盐胁迫下外源钙对忍冬光合系统的调控
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摘要
外源钙可以加强某些植物对非生物胁迫的抗性。然而,钙对盐胁迫下忍冬的作用尚不清楚。该试验旨在研究外源钙对盐处理下忍冬的生物量、光合色素含量、气体交换参数和快速叶绿素荧光诱导动力学曲线产生的影响。实验选用一年生忍冬为研究材料,用4个水平盐浓度(0,50,100,150 mmol·L-1)和2个水平钙浓度(0,20 mmol·L-1)处理忍冬7 d,采用JIP-test参数,结合气体交换参数,分析外源钙是否可以提高盐胁迫下忍冬的抗性。结果显示,外源钙处理的忍冬比仅用盐处理的忍冬具有更好的光化学性能,代表了光合作用中心PSⅡ整体活性的PIABS,PItotal,表征电子传递效率的相关参数φP0,ψE0,φE0,σR,φR均显著提高。同时,气体交换参数Gs,Ci,Tr也保持在较高水平,与光合色素含量和生物量变化趋势一致。综上所述,外源钙保护了PSⅡ免于失活,促进了光合电子的传递,维持了较高的胞间CO2浓度,对盐胁迫下忍冬的生长起到了积极作用。
Exogenous calcium can enhance the resistance of certain plants to abiotic stress. However,the role of calcium insaltstressed honeysuckle is unclear. The study is aimed to investigate the effects of exogenous calcium on the biomass,chlorophyll content,gas exchange parameters and chlorophyll fluorescence of honeysuckle under salt stress. The results showed that the calcium-treated honeysuckle had better photochemical properties than the salt-stressed honeysuckle,such as PIABS,PItotal,which represents the overall activity of photosystemⅡ(PSⅡ),and related parameters for characterizing electron transport efficiency φP0,ψE0,φE0,σR,and φR are significantly improved. At the same time,the gas exchange parameters Gs,Ci,Trare also maintained at a high level. In summary,exogenous calcium protects the activity of PSⅡ,promotes the transmission of photosynthetic electrons,and maintains a high Ci,therefore enhances the resistance of honeysuckle under salt stress.
Exogenous calcium can enhance the resistance of certain plants to abiotic stress. However,the role of calcium insaltstressed honeysuckle is unclear. The study is aimed to investigate the effects of exogenous calcium on the biomass,chlorophyll content,gas exchange parameters and chlorophyll fluorescence of honeysuckle under salt stress. The results showed that the calcium-treated honeysuckle had better photochemical properties than the salt-stressed honeysuckle,such as PIABS,PItotal,which represents the overall activity of photosystemⅡ(PSⅡ),and related parameters for characterizing electron transport efficiency φP0,ψE0,φE0,σR,and φR are significantly improved. At the same time,the gas exchange parameters Gs,Ci,Trare also maintained at a high level. In summary,exogenous calcium protects the activity of PSⅡ,promotes the transmission of photosynthetic electrons,and maintains a high Ci,therefore enhances the resistance of honeysuckle under salt stress.
引文
[1]李星,李凯锋.土壤盐渍化开发利用研究进展[J].农业科技通讯,2018(6):244.
[2]蔺娟,地里拜尔·苏力坦.土壤盐渍化的研究进展[J].新疆大学学报:自然科学版,2007(3):318.
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[5]刘玉峰,李鲁盼,马海燕,等.金银花化学成分及药理作用的研究进展[J].辽宁大学学报:自然科学版,2018,45(3):255.
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[10]李娟,林位夫,周立军.不同光照度生境对海芋块茎形态、淀粉含量及叶绿素含量的影响[J].华南农业大学学报,2016,37(3):62.
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[12] Yan K,Wu C,Zhang L,et al. Contrasting photosynthesis and photoinhibition in tetraploid and its autodiploid honeysuckle(Lonicera japonica Thunb.)under salt stress[J]. Front Plant Sci,2015,6:227.
[13]许卉,赵丽萍.盐胁迫对金银花生理生化的影响[J].湖北林业科技,2007(1):9.
[14]谢东锋.金银花对土壤干旱-复水的光合生理响应[D].泰安:山东农业大学,2017.
[15]李鹏民.快速叶绿素荧光诱导动力学在植物逆境生理研究中的应用[D].泰安:山东农业大学,2007.
[16]徐臣善.钙对盐胁迫下小金海棠幼苗生物量及抗氧化系统的影响[J].植物生理学报,2014,50(6):817.
[17]刘小龙,李霞,钱宝云,等.植物体内钙信号及其在调节干旱胁迫中的作用[J].西北植物学报,2014,34(9):1927.
[18]张晶晶,李金金,年洪娟.钙/钙调素信号途径在胁迫中的作用研究进展[J].中国微生态学杂志,2013,25(7):858.
[19]张振兴,孙锦,郭世荣,等.钙对盐胁迫下西瓜光合特性和果实品质的影响[J].园艺学报,2011,38(10):1929.
[20]朱晓军,杨劲松,梁永超,等.盐胁迫下钙对水稻幼苗光合作用及相关生理特性的影响[J].中国农业科学,2004(10):1497.
