玉米o2,o16和wx基因不同聚合类型近等基因系的选育
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摘要
为研究玉米高赖氨酸奥帕克-2(opaque-2,o2),奥帕克-16(opaque-16,o16)和糯性(waxy1,wx1)突变基因聚合提高营养品质的分子机理,以玉米o2,o16和wx三隐性基因聚合系QCL 8002-11(o2o2o16o16wxwx)为供体亲本,以普通玉米自交系CML 539为受体亲本,构建玉米o2、o16和wx基因不同聚合类型的近等基因系,经过多代回交和自交,利用分子标记辅助选择技术,选育出一批含有o2o16wx三隐性基因,o2o16、o2wx和o16wx双隐性基因,o2、o16和wx单隐性基因的近等基因系材料,为玉米品质的快速改良和阐明优异基因聚合提高玉米营养品质的机理提供材料基础。
To dissect molecular mechanism improving the nutritional quality in maize through pyramiding high-lysine mutant genes(opaque2 and opaque16)and waxy mutant gene(waxy1),the o2,o16 and wx genes were introgressed into normal line using three recessive gene mutant(QCL 8002-11,o2 o2 o16 o16 wxwx)as donor and normal line(CML 539)as a receptor.Using molecular marker-assisted selection technology,near-isogenic lines(NILs)of three recessive gene mutant(o2 o16 wx),double recessive gene mutants(o2 o16,o2 wxand o16 wx)and single recessive gene mutants(o2,o16 and wx)were bred by multiple backcross and self-pollination.These NILs will provide materials for fast quality improvement and elucidating the molecular mechanisms underlying the nutritional quality in maize.
To dissect molecular mechanism improving the nutritional quality in maize through pyramiding high-lysine mutant genes(opaque2 and opaque16)and waxy mutant gene(waxy1),the o2,o16 and wx genes were introgressed into normal line using three recessive gene mutant(QCL 8002-11,o2 o2 o16 o16 wxwx)as donor and normal line(CML 539)as a receptor.Using molecular marker-assisted selection technology,near-isogenic lines(NILs)of three recessive gene mutant(o2 o16 wx),double recessive gene mutants(o2 o16,o2 wxand o16 wx)and single recessive gene mutants(o2,o16 and wx)were bred by multiple backcross and self-pollination.These NILs will provide materials for fast quality improvement and elucidating the molecular mechanisms underlying the nutritional quality in maize.
引文
[1]Mertz E T,Bates L S,Nelson O E.Mutant gene that changes protein composition and increases lysine content of maize endosperm[J].Science,1964,145(3629):279-280.
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[4]Coleman C E,Clore A M,Ranch J P,et al.Expression of a mutant alpha-zein creates the floury 2phenotype in transgenic maize[J].Proc Natl Acad Sci,1997,94:7094-7097.
[5]Pirona R,Hartings H,Lauria M,et al.Genetic control of endosperm development and of storage products accumulation in maize seeds[J].Maydica,2005,50:515-530.
[6]Nelson O E.The location of fl 3on chromosome 8[J].Maize Genet Coop Newslett,1976,50:114.
[7]Teas H J,Teas A N.Heritable characters in maize:Description and linkage of brittle endosperm-2[J].J Hered,1953,44:156-158.
[8]Robertson D S.A new opaque gene located on chromosome 7[J].Maize Genet Coop News Letter,1967,41:94-95.
[9]Myers A M,James M G,Lin Q,et al.Maize opaque5encodes monogalactosyldiacylglycerol synthase and specifically affects galactolipids necessary for amyloplast and chloroplast function[J].Plant Cell,2011,23:2331-2347.
[10]Ashman R B.Gene linkages in translocation T 9-10aheterozygotes[J].Maize Genet Coop News Letter,1968,42:155-156.
[11]Ma Y,Nelson O E.Amino acid composition and storage proteins in two new high-lysine mutants in maize[J].Cereal Chem,1975,52:412-419.
[12]Wang G,Zhang J S,Wang G F,et al.Proline responding 1plays a critical role in regulating general protein synthesis and the cell cycle in maize[J].Plant Cell,2014,26:2582-2600.
[13]Salamini F,Di Fonzo N,Fornasari E et al.Mucronate,Mc.a dominant gene of maize which interacts with opaque-2to suppress zein synthesis[J].Theor Appl Genet,1983,65:123-128.
[14]Yang W P,Zheng Y L,Zheng W T,et al.Molecular genetic mapping of a high-lysine mutant gene(opaque-16)and the double recessive effect with opaque-2in maize[J].Mol Breeding,2005,15:257-269.
[15]Sarika K,Hossain F,Muthusamy V,et al.Exploration of novel opaque16mutation as a source for high-lysine andtryptophan in maize endosperm[J].Indian J Genet,2017,77:59-64.
[16]Sarika K,Hossain F,Muthusamy V,et al.Opaque 16,a high lysine and tryptophan mutant,does not influence the key physico-biochemical characteristics in maize kernel[J].PloS ONE,2018,13:e0190945.
