牛疱疹病毒糖蛋白D的结构特点及其在疫苗研发中的应用
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摘要
来自牛疱疹病毒1和5型(BoHV-1和BoHV-5)的病毒囊膜糖蛋白D(gD)是病毒的主要组成成分,并且在疱疹病毒的致病机理中起到关键作用。gD对于病毒侵入细胞是必不可少的,是病毒感染细胞期间中和抗体的主要靶标。鉴于其在诱导保护性免疫方面的作用,已使用该糖蛋白作为主要抗原开发出亚单位疫苗、DNA疫苗和载体疫苗候选物,证明gD具有在宿主中诱导较强病毒中和抗体和细胞介导的免疫应答以及对宿主进行免疫保护的能力。论文着重介绍了BoHV-1、BoHV-5和一些人类疱疹病毒gD的结构和功能特征,阐述了gD与宿主免疫系统的反应及gD在新型疫苗设计中的应用,为全面了解gD结构特点及其在疫苗研发中的作用提供借鉴。
The viral envelope glycoprotein D from bovine herpesviruses 1 and 5(BoHV-1 and BoHV-5)is a major component of the virion and plays a critical role in the pathogenesis of herpesviruses.Glycoprotein D is essential for virus penetration into permissive cells and thus is a major target for virus neutralizing antibodies during infection.In view of its role in the induction of protective immunity,gD has been tested in new vaccine development strategies against both viruses.Subunit,DNA and vectored vaccine candidates have been developed using this glycoprotein as the primary antigen,demonstrating that gD has the capacity to induce robust virus neutralizing antibodies and strong cell-mediated immune responses,as well as protection from clinical symptoms,in target species.This review highlighted the structural and functional characteristics of BoHV-1,BoHV-5 and some human herpesvirus gD and described the response of gD to the host immune system and the application of gD in new vaccine design.This provided a reference for a comprehensive understanding of the characteristics of gD structure and its role in vaccine development.
The viral envelope glycoprotein D from bovine herpesviruses 1 and 5(BoHV-1 and BoHV-5)is a major component of the virion and plays a critical role in the pathogenesis of herpesviruses.Glycoprotein D is essential for virus penetration into permissive cells and thus is a major target for virus neutralizing antibodies during infection.In view of its role in the induction of protective immunity,gD has been tested in new vaccine development strategies against both viruses.Subunit,DNA and vectored vaccine candidates have been developed using this glycoprotein as the primary antigen,demonstrating that gD has the capacity to induce robust virus neutralizing antibodies and strong cell-mediated immune responses,as well as protection from clinical symptoms,in target species.This review highlighted the structural and functional characteristics of BoHV-1,BoHV-5 and some human herpesvirus gD and described the response of gD to the host immune system and the application of gD in new vaccine design.This provided a reference for a comprehensive understanding of the characteristics of gD structure and its role in vaccine development.
引文
[1] 高俊,刘荻萩,马月,等.疱疹病毒潜伏相关转录体的生物学特性[J].中国预防兽医学报,2017(10):855-860.
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[13] Gabev E,Tobler K,Abril C,et al.Glycoprotein D of bovine herpesvirus 5(BoHV-5) confers an extended host range to BoHV-1 but does not contribute to invasion of the brain[J].J Virol,2010,84:5583-5593.
[14] Newcomer B W,Cofield L G,Walz P H,et al.Prevention of abortion in cattle following vaccination against bovine herpesvirus 1:A meta-analysis[J].Prev Vet Med,2017,138:1-8.
[15] Siedler B S,Roloff B C,de Sá G L,et al.Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle[J].Protein Expr Purif,2017,130:21-27.
[16] Silvestro C,Bratanich A.The latency related gene of bovine herpesvirus types 1 and 5 and its modulation of cellular processes[J].Arch Virol,2016,161(12):3299-3308.
