高寒草甸环境硒缺乏对牦牛血液生化指标及抗氧化系统功能的影响
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摘要
为探究牦牛以骨骼肌损伤为特征的疾病的发病原因及诊断和治疗方案,本试验采用电感耦合等离子体原子发射光谱法分析土壤、牧草和动物组织矿物质元素含量,应用全自动血细胞分析仪、生化分析仪及可见光分光光度法分析血液参数、生化指标和抗氧化指标。结果显示,疾病发生区土壤和牧草硒含量均极显著低于正常区域(P<0.01);患病牦牛血液、肝脏和毛发硒含量均极显著低于健康牦牛(P<0.01)。患病牦牛血红蛋白(Hb)、红细胞数(RBC)极显著低于健康牦牛(P<0.01),血清磷酸肌酸激酶(CPK)、谷丙转氨酶(GPT)、天门冬氨酸氨基转移酶(GOT)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)均极显著高于健康牦牛(P<0.01);患病牦牛血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及游离三碘甲腺原氨酸(FT_3)和三碘甲腺原氨酸(T_3)含量均极显著低于健康牦牛(P<0.01),血清丙二醛(MDA)、游离甲状腺素(FT_4)、甲状腺素(T_4)和促甲状腺激素(TSH)含量均极显著高于健康牦牛(P<0.01)。通过注射0.1%亚硒酸钠和5%维生素E的复方灭菌溶液对患病牦牛进行治疗,治疗组牦牛血液硒含量和血清抗氧化指标逐渐恢复正常,临床症状消失。因此,推测牦牛以骨骼肌损伤为特征的疾病是土壤和牧草硒缺乏所引起,以上结果在牦牛缺硒病的诊断中具有重要意义。
In order to explore the etiology,diagnosis and treatment of the disease characterized by skeletal muscle damage in yak,mineral concentrations were determined on inductively coupled plasma atomic emission spectroscopy in soil,forage and tissue of yaks.Blood parameters,biochemical indicators and antioxidant indicators were determined on automatic hematology analyzer,biochemical analyzer and visible spectrophotometry.The results showed that concentrations of selenium in the soil and forage samples from the affected area were extremely significantly lower than those from the unaffected areas(P<0.01).The mean concentration of selenium in blood,liver and hair samples from the affected yaks were extremely significantly lower than those in unaffected yaks(P<0.01).The concentrations of Hb and RBC from the affected yaks were extremely significantly lower than those from the unaffected yaks(P<0.01).MDA,CPK,GPT,GOT,ALP,LDH,FT_4,T_4 and TSH from the affected yaks were extremely significantly higher than those from the unaffected yaks(P<0.01).SOD,CAT,GSH-Px,FT_3 and T_3 from the affected yaks were extremely significantly lower than those from the unaffected yaks(P<0.01).Treatment of affected yaks by injecting 0.1% sodium selenite and 5% vitamin E compound sterilized solution,the concentration of selenium in blood samples and blood serum antioxidant indexes from the treated group yaks were gradually returned to normal.The clinical symptoms disappeared.It indicated that yaks' muscle injury disease in this region was caused by selenium deficiency,mainly due to the low selenium content in soils and forage.The above results were important for further diagnose of yaks' selenium deficiency.
In order to explore the etiology,diagnosis and treatment of the disease characterized by skeletal muscle damage in yak,mineral concentrations were determined on inductively coupled plasma atomic emission spectroscopy in soil,forage and tissue of yaks.Blood parameters,biochemical indicators and antioxidant indicators were determined on automatic hematology analyzer,biochemical analyzer and visible spectrophotometry.The results showed that concentrations of selenium in the soil and forage samples from the affected area were extremely significantly lower than those from the unaffected areas(P<0.01).The mean concentration of selenium in blood,liver and hair samples from the affected yaks were extremely significantly lower than those in unaffected yaks(P<0.01).The concentrations of Hb and RBC from the affected yaks were extremely significantly lower than those from the unaffected yaks(P<0.01).MDA,CPK,GPT,GOT,ALP,LDH,FT_4,T_4 and TSH from the affected yaks were extremely significantly higher than those from the unaffected yaks(P<0.01).SOD,CAT,GSH-Px,FT_3 and T_3 from the affected yaks were extremely significantly lower than those from the unaffected yaks(P<0.01).Treatment of affected yaks by injecting 0.1% sodium selenite and 5% vitamin E compound sterilized solution,the concentration of selenium in blood samples and blood serum antioxidant indexes from the treated group yaks were gradually returned to normal.The clinical symptoms disappeared.It indicated that yaks' muscle injury disease in this region was caused by selenium deficiency,mainly due to the low selenium content in soils and forage.The above results were important for further diagnose of yaks' selenium deficiency.
引文
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[13] RANCHES J,VENDRAMINI J M,ARTHINGTON J D.Effects of selenium biofortification of hayfields on measures of selenium status in cows and calves consuming these forages[J].Journal of Animal Science,2017,95(1):120-128.
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[21] LEI R,JIANG N,ZHANG Q,et al.Prevalence of selenium,T-2 toxin,and deoxynivalenol in Kashin-Beck disease areas in Qinghai province,Northwest China[J].Biological Trace Element Research,2016,171(1):34-40.
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