牛樟芝发酵液提取物抗耐药性细菌活性研究
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摘要
采用抑菌圈法评价牛樟芝乙酸乙酯提取物对7种多重耐药性人体致病细菌(肺炎克雷伯菌、溶血性葡萄球菌、金黄色葡萄球菌、大肠埃希菌、粪肠球菌、鲍曼不动杆菌、绿脓杆菌)的抗菌活性,并检测相应致病细菌的最低抑制质量浓度.结果表明:牛樟芝提取物对7种供试细菌均有显著的抗菌活性.对多重耐药性鲍曼不动杆菌的最低抑制质量浓度达到6. 25 mg·m L-1,而质量浓度为50. 00 mg·m L-1的6种抗生素(氯霉素、庆大霉素、氨苄青霉素、链霉素、四环素、卡那霉素)对鲍曼不动杆菌均没有抑菌效果. 50. 00 mg·m L-1的牛樟芝提取物对绿脓杆菌的抑菌效果优于相同质量浓度下庆大霉素、氨苄青霉素、氯霉素.该研究有利于新型抗耐药性细菌药物的研发.
Inoculating inhibition zone was used to evaluate the antibacterial activity against 7 test strains(Klebsiella pneumoniae,Straphylococcus haemolyticus,Staphylococcus aureus,Escherichia coli,Enterococcus faecalis,Acinetobacter baumannii,Pseudomonas aeruginosa),and minimum inhibitory concentration was determined. The extracts showed significant antimicrobial activity against 7 test strains. The MIC of A. cinnamomea extracts against multidrug-resistant bacteria A. baumannii reached 6. 25 mg·m L-1,while 6 antibiotics(Chloramphenicol,Gentamycin,Ampicillin,Streptomycin,Tetracycline,Knanmycin) had no antibacterial effect in the concentration of 50. 00 mg·m L-1.The antibacterial effect of extracts against P. aeruginosa were better than Chloramphenicol,Gentamycin,and Ampicillin,in the concentration of 50. 00 mg·m L-1. The study is beneficial to the development of new drugs against resistant human pathogenic bacteria.
Inoculating inhibition zone was used to evaluate the antibacterial activity against 7 test strains(Klebsiella pneumoniae,Straphylococcus haemolyticus,Staphylococcus aureus,Escherichia coli,Enterococcus faecalis,Acinetobacter baumannii,Pseudomonas aeruginosa),and minimum inhibitory concentration was determined. The extracts showed significant antimicrobial activity against 7 test strains. The MIC of A. cinnamomea extracts against multidrug-resistant bacteria A. baumannii reached 6. 25 mg·m L-1,while 6 antibiotics(Chloramphenicol,Gentamycin,Ampicillin,Streptomycin,Tetracycline,Knanmycin) had no antibacterial effect in the concentration of 50. 00 mg·m L-1.The antibacterial effect of extracts against P. aeruginosa were better than Chloramphenicol,Gentamycin,and Ampicillin,in the concentration of 50. 00 mg·m L-1. The study is beneficial to the development of new drugs against resistant human pathogenic bacteria.
引文
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[3]陈佰义,何礼贤,胡必杰,等.中国鲍曼不动杆菌感染诊治与防控专家共识[J].中国医药科学,2012,92(8):3-8.
[4] WANG W X,KUSARI S,LAATSCH H,et al. Antibacterial azaphilones from an endophytic fungus,Colletotrichum sp.BS4[J]. Journal of Natural Products,2016,79(4):704-710.
[5] BAI H,WU L,YANG T,et al. Isolation and identification of secondary metabolites from fungus Chaetomium gracile and their antimicrobial activities[J].应用与环境生物学报,2015,21(2):274-278.
[6]张远腾,李晓波.牛樟芝化学成分及其药理作用研究进展[J].中草药,2016,47(6):1034-1042.
[7]王丽平,陆小路,赵宗杰.中药牛樟芝研究进展[J].今日药学,2017,27(2):140-144.
[8]单丽珠,宋腾,王华庆.牛樟芝联合化疗对恶性肿瘤患者免疫功能影响[J].中国中西医结合外科杂志,2015(6):552-555.
[9]张东柱.台湾特有珍贵药用真菌牛樟芝[J].食药用菌,2011,19(1):33-34.
[10] LIEN H M,TSENG C J,HUANG C L,et al. Antimicrobial activity of antrodia camphorata extracts against oral bacteria[J]. Plo S One,2014,9(8):e105286.
[11] GEETHANGILI M,FANG S H,LAI C H,et al. Inhibitory effect of Antrodia camphorata constituents on the Helicobacter pylori-associated gastric inflammation[J]. Food Chemistry,2010,119(1):149-153.
[12]赵能,原晓龙,陈剑,等.牛樟芝发酵液提取物抗菌活性研究[J].广西植物,2017,37(8):1068-1073.
[13] CHIANG Y M,OAKLEY C E,AHUJA M,et al. An efficient system for heteroogous expression of secondary metabolite genes in Aspergillus nidulans[J]. Journal of the American Chemical Society,2013,135(20):7720-7731.
[14] FANG X,YANG B,CHENG Z,et al. Synthesis and antimicrobial activity of novel chalcone derivatives[J]. Res Chem Interm,2014,40(4):1715-1725.
[15]党悦方,田娇,尚娇,等.太白山4种药用地衣抑菌活性研究[J].西北大学学报(自然科学版),2014,44(3):433-438.
[16]赵敏,谭钢文,唐小异,等.十种中药提取物与抗生素合用对铜绿假单胞菌最小抑菌质量浓度影响的体外实验[J].湖南师范大学学报(医学版),2011,8(4):80-83.
[17] MCCONNELL M J,ACTIS L,PACHN J. Acinetobacter baumannii:human infections,factors contributing to pathogenesis and animal models[J]. FEMS Microbiology Reviews,2013,37(2):130-155.
[18] SHIE P H,WANG S Y,LAY H L,et al. 4,7-dimethoxy-5-methyl-1,3-benzodioxole from Antrodia camphorata inhibits LPS-induced inflammation via suppression of NF-KB and induction HO-1 in RAW264. 7 cells[J]. International Immunopharmacology,2015,31:186-196.
[19] TU S H,WU C H,CHEN L C,et al. In vivo antitumor effects of 4,7-dimethoxy-5-methyl-1,3-benzodioxole isolated from the fruiting body of Antrodia camphorata through activation of the p53-mediated p27/Kip1 signaling pathway[J].Journal of Agricultural&Food Chemistry,2012,60(14):3612-3618.
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