卵形鲳鲹幼鱼对维生素B_2的需要量
|
摘要
为研究卵形鲳鲹(Trachinotus ovatus)幼鱼对饲料中维生素B_2的最适需要量,配制维生素B_2水平为10 mg·kg~(-1)、4.4 mg·kg~(-1)、6.2 mg·kg~(-1)、8.0 mg·kg~(-1)、11.3 mg·kg~(-1)、15.5 mg·kg~(-1)的6种实验饲料,每种饲料设3个平行,每个平行放25尾初始均质量为(11.63±0.17) g的卵形鲳鲹幼鱼,每日2次饱食,投喂8周。结果显示,4.4 mg·kg~(-1)组幼鱼的增重率、特定生长率均显著高于0 mg·kg~(-1)组(P<0.05),血清中谷草转氨酶、谷丙转氨酶活性显著低于0 mg·kg~(-1)组(P<0.05),但饲料中维生素B_2水平对饲料系数、成活率、肝脏维生素B_2含量、血清免疫指标、全鱼粗脂肪和粗蛋白、肌肉水分、粗脂肪和灰分均无显著影响(P>0.05);6.2 mg·kg~(-1)组肝脏D-氨基酸氧化酶(D-AAO)活性显著高于0 mg·kg~(-1)组(P<0.05)。折线模型分析显示,卵形鲳鲹获得最佳生长和肝脏D-AAO活力达到最大时对饲料中维生素B_2的需要量分别为4.23 mg·kg~(-1)和6.24 mg·kg~(-1)。
To determine the optimal dietary vitamin B_2 requirement of juvenile golden pompano(Trachinotus ovatus), six experimental diets were formulated which contained 0 mg·kg~(-1), 4.4 mg·kg~(-1), 6.2 mg·kg~(-1), 8.0 mg·kg~(-1), 11.3 mg·kg~(-1) and 15.5 mg·kg~(-1) of vitamin B_2. Each diet was assigned to three parallels of 25 fish [initial mass of(11.63±0.17) g] which were fed twice daily for eight weeks. The results show that the weight gain rate and specific growth rate of the fish fed with diet of 4.4 mg·kg~(-1) vitamin B_2 were significantly higher than those of 0 mg·kg~(-1) group(P<0.05), and the activities of aspartate aminotransferase and alanine aminotrans-ferase in the serum were significantly lower than those in 0 mg·kg~(-1) group(P<0.05). No significant effect was found on the fish coefficient, survival rate, liver vitamin B_2 content, serum immune parameters, crude protein and crude fat content in whole body, moisture, crude fat and crude ash in muscle(P>0.05); the liver D-AAO activity in 6.2 mg·kg~(-1) group was significantly higher in 0 mg·kg~(-1) group(P<0.05). Broken-line model analysis estimates that the dietary vitamin B_2 requirement of juvenile golden pompano is 4.23 mg·kg~(-1) for optimal growth, and 6.24 mg·kg~(-1) for maximum liver D-AAO activity.
To determine the optimal dietary vitamin B_2 requirement of juvenile golden pompano(Trachinotus ovatus), six experimental diets were formulated which contained 0 mg·kg~(-1), 4.4 mg·kg~(-1), 6.2 mg·kg~(-1), 8.0 mg·kg~(-1), 11.3 mg·kg~(-1) and 15.5 mg·kg~(-1) of vitamin B_2. Each diet was assigned to three parallels of 25 fish [initial mass of(11.63±0.17) g] which were fed twice daily for eight weeks. The results show that the weight gain rate and specific growth rate of the fish fed with diet of 4.4 mg·kg~(-1) vitamin B_2 were significantly higher than those of 0 mg·kg~(-1) group(P<0.05), and the activities of aspartate aminotransferase and alanine aminotrans-ferase in the serum were significantly lower than those in 0 mg·kg~(-1) group(P<0.05). No significant effect was found on the fish coefficient, survival rate, liver vitamin B_2 content, serum immune parameters, crude protein and crude fat content in whole body, moisture, crude fat and crude ash in muscle(P>0.05); the liver D-AAO activity in 6.2 mg·kg~(-1) group was significantly higher in 0 mg·kg~(-1) group(P<0.05). Broken-line model analysis estimates that the dietary vitamin B_2 requirement of juvenile golden pompano is 4.23 mg·kg~(-1) for optimal growth, and 6.24 mg·kg~(-1) for maximum liver D-AAO activity.