[17]Sarika K,Hossain F,Muthusamy V,et al.Marker-assisted pyramiding of opaque 2and novel opaque16genes for further enrichment of lysine and tryptophan in sub-tropical maize[J].Plant Sci,2018,272:142-152.
[18]Zhang W L,Yang W P,Chen Z W,et al.Molecular marker-assisted selection for o 2introgression lines with o16gene in corn[J].Acta Agronomica Sinica,2010,36:1302-1309.
[19]Zhang X X,Zhu H,Zhang D M,et al.Construction of waxy maize opaque 2near-isogenic lines[J].Acta Agronomica Sinica,2017,43:1760-1766.
[20]Yang L Q,Wang W,Yang W P,et al.Marker-assisted selection for pyramiding the waxy and opaque-16genes in maize using cross and backcross schemes[J].Mol Breeding,2013,31:767-775.
[21]Zhang W,Yang W,Wang M,et al.Increasing lysine content of waxy maize through introgression of opaque-2and opaque-16genes using molecular assisted and biochemical development[J].PloS ONE,2012,8:e 56227.
[22]王伟,杨文鹏,王明春.优质蛋白糯玉米自交系的选育及品质分析[J].种子,2017,36(4):105-108.
[2]Mains E B.Heritable characters in maize:Linkage of a factor for shrunken endosperm with the a1factor for aleurone color[J].J Hered,1949,40:21-24.
[3]Nelson O E,Mertz E T,Bates L S.Second mutant gene affecting the amino acid pattern of maize endosperm proteins[J].Science,1965,150:1469-1470.
[4]Coleman C E,Clore A M,Ranch J P,et al.Expression of a mutant alpha-zein creates the floury 2phenotype in transgenic maize[J].Proc Natl Acad Sci,1997,94:7094-7097.
[5]Pirona R,Hartings H,Lauria M,et al.Genetic control of endosperm development and of storage products accumulation in maize seeds[J].Maydica,2005,50:515-530.
[6]Nelson O E.The location of fl 3on chromosome 8[J].Maize Genet Coop Newslett,1976,50:114.
[7]Teas H J,Teas A N.Heritable characters in maize:Description and linkage of brittle endosperm-2[J].J Hered,1953,44:156-158.
[8]Robertson D S.A new opaque gene located on chromosome 7[J].Maize Genet Coop News Letter,1967,41:94-95.
[9]Myers A M,James M G,Lin Q,et al.Maize opaque5encodes monogalactosyldiacylglycerol synthase and specifically affects galactolipids necessary for amyloplast and chloroplast function[J].Plant Cell,2011,23:2331-2347.
[10]Ashman R B.Gene linkages in translocation T 9-10aheterozygotes[J].Maize Genet Coop News Letter,1968,42:155-156.
[11]Ma Y,Nelson O E.Amino acid composition and storage proteins in two new high-lysine mutants in maize[J].Cereal Chem,1975,52:412-419.
[12]Wang G,Zhang J S,Wang G F,et al.Proline responding 1plays a critical role in regulating general protein synthesis and the cell cycle in maize[J].Plant Cell,2014,26:2582-2600.
[13]Salamini F,Di Fonzo N,Fornasari E et al.Mucronate,Mc.a dominant gene of maize which interacts with opaque-2to suppress zein synthesis[J].Theor Appl Genet,1983,65:123-128.
[14]Yang W P,Zheng Y L,Zheng W T,et al.Molecular genetic mapping of a high-lysine mutant gene(opaque-16)and the double recessive effect with opaque-2in maize[J].Mol Breeding,2005,15:257-269.
[15]Sarika K,Hossain F,Muthusamy V,et al.Exploration of novel opaque16mutation as a source for high-lysine andtryptophan in maize endosperm[J].Indian J Genet,2017,77:59-64.
[16]Sarika K,Hossain F,Muthusamy V,et al.Opaque 16,a high lysine and tryptophan mutant,does not influence the key physico-biochemical characteristics in maize kernel[J].PloS ONE,2018,13:e0190945.
[17]Sarika K,Hossain F,Muthusamy V,et al.Marker-assisted pyramiding of opaque 2and novel opaque16genes for further enrichment of lysine and tryptophan in sub-tropical maize[J].Plant Sci,2018,272:142-152.
[18]Zhang W L,Yang W P,Chen Z W,et al.Molecular marker-assisted selection for o 2introgression lines with o16gene in corn[J].Acta Agronomica Sinica,2010,36:1302-1309.
[19]Zhang X X,Zhu H,Zhang D M,et al.Construction of waxy maize opaque 2near-isogenic lines[J].Acta Agronomica Sinica,2017,43:1760-1766.
[20]Yang L Q,Wang W,Yang W P,et al.Marker-assisted selection for pyramiding the waxy and opaque-16genes in maize using cross and backcross schemes[J].Mol Breeding,2013,31:767-775.
[21]Zhang W,Yang W,Wang M,et al.Increasing lysine content of waxy maize through introgression of opaque-2and opaque-16genes using molecular assisted and biochemical development[J].PloS ONE,2012,8:e 56227.
[22]王伟,杨文鹏,王明春.优质蛋白糯玉米自交系的选育及品质分析[J].种子,2017,36(4):105-108.