光氧环境对紫色硫细菌YL28去除无机三态氮的影响
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摘要
【背景】光和氧是制约光合细菌生长代谢进而影响其除氮效果的重要因素。不产氧光合细菌紫色硫细菌——海洋着色菌(Marichromatium gracile) YL28能以亚硝氮为唯一氮源进行光合生长,对高浓度无机三态氮具有良好去除能力。【目的】阐明YL28菌株除氮效率与光氧环境的交互联系,获得其生物除氮的最适光氧条件。【方法】以高浓度无机三态氮共存海水水体为研究体系,在有光/无光条件下考查装样量(表征体系溶氧状态)对YL28菌株生物除氮活性的影响,并通过响应面分析法对装样量、光照强度和光周期3个主要因素进行优化。【结果】光照且氧浓度较低时(80%装样量),YL28具有最佳生长和无机三态氮去除能力;装样量在10%-100%时,菌体生物量(OD_(660))在0.938-2.719之间,当氨氮、亚硝氮和硝氮分别为7.16、5.67和4.83mmol/L时,其去除率分别在71.44%-89.09%、99.22%-99.83%和91.60%-97.33%。黑暗条件下,装样量在20%-100%时,氨氮、亚硝氮和硝氮去除率分别在48.07%-64.27%、73.51%-86.42%和42.57%-46.34%,但菌体生物量(OD_(660)为0.615-0.903)明显降低。通过响应面优化,当装样量、光照强度和光周期分别为80.0%(溶氧量约为0.32 mg/L)、2 800 lx和24L:0D时,细胞生长和氨氮去除活性达到最佳状态,分别比优化前提高了21.28%和14.11%。在实际应用中,选取72%-89%装样量(溶氧量约为0.26-0.63mg/L)、2240-3460lx光照强度和21L:3D-24L:0D光周期,细胞活性可达95%以上。【结论】80%装样量有助于促进菌体光照生长和除氮;在黑暗有氧和无氧环境下,YL28菌株也具有较好除氮活性,这为不产氧光合细菌在生物反应器中高效去除无机三态氮的应用提供了有价值的参考数据。
[Background] Light and oxygen are important limited factors for cell growth and the inorganic nitrogen removal of anoxygenic phototrophic bacteria(APB). Marichromatium gracile YL28, a member of purple sulfur bacteria in APB, can grow with nitrite as the sole nitrogen source and efficiently remove inorganic nitrogen. [Objective] This work aims to explore the relationship between light and/or oxygen and the removal efficiency of inorganic nitrogen by YL28, and acquire the optimal condition of light and oxygen for biological nitrogen removal processes. [Methods] A simulated seawater system with high concentration of co-exist inorganic nitrogen was used to investigate the effect of sample loading quantity(SLQ, it represents dissolved oxygen level) on the inorganic nitrogen removal efficiency by YL28 under the light or dark conditions. The three major factors of SLQ, light intensity and photoperiod were optimized by the response surface analysis. [Results] Under the light condition, the highest removal efficiency of inorganic nitrogen and cell growth were achieved at 80% of SLQ. When SLQ was in the range of 10% to 100%, the range of biomass value(OD_(660)) was from 0.938 to 2.719 and the removal rate of ammonia, nitrite and nitrate reached 71.44%-89.09%, 99.22%-99.83% and 91.60%-97.33% when exposed to 7.16, 5.67, 4.83 mmol/L of ammonia, nitrite, nitrate, respectively. Under the dark condition, when SLQ was in the range of 20% to100%, the removal rate of ammonia, nitrite and nitrate reached 48.07%-64.27%, 73.51%-86.42% and42.57%-46.34%, respectively, however, the biomass(OD_(660), 0.615-0.903) decreased significantly. The response surface analysis showed that the cell growth and ammonia removal efficiency were increased by21.28% and 14.11% under the 80.0% of SLQ(dissolved oxygen(DO) is approximately 0.32 mg/L),2 800 lx of light intensity and 24 L:0 D of photoperiod condition. In practical application, more than 95% of cell activity was achieved under 72% to 89% of SLQ(DO is in the range of 0.26 to 0.63 mg/L), 2 240-3 460 lx of light intensity and 21 L:3 D-24 L:0 D of photoperiod conditions. [Conclusion] The 80% of SLQ is of benefit to YL28's phototrophic growth and the inorganic nitrogen removal efficiency; YL28 remains the good nitrogen removal activity under anaerobically and/or aerobically in the dark. This study provides useful information for the removal of inorganic nitride by APB in bioreactor.