引文
[1]项昭保,戴传云,朱蠡庆.核黄素生理生化特征及其功能[J].食品研究与开发,2004, 25(6):90-92.
[2]GOLBACH J L,RICKE S C,O'BRYAN C A, et al. Riboflavin in nutrition, food processing, and analysis:a review[J]. J Food Res,2014, 3(6):23-35.
[3]荻野珍吉,陈国铭,黄小秋.鱼类的营养和饲料[M].北京:海洋出版社,1987:13-34.
[4]BARILE M, GIANCASPERO T A, LEONE P, et al. Riboflavin transport and metabolism in humans[J]. J Inherit Metab Dis, 2016,39(4):545-557.
[5]JOBLING M. National Research Council(NRC):nutrient requirements of fish and shrimp[J]. Aquacult Int, 2012, 20(3):601-602.
[6]蒋明,任春,文华,等.吉富罗非鱼对饲料中维生素B2的需要量[J].动物营养学报,2017, 29(11):3962-3969.
[7]姜建湖,陈建明,沈斌乾,等.草鱼幼鱼对饲料中核黄素的需要量[J].动物营养学报,2016, 28(9):2771-2777.
[8]张春晓.大黄鱼、鲈鱼主要B族维生素和矿物质-磷的营养生理研究[D].青岛:中国海洋大学,2006:45-56.
[9]SHIMENO S. Yellowtail, Seriola quinqueradiata[M]//WILSON R P. Handbook of nutrient requirement of finfish. Boca Raton:CRC Press, 1991:181-191.
[10]王锦林.异育银鲫对维生素B2、维生素B6和烟酸的需求量的研究[D].武汉:中国科学院研究生院(水生生物研究所),2007:38-44.
[11]王菲,李向飞,李鹏飞,等.饲料维生素B2水平对团头鲂幼鱼生长性能、体组成、抗氧化功能和肠道酶活性的影响[J].江苏农业科学,2016, 44(5):319-324.
[12]DENG D F, WILSON R P. Dietary riboflavin requirement of juvenile sunshine bass(Morone chrysops♀×Morone saxatilis(?))[J]. Aquaculture, 2003, 218(1/2/3/4):695-701.
[13]MURAI T, ANDREWS J W. Riboflavin requirement of channel catfish fingerlings[J].J Nutr, 1978, 108(9):1512-1517.
[14]SERRINI G, ZHANG Z, WILSON R P. Dietary riboflavin requirement of fingerling channel catfish(Ictalurus punctatus)[J].Aquaculture, 1996, 139(3):285-290.
[15]TAKEUCHIL, TAKEUCHI T, OGINO C. Riboflavin requirements in carp and rainbow trout[J]. Nippon Suisan Gakk, 1980,46(6):733-737.
[16]AMEZAGA M R, KNOX D. Riboflavin requirements in ongrowing rainbow trout, Oncorhynchus mykiss[J]. Aquaculture,1990, 88(1):87-98.
[17]李伟.核黄素对幼建鲤生长及消化吸收功能的影响[D].四川:四川农业大学,2008:34-46.
[18]唐媛媛,张蕉南,艾春香,等.卵形鲳鰺的营养需求研究及其配合饲料研发[J].饲料工业,2013, 34(8):46-50.
[19]杜强.卵形鲳鰺赖氨酸和蛋氨酸需求量及饲料中鱼粉替代的研究[D].上海:上海海洋大学,2012:12.
