基于农艺性状的甘蓝型油菜三系骨干亲本多样性分析
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摘要
为了合理利用油菜种质资源,降低组配油菜杂交组合的盲目性,对30份油菜骨干亲本材料的主要农艺性状和品质性状进行考察,采用11个农艺性状指标(株高、千粒质量、含油量等)进行遗传多样性分析。结果表明:聚类分析及主成分分析结果一致性较高,30份参试材料被分为3大类,大部分保持系和恢复系材料被分开;方差分析显示,保持系和恢复系材料内变异系数为86.40%,保持系和恢复系材料间仅为13.60%,保持系和恢复系材料内部差异大于它们之间的差异。研究结果为油菜杂交育种选配高产亲本组合和生产利用等提供重要的参考依据。
Rapeseed germplasm resources are very important for breeding in plants. In order to make efficient use of rapeseed germplasm resources and reduce blindness in combination selection of crosses, the genetic diversity of 30 elite lines in Brassica napus L.was evaluated in the study by 11 agronomic and quality traits, including plant height, thousand seeds mass, oil content and so on. The results of cluster analysis and principal component analysis were consistent. 30 tested materials were classified into three groups, most maintainer lines and restorer lines were separated each other. The variance analysis showed that the coefficient of variation within populations was 86.40%, the difference among populations was 13.60%, this showed that the difference within maintainers and restorers was greater. The present study is helpful to guide the selection of strong heterosis combinations and make more effective use of heterosis.
Rapeseed germplasm resources are very important for breeding in plants. In order to make efficient use of rapeseed germplasm resources and reduce blindness in combination selection of crosses, the genetic diversity of 30 elite lines in Brassica napus L.was evaluated in the study by 11 agronomic and quality traits, including plant height, thousand seeds mass, oil content and so on. The results of cluster analysis and principal component analysis were consistent. 30 tested materials were classified into three groups, most maintainer lines and restorer lines were separated each other. The variance analysis showed that the coefficient of variation within populations was 86.40%, the difference among populations was 13.60%, this showed that the difference within maintainers and restorers was greater. The present study is helpful to guide the selection of strong heterosis combinations and make more effective use of heterosis.
引文
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[21] TIAN H Y,LI W,VRATISLAV K,et al.Phenotypic diversity of rapeseed accessions from different geographic locations [J].Oil Crop Science,2016,1:9-20.
[22] 高必军,李军,洪江.甘蓝型油菜若干农艺性状与单株产量的关系分析[J].生物数学学报,2017,22(1):137-144. GAO B J,LI J,HONG J.Relation analysis of several agronomic traits and yield per plant in Brassica napus L.[J].Journal of Biomathematics,2017,22(1):137-144.
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[24] 蒲晓斌,王茂林,栾丽,等.中国西南地区芥菜型油菜资源遗传多样性分析[J].中国农业科学,2007,40(8):1610-1621. PU X B,WANG M L,LUAN L,et al.Genetic diversity analysis of Brassica juncea L.and races in Southwest China [J].Scientia Agricultura Sinica,2007,40(8):1610-1621.
[25] ANA M,ANKICA K,DEJANA S,et al.Phenotypic and molecular evaluation of genetic diversity of rapeseed(Brassica napus L.) genotypes [J].African Journal of Biotechnology,2009,8(19):4835-4844.
[26] 臧珊.甘蓝型油菜三系骨干亲本遗传多样性分析[D].陕西杨凌:西北农林科技大学,2018. ZANG SH.Genetic diversity of a panel of elite parental lines in Brassica napus L.[D].Yangling Shannxi:Northwest A&F University,2018.
[27] QIAN W,SASS O,MENG J,et al.Heterotic patterns in rapeseed(Brassica napus L.):I.Crosses between spring and Chinese semi-winter lines [J].Theoretical and Applied Genetics,2007,115(1):27-34.
[28] QIAN W,LI Q,NOACK J,et al.Heterotic patterns in rapeseed(Brassica napus L.):Ⅱ.Crosses between European winter and Chinese semi-winter lines [J].Plant Breeding,2009,128(5):466-470.
[29] TIAN H Y,CHANNA S A,HU S W.Heterotic grouping and the heterotic pattern among Chinese rapeseed accessions(Brassica napus L.) [J].Agronomy Journal,2015,107(4):1321-1330.
[2] CHALHOUB B,DENOEUD F,LIU S,et al.Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome [J].Science,2014,345(6199):950-953.
[3] 傅廷栋.杂交油菜的育种与利用[M].武汉:湖北科学技术出版社,2000:6-22. FU T D.Breeding and Utilization of Hybrid Rape [M].Wuhan:Hubei Science & Technology Press,2000:6-22.
[4] ALI M,COPELAND L O,ELIAS S G,et al.Relationship between genetic distance and heterosis for yield and morphological traits in winter canola(Brassica napus L.) [J].Theoretical and Applied Genetics,1995,91(1):118-121.
[5] 叶威.甘蓝型油菜种质资源遗传多样性研究[D].武汉:华中农业大学,2011. YE W.Studies on genetic diversity of Brassica napus germplasm resources [D].Wuhan:Huazhong Agricultural University,2011.
[6] HOREJSI J,STAUB J E.Genetic variation in cucumber(Cucumis sativus L.) as assessed by random amplified polymorphic DNA [J].Genetic Resources and Crop Evolution,1999,46:337-350.
[7] GEPTS P,PAPA R.Possible effects of(trans)gene flow from crops on the genetic diversity from landraces and wild relatives [J].Environmental Biosafety Research,2003,2:89-103.