[Background] Light and oxygen are important limited factors for cell growth and the inorganic nitrogen removal of anoxygenic phototrophic bacteria(APB). Marichromatium gracile YL28, a member of purple sulfur bacteria in APB, can grow with nitrite as the sole nitrogen source and efficiently remove inorganic nitrogen. [Objective] This work aims to explore the relationship between light and/or oxygen and the removal efficiency of inorganic nitrogen by YL28, and acquire the optimal condition of light and oxygen for biological nitrogen removal processes. [Methods] A simulated seawater system with high concentration of co-exist inorganic nitrogen was used to investigate the effect of sample loading quantity(SLQ, it represents dissolved oxygen level) on the inorganic nitrogen removal efficiency by YL28 under the light or dark conditions. The three major factors of SLQ, light intensity and photoperiod were optimized by the response surface analysis. [Results] Under the light condition, the highest removal efficiency of inorganic nitrogen and cell growth were achieved at 80% of SLQ. When SLQ was in the range of 10% to 100%, the range of biomass value(OD_(660)) was from 0.938 to 2.719 and the removal rate of ammonia, nitrite and nitrate reached 71.44%-89.09%, 99.22%-99.83% and 91.60%-97.33% when exposed to 7.16, 5.67, 4.83 mmol/L of ammonia, nitrite, nitrate, respectively. Under the dark condition, when SLQ was in the range of 20% to100%, the removal rate of ammonia, nitrite and nitrate reached 48.07%-64.27%, 73.51%-86.42% and42.57%-46.34%, respectively, however, the biomass(OD_(660), 0.615-0.903) decreased significantly. The response surface analysis showed that the cell growth and ammonia removal efficiency were increased by21.28% and 14.11% under the 80.0% of SLQ(dissolved oxygen(DO) is approximately 0.32 mg/L),2 800 lx of light intensity and 24 L:0 D of photoperiod condition. In practical application, more than 95% of cell activity was achieved under 72% to 89% of SLQ(DO is in the range of 0.26 to 0.63 mg/L), 2 240-3 460 lx of light intensity and 21 L:3 D-24 L:0 D of photoperiod conditions. [Conclusion] The 80% of SLQ is of benefit to YL28's phototrophic growth and the inorganic nitrogen removal efficiency; YL28 remains the good nitrogen removal activity under anaerobically and/or aerobically in the dark. This study provides useful information for the removal of inorganic nitride by APB in bioreactor.
引文
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[4]Ni ZF,Wu XG,Li LF,et al.Pollution control and in situ bioremediation for lake aquaculture using an ecological dam[J].Journal of Cleaner Production,2018,172:2256-2265
[5]Luo S,Wu BL,Xiong XQ,et al.Short-term toxicity of ammonia,nitrite,and nitrate to early life stages of the rare minnow(Gobiocypris rarus)[J].Environmental Toxicology and Chemistry,2016,35(6):1422-1427
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[7]Thamdrup B.New pathways and processes in the global nitrogen cycle[J].Annual Review of Ecology,Evology,and Systematics,2012,43(1):407-428
[8]Pan YY,Zhou BH,Ma FS,et al.Secondary effluent of dying wastewater treatment plant with sulfur autotrophic denitrification[J].Chinese Journal of Environmental Engineering,2017,11(7):4073-4078(in Chinese)潘永月,周北海,马方曙,等.印染废水处理厂二级出水的硫自养反硝化脱氮工艺[J].环境工程学报,2017,11(7):4073-4078