[20]刘安龙.草鱼幼鱼对饲料中核黄素、生物素和泛酸需要量的研究[D].武汉:华中农业大学,2007:18-19.
[21]顾清,周朋辉,张静姝,等.核黄素对高脂血症大鼠脂质代谢的影响[J].中国慢性病预防与控制,2015,23(1):34-36.
[22]CASILLAS E, SUNDQUIST J, AMES W E. Optimization of assay conditions for, and the selected tissue distribution of, alanine aminotransferase and aspartate aminotransferase of English sole,Parophrys vetulus Girard[J]. J Fish Biol, 1982, 21(2):197-204.
[23]黄凯,施志仪,李文娟,等.三角帆蚌内脏团不同插核部位对机体生理代谢的影响[J].水生生物学报,2013, 37(6):1085-1093.
[24]SANCHES S C, RAMALHO L N, MENDESBRAZ M, et al. Riboflavin(vitamin B-2)reduces hepatocellular injury following liver ischaemia and reperfusion in mice[J]. Food Chem Toxicol,2014, 67(5):65-71.
[25]赵书燕,林黑着,黄忠,等.不同蛋白质水平下添加小肽对石斑鱼生长、消化酶、血清生化和抗氧化能力的影响[J].南方水产科学,2016, 12(3):15-23.
[26]麦康森.水产动物营养与饲料学[M].北京:中国农业出版社,2011:242-252.
[27]NIU H, JIA Y, HU P, et al. Effect of dietary vitamin E on the growth performance and nonspecific immunity in sub-adult turbot(Scophthalmus maximus)[J]. Fish Shellfish Immun, 2014,41(2):501-506.
[28]HALVER J E, HARDY R W. Nutrient flow and retention[M]//HALVER J E, HARDY R W. Fish nutrition. San Diego,Academic Press, 2002:755-770.
[29]WOODWARD B. Sensitivity of hepatic D-amino acid oxidase and glutathione reductase to the riboflavin status of the rainbow trout(Salmogairdneri)[J]. Aquaculture, 1983,34(3/4):193-201.
[30]WOODWARD B, FRIGG M. Dietary biotin requirements of young rainbow trout(Salmo gairdneri)determined by weight gain, hepatic biotin concentration and maximal biotin-dependent enzyme activities in liver and white muscle[J]. J Nutr, 1989,119(1):54-60.
[2]GOLBACH J L,RICKE S C,O'BRYAN C A, et al. Riboflavin in nutrition, food processing, and analysis:a review[J]. J Food Res,2014, 3(6):23-35.
[3]荻野珍吉,陈国铭,黄小秋.鱼类的营养和饲料[M].北京:海洋出版社,1987:13-34.
[4]BARILE M, GIANCASPERO T A, LEONE P, et al. Riboflavin transport and metabolism in humans[J]. J Inherit Metab Dis, 2016,39(4):545-557.
[5]JOBLING M. National Research Council(NRC):nutrient requirements of fish and shrimp[J]. Aquacult Int, 2012, 20(3):601-602.
[6]蒋明,任春,文华,等.吉富罗非鱼对饲料中维生素B2的需要量[J].动物营养学报,2017, 29(11):3962-3969.
[7]姜建湖,陈建明,沈斌乾,等.草鱼幼鱼对饲料中核黄素的需要量[J].动物营养学报,2016, 28(9):2771-2777.
[8]张春晓.大黄鱼、鲈鱼主要B族维生素和矿物质-磷的营养生理研究[D].青岛:中国海洋大学,2006:45-56.
[9]SHIMENO S. Yellowtail, Seriola quinqueradiata[M]//WILSON R P. Handbook of nutrient requirement of finfish. Boca Raton:CRC Press, 1991:181-191.
[10]王锦林.异育银鲫对维生素B2、维生素B6和烟酸的需求量的研究[D].武汉:中国科学院研究生院(水生生物研究所),2007:38-44.
