中国农业科学,45(12):2431-2437)
Shitan N.,Hayashida M.,and Yazaki K.,2015,Translocation and accumulation of nicotine via distinct spatio-temporal regulation of nicotine transporters in Nicotiana tabacum,Plant Signal.Behav.,10(7):e1035852
Sun X.,Wen L.Y.,Yin J.P.,Luo C.G.,Fan W.Q.,and Ren M.,2017,Genome-wide association study of nicotine content in flue-cured tobacco,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),23(1):102-110(孙鑫,文柳璎,尹鹏嘉,罗成刚,樊文强,任民,2017,烤烟烟碱含量的全基因组关联分析,中国烟草学报,23(1):102-110)
Wang C.,Hu D.,Yang H.,Ruan R.W.,Yuan X.H.,Yi Z.L.,Song Z.C.,and Zhao D.,2013,Multiple analysis of relationship between main agronomic traits and yield in tartary buckwheat,Zuowu Zazhi(Crops),(6):18-22(汪灿,胡丹,杨浩,阮仁武袁晓辉,易泽林,宋志成,赵丹,2013,苦荞主要农艺性状与产量关系的多重分析,作物杂志,(6):18-22)
Xiang C.P.,Xie J.,Nie Q.J.,and Wang L.P.,2001,Principal component analysis on characters of balsam pear,Huazhong Nongye Daxue Xuebao(Journal of Huazhong Agricultural University),20(4):378-381(向长萍,谢军,聂启军,汪李平,2001,23个苦瓜品种农艺性状的主成分分析,华中农业大学学报,20(4):378-81)
Yao Y.J.,Gao X.,Wu D.,Li X.Y.,Chen Q.J.,Dong J.,Zhao W.C.,Chen L.G.,Shi Y.G.,and Li X.J.,2014,Multivariate analysis and evaluation of agronomic and quality traits based on principal components in wheat,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),15(1):38-47(要燕杰,高翔,吴丹,李晓燕,陈其皎,董剑,赵万春,陈良国,石引刚,李学军,2014,小麦农艺性状与品质特性的多元分析与评价,植物遗传资源学报,15(1):38-47)
Yin P.J.,Ren M.,Sun X.,Zhou S.Q.,Yang A.G.,Luo C.G.,Chen A.G.,and Wen L.Y.,2018,Variation of main agronomic traits and correlation analysis of nicotine content in flue-cured tobacco,Zhongguo Yancao Kexue(Chinese Tobacco Science),39(1):10-16(尹鹏嘉,任民,孙鑫,周世奇,杨爱国,罗成刚,陈爱国,文柳璎,2018,烤烟主要农艺性状变异特征以及与烟碱含量的相关分析,中国烟草科学,39(1):10-16)
Yin Y.,Zhang Y.,Yu X.W.,Wang Y.,Yang A.G.,Chang A.X.,Luo C.G.,Li W.H.,and Feng L.,2012,Relationships among main agronomic attributes and yields and output value of flue-cured tobacco,Zhongguo Yancao Kexue(Chinese Tobacco Science),(6):18-22(殷英,张玉,余祥文,王勇,杨爱国,常爱霞,罗成刚,李卫华,冯丽,2012,烤烟主要农艺性状与产量产值的关系研究,中国烟草科学,(6):18-22)
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