[21]束胜,郭世荣,孙锦,等.盐胁迫下植物光合作用的研究进展[J].中国蔬菜,2012(18):53.
[22]许大全.光合作用气孔限制分析中的一些问题[J].植物生理学通讯,1997(4):241.
[23]郑国琦,许兴,徐兆桢,等.盐胁迫对枸杞光合作用的气孔与非气孔限制[J].西北植物学报,2002(6):75.
[24] Dobrikova A G,Ivanova P I. Relationship between the organization of the PSⅡsuper complex and the functions of the photosynthetic apparatus[J]. J Photoch Photobio B,2006,83(2):114.
[25] Strasser B J. Donor side capacity of photosystemⅡprobed by chlorophyll a fluorescence transients[J]. Photosynth Res,1997,52(2):147.
[26] Wang G,Bi A,Amombo E,et al. Exogenous calcium enhances the photosystemⅡphotochemistry response in salt stressed tall fescue[J]. Front Plant Sci,2017,8:203.
[2]蔺娟,地里拜尔·苏力坦.土壤盐渍化的研究进展[J].新疆大学学报:自然科学版,2007(3):318.
[3]单奇华,张建锋,阮伟建,等.滨海盐碱地土壤质量指标对生态改良的响应[J].生态学报,2011,31(20):6072.
[4]徐臣善.钙对盐胁迫下小金海棠幼苗生物量及抗氧化系统的影响[J].植物生理学报,2014,50(6):817.
[5]刘玉峰,李鲁盼,马海燕,等.金银花化学成分及药理作用的研究进展[J].辽宁大学学报:自然科学版,2018,45(3):255.
[6]马雪梅,吴朝峰.干旱胁迫对金银花叶片叶绿素含量及荧光特性的影响[J].江苏农业科学,2018,46(17):133.
[7]董祥开,蔡悦,张雷,等.海水胁迫对金银花荧光特性的影响[J].安徽农学通报,2018,24(16):32.
[8]谢元贵,刘济明,廖小锋,等.钙胁迫对金银花生理生化特性的影响[J].江苏农业科学,2017,45(17):144.
[9]王爱玲,蔡军社,白世践,等.葡萄砧木叶片含水量和相对含水量的研究[J].天津农业科学,2013,19(2):16.
[10]李娟,林位夫,周立军.不同光照度生境对海芋块茎形态、淀粉含量及叶绿素含量的影响[J].华南农业大学学报,2016,37(3):62.
[11] Yusuf M A,Kumar D,Rajwanshi R,et al. Overexpression ofγ-tocopherol methyl transferase gene in transgenic brassica juncea plants alleviates abiotic stress:physiological and chlorophyll a fluorescence measurements[J]. BBA-Bioenergetics,2010,1797(8):1428.
[12] Yan K,Wu C,Zhang L,et al. Contrasting photosynthesis and photoinhibition in tetraploid and its autodiploid honeysuckle(Lonicera japonica Thunb.)under salt stress[J]. Front Plant Sci,2015,6:227.
[13]许卉,赵丽萍.盐胁迫对金银花生理生化的影响[J].湖北林业科技,2007(1):9.
[14]谢东锋.金银花对土壤干旱-复水的光合生理响应[D].泰安:山东农业大学,2017.
[15]李鹏民.快速叶绿素荧光诱导动力学在植物逆境生理研究中的应用[D].泰安:山东农业大学,2007.
[16]徐臣善.钙对盐胁迫下小金海棠幼苗生物量及抗氧化系统的影响[J].植物生理学报,2014,50(6):817.
[17]刘小龙,李霞,钱宝云,等.植物体内钙信号及其在调节干旱胁迫中的作用[J].西北植物学报,2014,34(9):1927.
[18]张晶晶,李金金,年洪娟.钙/钙调素信号途径在胁迫中的作用研究进展[J].中国微生态学杂志,2013,25(7):858.
[19]张振兴,孙锦,郭世荣,等.钙对盐胁迫下西瓜光合特性和果实品质的影响[J].园艺学报,2011,38(10):1929.
[20]朱晓军,杨劲松,梁永超,等.盐胁迫下钙对水稻幼苗光合作用及相关生理特性的影响[J].中国农业科学,2004(10):1497.
[21]束胜,郭世荣,孙锦,等.盐胁迫下植物光合作用的研究进展[J].中国蔬菜,2012(18):53.
[22]许大全.光合作用气孔限制分析中的一些问题[J].植物生理学通讯,1997(4):241.
[23]郑国琦,许兴,徐兆桢,等.盐胁迫对枸杞光合作用的气孔与非气孔限制[J].西北植物学报,2002(6):75.
[24] Dobrikova A G,Ivanova P I. Relationship between the organization of the PSⅡsuper complex and the functions of the photosynthetic apparatus[J]. J Photoch Photobio B,2006,83(2):114.
[25] Strasser B J. Donor side capacity of photosystemⅡprobed by chlorophyll a fluorescence transients[J]. Photosynth Res,1997,52(2):147.
[26] Wang G,Bi A,Amombo E,et al. Exogenous calcium enhances the photosystemⅡphotochemistry response in salt stressed tall fescue[J]. Front Plant Sci,2017,8:203.