[11]王菲,李向飞,李鹏飞,等.饲料维生素B2水平对团头鲂幼鱼生长性能、体组成、抗氧化功能和肠道酶活性的影响[J].江苏农业科学,2016, 44(5):319-324.
[12]DENG D F, WILSON R P. Dietary riboflavin requirement of juvenile sunshine bass(Morone chrysops♀×Morone saxatilis(?))[J]. Aquaculture, 2003, 218(1/2/3/4):695-701.
[13]MURAI T, ANDREWS J W. Riboflavin requirement of channel catfish fingerlings[J].J Nutr, 1978, 108(9):1512-1517.
[14]SERRINI G, ZHANG Z, WILSON R P. Dietary riboflavin requirement of fingerling channel catfish(Ictalurus punctatus)[J].Aquaculture, 1996, 139(3):285-290.
[15]TAKEUCHIL, TAKEUCHI T, OGINO C. Riboflavin requirements in carp and rainbow trout[J]. Nippon Suisan Gakk, 1980,46(6):733-737.
[16]AMEZAGA M R, KNOX D. Riboflavin requirements in ongrowing rainbow trout, Oncorhynchus mykiss[J]. Aquaculture,1990, 88(1):87-98.
[17]李伟.核黄素对幼建鲤生长及消化吸收功能的影响[D].四川:四川农业大学,2008:34-46.
[18]唐媛媛,张蕉南,艾春香,等.卵形鲳鰺的营养需求研究及其配合饲料研发[J].饲料工业,2013, 34(8):46-50.
[19]杜强.卵形鲳鰺赖氨酸和蛋氨酸需求量及饲料中鱼粉替代的研究[D].上海:上海海洋大学,2012:12.
[20]刘安龙.草鱼幼鱼对饲料中核黄素、生物素和泛酸需要量的研究[D].武汉:华中农业大学,2007:18-19.
[21]顾清,周朋辉,张静姝,等.核黄素对高脂血症大鼠脂质代谢的影响[J].中国慢性病预防与控制,2015,23(1):34-36.
[22]CASILLAS E, SUNDQUIST J, AMES W E. Optimization of assay conditions for, and the selected tissue distribution of, alanine aminotransferase and aspartate aminotransferase of English sole,Parophrys vetulus Girard[J]. J Fish Biol, 1982, 21(2):197-204.
[23]黄凯,施志仪,李文娟,等.三角帆蚌内脏团不同插核部位对机体生理代谢的影响[J].水生生物学报,2013, 37(6):1085-1093.
[24]SANCHES S C, RAMALHO L N, MENDESBRAZ M, et al. Riboflavin(vitamin B-2)reduces hepatocellular injury following liver ischaemia and reperfusion in mice[J]. Food Chem Toxicol,2014, 67(5):65-71.
[25]赵书燕,林黑着,黄忠,等.不同蛋白质水平下添加小肽对石斑鱼生长、消化酶、血清生化和抗氧化能力的影响[J].南方水产科学,2016, 12(3):15-23.
[26]麦康森.水产动物营养与饲料学[M].北京:中国农业出版社,2011:242-252.
[27]NIU H, JIA Y, HU P, et al. Effect of dietary vitamin E on the growth performance and nonspecific immunity in sub-adult turbot(Scophthalmus maximus)[J]. Fish Shellfish Immun, 2014,41(2):501-506.
[28]HALVER J E, HARDY R W. Nutrient flow and retention[M]//HALVER J E, HARDY R W. Fish nutrition. San Diego,Academic Press, 2002:755-770.
[29]WOODWARD B. Sensitivity of hepatic D-amino acid oxidase and glutathione reductase to the riboflavin status of the rainbow trout(Salmogairdneri)[J]. Aquaculture, 1983,34(3/4):193-201.
[30]WOODWARD B, FRIGG M. Dietary biotin requirements of young rainbow trout(Salmo gairdneri)determined by weight gain, hepatic biotin concentration and maximal biotin-dependent enzyme activities in liver and white muscle[J]. J Nutr, 1989,119(1):54-60.