[9]Bi CX,Yu DS,Du SM,et al.Nitrite accumulation characteristics of partial denitrification in different sludge sources using sodium acetate as carbon source[J].Environmental Science,2019,DOI:10.13227/j.hjkx.201806166(in Chinese)毕春雪,于德爽,杜世明,等.乙酸钠作为碳源不同污泥源短程反硝化过程亚硝酸盐积累特性[J].环境科学,2019,DOI:10.13227/j.hjkx.201806166
[10]Li T,Cao JW,Xie FL,et al.ABR Decarbonization-nitrosation coupled with anammox treat municipal wastewater[J].Environmental Science,2019.DOI:10.13227/j.hjkx.201808126(in Chinese)李田,曹家炜,谢凤莲,等.ABR除碳-亚硝化耦合厌氧氨氧化处理城市污水[J].环境科学,2019.DOI:10.13227/j.hjkx.201808126
[11]Zhang XB,Zhu BT,Chan ZH,et al.Effect of organic carbons on the removal of inorganic nitrogen coexisting in marine aquaculture by a marine purple sulfur bacterium,Marichramatium gracile YL28[J].Microbiology China,2017,44(5):1017-1027(in Chinese)张晓波,朱笔通,产竹华,等.有机碳对海洋着色菌YL28去除无机三态氮的影响[J].微生物学通报,2017,44(5):1017-1027
[12]Gardner WS,Mc Carthy MJ.Nitrogen dynamics at the sediment-water interface in shallow,sub-tropical Florida Bay:why denitrification efficiency may decrease with increased eutrophication[J].Biogeochemistry,2009,95(2/3):185-198
[13]Strauss EA,Richardson WB,Cavanaugh JC,et al.Variability and regulation of denitrification in an Upper Mississippi River backwater[J].Journal of the North American Benthological Society,2006,25(3):596-606
[14]Zheng XF,Tang JY,Zhang CF,et al.Bacterial composition,abundance and diversity in fish polyculture and mussel-fish integrated cultured ponds in China[J].Aquaculture Research,2017,48(7):3950-3961
[15]Zheng XF,Tang JY,Ren G,et al.The effect of four microbial products on production performance and water quality in integrated culture of freshwater pearl mussel and fishes[J].Aquaculture Research,2017,48(9):4897-4909
[16]Yang W,Zheng C,Zheng ZM,et al.Nutrient enrichment during shrimp cultivation alters bacterioplankton assemblies and destroys community stability[J].Ecotoxicology and Environmental Safety,2018,156:366-374
[17]Jiang P,Zhao CG,Yang SP.Influences of low-molecular-weight organic carbon and nitrogen sources on growth and inorganic nitrogen removal by Marichromatium gracile strain YL28[J].Oceanologia et Limnologia Sinica,2014,45(6):1218-1224(in Chinese)蒋鹏,赵春贵,杨素萍.小分子有机碳、氮源对海洋着色菌(Marichromatium gracile)生长和去除高浓度无机三态氮的影响[J].海洋与湖沼,2014,45(6):1218-1224
[18]Zhao JY,Fu YN,Zhao CG,et al.Identification and characterization of a purple sulfur bacterium from mangrove with rhodopin as predominant carotenoid[J].Acta Microbiologica Sinica,2011,51(10):1318-1325(in Chinese)赵江艳,傅英楠,赵春贵,等.一株高含玫红品的红树林海洋紫色硫细菌分离鉴定及特性[J].微生物学报,2011,51(10):1318-1325
[19]Jiang P,Zhao CG,Jia YQ,et al.Inorganic nitrogen removal by a marine purple sulfur bacterium capable of growth on nitrite as sole nitrogen source[J].Microbiology China,2014,41(5):824-831(in Chinese)蒋鹏,赵春贵,贾雅琼,等.以亚硝氮为唯一氮源生长的海洋紫色硫细菌去除无机三态氮[J].微生物学通报,2014,41(5):824-831
[20]Jiang P,Zhao CG,Jia YQ,et al.Effects of nitrite on ammonia-nitrogen removal and nitrite-nitrogen as well as photopigment biosynthesis of Marichromatium gracile YL28[J].Microbiology China,2015,42(7):1216-1223(in Chinese)蒋鹏,赵春贵,贾雅琼,等.亚硝氮对海洋着色菌亚硝氮和氨氮去除以及光合色素合成的影响[J].微生物学通报,2015,42(7):1216-1223
[21]Jiang P,Hong X,Zhao CG,et al.Reciprocal transformation of inorganic nitrogen by resting cells of Marichromatium gracile YL28[J].Journal of Huaqiao University(Natural Science),2015,36(2):185-189(in Chinese)蒋鹏,洪璇,赵春贵,等.海洋着色菌Marichromatium gracile YL28静息细胞对无机三态氮的相互转化作用[J].华侨大学学报:自然科学版,2015,36(2):185-189
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