[8] BECHERE E,BELAY D,MITIKU D,et al.Phenotypic diversity of tetraploid wheat landraces from northern and north-central regions of Ethiopia [J].Hereditas,1996,124:165-172.
[9] ALLEMAYEHU A,MARYKE T.Phenotypic variation in barley(Hordeum vulgare L.) landraces from north Shewa in Ethiopia [J].Biodiversity and Conservation,2004,13:1441-1451.
[10] TERZOPOULOS P J,BEBELI P J.Phenotypic diversity in Greek tomato(Solanum lycopersicum L.) landraces [J].Scientia Horticulturae,2010,126:138-144.
[11] 李瑞,肖斌,宋红霞,等.50份茶树种质资源农艺性状及其聚类分析[J].西北农业学报,2011,20(10):107-111. LI R,XIAO B,SONG H X,et al.Agronomic traits and cluster analysis of 50 tea germplasm resources [J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(10):107-111.
[12] 陈卫江,李墓,于德日.杂交油菜亲本遗传距离分析与杂种优势[J].作物研究,1997(3):21-24. CHEN W J,LI M,YU D R.Parental genetic distance analysis and heterosis of hybrid rape [J].Crop Research,1997(3):21-24.
[13] HU S W,YU C Y,ZHAO H X,et al.Genetic diversity of Brassica napus L.germplasm from China and Europe assessed by some agronomically mportant characters [J].Euphytica,2007,154:9-16.
[14] K?RBER N,WITTKOP B,BUS A,et al.Seedling development in a Brassica napus diversity set and its relationship to agronomic performance [J].Theoretical and Applied Genetics,2012,125:1275-1287.
[15] CHEN B Y,XU K,LI J,et al.Evaluation of yield and agronomic traits and their genetic variation in 488 global collections of Brassica napus L.[J].Genetic Resources and Crop Evolution,2014,61:979-999.
[16] LEONARDO V,HEIKO C BECKER.Estimating the fatty acid composition of the oil in intact-seed rapeseed(Brassica napus L.) by near-infrared reflectance spectroscopy [J].Euphytica,1998,101:221-230.
[17] 张文彤.SPSS分析基础教程[M].北京:高等教育出版社,2017:26-50. ZHANG W T.SPSS Analysis Basic[M].Beijing:Higher Education Press,2017:26-50.
[18] TANG Q Y,FENG M G.Practical Statistics and DPS Data Processing System [M].Beijing:Chinese Agricultrul Press,1997:407.
[19] 刘后利.油菜遗传育种学[M].北京:中国农业大学出版,2000:32-36. LIU H L.Rapeseed Breeding Subjects [M].Beijing:China Agriculture University Press,2000:32-36.
[20] 谢永俊,曾彪,王奇彬.外国甘蓝型油菜优异种质资源的研究与利用[J].西南农业学报,2001,14(1):51-54. XIE Y J,ZENG B,WANG Q B.Utilization and research for foreign germplasm of napus rapeseed [J].Southwest China Journal of Agricultural Sciences,2001,14(1):51-54.
[21] TIAN H Y,LI W,VRATISLAV K,et al.Phenotypic diversity of rapeseed accessions from different geographic locations [J].Oil Crop Science,2016,1:9-20.
[22] 高必军,李军,洪江.甘蓝型油菜若干农艺性状与单株产量的关系分析[J].生物数学学报,2017,22(1):137-144. GAO B J,LI J,HONG J.Relation analysis of several agronomic traits and yield per plant in Brassica napus L.[J].Journal of Biomathematics,2017,22(1):137-144.
[23] 何余堂,陈宝元,傅廷栋,等.白菜型油菜在中国的起源与进化[J].遗传学报,2003,30(11):1003-1012. HE Y T,CHEN B Y,FU T D,et al.Origins and evolution of Brassica campestris L. in China [J].Journal of Genetics and Genomics,2003,30(11):1003-1012.
[24] 蒲晓斌,王茂林,栾丽,等.中国西南地区芥菜型油菜资源遗传多样性分析[J].中国农业科学,2007,40(8):1610-1621. PU X B,WANG M L,LUAN L,et al.Genetic diversity analysis of Brassica juncea L.and races in Southwest China [J].Scientia Agricultura Sinica,2007,40(8):1610-1621.
[25] ANA M,ANKICA K,DEJANA S,et al.Phenotypic and molecular evaluation of genetic diversity of rapeseed(Brassica napus L.) genotypes [J].African Journal of Biotechnology,2009,8(19):4835-4844.
[26] 臧珊.甘蓝型油菜三系骨干亲本遗传多样性分析[D].陕西杨凌:西北农林科技大学,2018. ZANG SH.Genetic diversity of a panel of elite parental lines in Brassica napus L.[D].Yangling Shannxi:Northwest A&F University,2018.
[27] QIAN W,SASS O,MENG J,et al.Heterotic patterns in rapeseed(Brassica napus L.):I.Crosses between spring and Chinese semi-winter lines [J].Theoretical and Applied Genetics,2007,115(1):27-34.
[28] QIAN W,LI Q,NOACK J,et al.Heterotic patterns in rapeseed(Brassica napus L.):Ⅱ.Crosses between European winter and Chinese semi-winter lines [J].Plant Breeding,2009,128(5):466-470.
[29] TIAN H Y,CHANNA S A,HU S W.Heterotic grouping and the heterotic pattern among Chinese rapeseed accessions(Brassica napus L.) [J].Agronomy Journal,2015,107(4):1321-1330.