[17] Chase C C L,Fulton R W,O'Toole D,et al.Bovine herpesvirus 1 modified live virus vaccines for cattle reproduction:Balancing protection with undesired effects[J].Vet Microbiol,2017,206:69-77.
[18] 冷雪,郭利,张淑琴,等.牛传染性鼻气管炎活疫苗安全性和免疫保护效果研究[J].中国畜牧兽医,2011,38(10):181-184.
[19] Zhu L,Thompson J,Ma F,et al.Effects of the synthetic corticosteroid dexamethasone on bovine herpesvirus 1 productive infection[J].Virology,2017,505:71-79.
[20] 霍志云,胡嘉欣,童钦,等.Ⅰ型牛疱疹病毒潜伏感染机制研究进展[J].中国人兽共患病学报,2012,28(1):70-73.
[21] 刘瑞宁.牛传染性鼻气管炎疫苗研究进展[J].动物医学进展,2016,37(2):85-90.
[22] Shahin F,Raza S,Yang K,et al.Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading[J].Oncotarget,2017,8(55):94462-94480.
[23] Van Drunen Littel-van den Hurk,Lawman Z,Wilson D,et al.Electroporation enhances immune responses and protection induced by a bovine viral diarrhea virus DNA vaccine in newborn calves with maternal antibodies [J].Vaccine,2010,28:6445-6454.
[24] Skoberne M,Cardin R,Lee A,et al.An adjuvanted herpes simplex virus 2 subunit vaccine elicits a T cell response in mice and is an effective therapeutic vaccine in Guinea pigs[J].J Virol,2013,87:3930-3942.
[25] Ayalew L E,Kumar P,Gaba A,et al.Bovine adenovirus-3 as a vaccine delivery vehicle[J].Vaccine,2015,33(4):493-499.
[26] Tuncer-G?ktuna P,Alpay G,?ner E B,et al.The role of herpesviruses (BoHV-1 and BoHV-4) and pestiviruses (BVDV and BDV) in ruminant abortion cases in western Turkey[J].Trop Anim Health Prod,2016,48(5):1021-1027.
[27] Rosamilia A,Jacca S,Tebaldi G,et al.BoHV-4-based vector delivering Ebola virus surface glycoprotein[J].J Transl Med,2016,14(1):325.
[28] Lima L R,Silva A P,Schmidt-Chanasit J,et al.Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2):use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction[J].Mem Inst Oswaldo Cruz,2017,112(3):220-223.
[2] Kramer L M,Mayes M S,Fritz-Waters E,et al.Evaluation of responses to vaccination of Angus cattle for four viruses that contribute to bovine respiratory disease complex[J].J Anim Sci,2017,95(11):4820-4834.
[3] 李海涛,苗利光,朱言柱,等.牛传染性鼻气管炎病毒JZ06-8株犊牛感染模型的建立[J].动物医学进展,2015,36(6):115-118.
[4] Marin M S,Quintana S,Leunda M R,et al.A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis[J].J Virol Methods,2016,227:14-22.
[5] Del Medico Zajac M P,Zanetti F A,Esusy M S,et al.Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D[J].Viral Immunol,2017,30(1):70-76.
[6] Earley B,Tiernan K,Duffy C,et al.Effect of suckler cow vaccination against glycoprotein E (gE)-negative bovine herpesvirus type 1(BoHV-1) on passive immunity and physiological response to subsequent bovine respiratory disease vaccination of their progeny[J].Res Vet Sci,2018,118:43-51.
[7] Campos F S,Dezen D,Antunes D A,et al.Efficacy of an inactivated,recombinant bovine herpesvirus type 5 (BoHV-5) vaccine[J].Vet Microbiol,2011,148:18-26.
[8] Sarangi L N,Naveena T,Rana S K,et al.Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR[J].J Virol Methods,2018,257:1-6
[9] Raaperi K,Orro T,Viltrop A.Epidemiology and control of bovine herpesvirus 1 infection in Europe[J].Vet J,2014,201(3):249-256.
