不同燕麦种质萌发期耐盐性评价
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摘要
设置4个NaCl浓度梯度(0、0.3%、0.6%、0.9%),对12份燕麦种质进行萌发期耐盐性研究和评价,以期筛选出耐盐性较强的燕麦种质材料。结果表明:不同的燕麦种质材料对盐胁迫的耐受能力不同,低浓度(0.3%)胁迫对青永久30、424、110的萌发、相对根长和相对芽长有一定的促进作用,随着盐浓度的升高,大部分供试材料的发芽势、发芽率、相对芽长以及相对根长受到了不同程度的抑制。通过五级综合评价法,从12份材料中筛选出了5个耐盐性较强的材料,分别为青永久195、青永久30、白燕7号、126和474。进一步对这5份材料在0.9%盐胁迫下的水分利用效率、叶绿素含量、根、叶鲜重进行了测定,与其他材料相比,青永久195的水分利用效率、根、叶鲜重最高。最终筛选出耐盐性最强的燕麦材料为青永久195,为下一步挖掘其耐盐基因奠定了基础。
The aim fo the current study is to screen out oat germplasm with strong salt tolerance.This study used four different NaCl concentrations(0,0.3%,0.6%,0.9%) on 12 different oat germplasm to evaluate their salt tolerance during germination.The results showed that different oat materials had different tolerance to salt stress.Low concentration(0.3%) stress promoted the germination,root length and shoot length.High concentration seriously inhibited oat germination and seedlings growth.Five materials with strong salt tolerance were selected from 12 materials,namely Qing yongjiu 195,Qing yongjiu 30,Baiyan 7,126 and 474.The water use efficiency,chlorophyll content,root and leaf fresh weight of the five materials under salt stress were furtherly examined,and the most salt-tolerant oat material was selected as Qing yongjiu195,which laid a foundation for the further exploration of its salt tolerance genes.
The aim fo the current study is to screen out oat germplasm with strong salt tolerance.This study used four different NaCl concentrations(0,0.3%,0.6%,0.9%) on 12 different oat germplasm to evaluate their salt tolerance during germination.The results showed that different oat materials had different tolerance to salt stress.Low concentration(0.3%) stress promoted the germination,root length and shoot length.High concentration seriously inhibited oat germination and seedlings growth.Five materials with strong salt tolerance were selected from 12 materials,namely Qing yongjiu 195,Qing yongjiu 30,Baiyan 7,126 and 474.The water use efficiency,chlorophyll content,root and leaf fresh weight of the five materials under salt stress were furtherly examined,and the most salt-tolerant oat material was selected as Qing yongjiu195,which laid a foundation for the further exploration of its salt tolerance genes.
引文
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[23] 蔺军.黑麦和燕麦种子萌发期耐盐性、抗旱性比较[J].草地与牧草,2016,36(4):31-34.
[2] 吕昕培,张吉平,李永生,等.内蒙古科尔沁草原不同植物生境土壤盐分特征研究[J].草地学报,2017,25(4):749-755.
[3] 刘凤歧,刘杰淋,朱瑞芬,等.4种燕麦对NaCl胁迫的生理响应及耐盐性评价[J].草业学报,2015,24(1):183-189.
[4] 德英,石凤翎,赵来喜,等.老芒麦种质资源耐盐性评价[J].草地学报,2017,39(6):107-108.
[5] 陈新,张宗文,吴斌.裸燕麦萌发期耐盐性综合评价与耐盐种质筛选[J].中国农业科学2014,47(10):2038-2046
[6] 赵桂琴,慕平.饲用燕麦研究进展[J].草业学报,2007,16(4):116-125.
[8] 王善仙,刘宛,李培军,等.盐碱土植物改良研究进展[J].中国农学通报,2011,27(24):1-7.
[9] 赵德明.燕麦草生产利用现状及发展趋势[J].黑龙江畜牧兽医,2016(22):177-179.
[10] 李威,颜红波,王朋.4个燕麦品种萌发期耐盐能力的研究[J].青海畜牧兽医杂志,2013,43(1):8-9
[11] 时丽冉,白丽荣,吕亚慈,等.小麦杂交品种衡9966苗期耐盐性分析[J].作物杂志,2018(6):149-153.
[12] 吴文荣.玉米不同材料芽苗期抗旱性及指标的研究[D].北京:中国农业科学院研究生院,2008.
[13] 苗丽宏,袁庆华,王瑜.41个紫花苜蓿品种苗期耐盐性评价[J].种子,2016,35(4):81-84.
[14] 熊建林.盐胁迫对不同种源地燕麦种子萌发的影响研究[J].安徽农学通报,2015,21(20):21-23.
[15] 刘勇,周青平,颜红波,等.3种裸燕麦种子萌发期耐盐性的研究[J].青海大学学报,2013,31(5):53-54.
[16] 赵晓军,王守顺,李生军.30份燕麦种质材料萌发期耐盐性评价[J].黑龙江畜牧兽医,2012(19):90-92
[17] 刘国富,张宇龙,李玉龙,等.盐胁迫对5种燕麦种子萌发的影响[J].饲草与饲料,2017,10(上):150-152.
[18] 刘建新,王金成,王瑞娟,等.外源过氧化氢提高燕麦耐盐性的生理机制[J].草业学报,2016,25(2):216-222
[19] 郭伟.盐碱胁迫对小麦生长的影响及腐殖酸调控效应[D].沈阳:沈阳农业大学,2011.
[20] 孙永媛.小麦耐盐生理及耐盐相关基因TaNHX3功能的初步研究[D].保定:河北农业大学,2011.
[21] 郭鹏,李琳琳,金华等.盐胁迫下紫花苜蓿突变体形态结构特征和水分利用效率的研究[J].大连民族大学学报,2016,18(3),196-197.
[22] 罗志娜,赵桂琴,刘欢.24个燕麦材料种子萌发耐盐性综合评价[J].草原与草坪,2012,32(1):38-41.
[23] 蔺军.黑麦和燕麦种子萌发期耐盐性、抗旱性比较[J].草地与牧草,2016,36(4):31-34.