[10] 周跃辉.牛传染性鼻气管炎病毒糖蛋白gD单抗制备及其抗原表位鉴定与双抗夹心ELISA的建立[D].黑龙江哈尔滨:哈尔滨兽医研究所,2015.
[11] Dummer L A,Leite F P L.Bovine herpesvirus glycoprotein D:a review of its structural characteristics and applications in vaccinology[J].Vet Res,2014,45:111.
[12] 宋先荣.BoHV-5 mir-B10-3p诱饵系统的建立与应用[D].湖北武汉:华中农业大学,2016.
[13] Gabev E,Tobler K,Abril C,et al.Glycoprotein D of bovine herpesvirus 5(BoHV-5) confers an extended host range to BoHV-1 but does not contribute to invasion of the brain[J].J Virol,2010,84:5583-5593.
[14] Newcomer B W,Cofield L G,Walz P H,et al.Prevention of abortion in cattle following vaccination against bovine herpesvirus 1:A meta-analysis[J].Prev Vet Med,2017,138:1-8.
[15] Siedler B S,Roloff B C,de Sá G L,et al.Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle[J].Protein Expr Purif,2017,130:21-27.
[16] Silvestro C,Bratanich A.The latency related gene of bovine herpesvirus types 1 and 5 and its modulation of cellular processes[J].Arch Virol,2016,161(12):3299-3308.
[17] Chase C C L,Fulton R W,O'Toole D,et al.Bovine herpesvirus 1 modified live virus vaccines for cattle reproduction:Balancing protection with undesired effects[J].Vet Microbiol,2017,206:69-77.
[18] 冷雪,郭利,张淑琴,等.牛传染性鼻气管炎活疫苗安全性和免疫保护效果研究[J].中国畜牧兽医,2011,38(10):181-184.
[19] Zhu L,Thompson J,Ma F,et al.Effects of the synthetic corticosteroid dexamethasone on bovine herpesvirus 1 productive infection[J].Virology,2017,505:71-79.
[20] 霍志云,胡嘉欣,童钦,等.Ⅰ型牛疱疹病毒潜伏感染机制研究进展[J].中国人兽共患病学报,2012,28(1):70-73.
[21] 刘瑞宁.牛传染性鼻气管炎疫苗研究进展[J].动物医学进展,2016,37(2):85-90.
[22] Shahin F,Raza S,Yang K,et al.Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading[J].Oncotarget,2017,8(55):94462-94480.
[23] Van Drunen Littel-van den Hurk,Lawman Z,Wilson D,et al.Electroporation enhances immune responses and protection induced by a bovine viral diarrhea virus DNA vaccine in newborn calves with maternal antibodies [J].Vaccine,2010,28:6445-6454.
[24] Skoberne M,Cardin R,Lee A,et al.An adjuvanted herpes simplex virus 2 subunit vaccine elicits a T cell response in mice and is an effective therapeutic vaccine in Guinea pigs[J].J Virol,2013,87:3930-3942.
[25] Ayalew L E,Kumar P,Gaba A,et al.Bovine adenovirus-3 as a vaccine delivery vehicle[J].Vaccine,2015,33(4):493-499.
[26] Tuncer-G?ktuna P,Alpay G,?ner E B,et al.The role of herpesviruses (BoHV-1 and BoHV-4) and pestiviruses (BVDV and BDV) in ruminant abortion cases in western Turkey[J].Trop Anim Health Prod,2016,48(5):1021-1027.
[27] Rosamilia A,Jacca S,Tebaldi G,et al.BoHV-4-based vector delivering Ebola virus surface glycoprotein[J].J Transl Med,2016,14(1):325.
[28] Lima L R,Silva A P,Schmidt-Chanasit J,et al.Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2):use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction[J].Mem Inst Oswaldo Cruz,2017,112(3):220-223.