应用SERS滤纸基底快速检测朱砂中违禁染料的研究
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摘要
本文通过将银纳米颗粒组装于滤纸作为SERS信号增强基底,对朱砂中违禁染料808猩红进行了快速检测。利用密度泛函理论计算了808猩红的理论拉曼光谱,并结合实测拉曼光谱,对808猩红的拉曼特征峰进行谱峰归属。通过将银纳米颗粒利用液-液界面自组装技术组装于滤纸上,制备得到SERS滤纸基底,并测试了基底的信号重复性。利用SERS滤纸基底对朱砂中808猩红进行检测,实验结果显示808猩红的最低检测限为0. 05!g/m L,在0. 05～1!g/m L浓度范围内,808猩红的浓度与其SERS强度呈线性关系,线性相关系数为R2=0. 98673。该方法简便、快速,可用于对药材中违禁染料的现场检测。
Surface-enhanced Raman spectroscopy(SERS) technology was performed by employing a paper-based SERS substrates for rapid detection of Scarlet 808(S808),an illegal dye in cinnabar.The theoretical Raman spectrum of S808 was calculated and characteristic peaks of S808 were assigned to the corresponding vibrational modes by density functional theory. For preparing the paperbased SERS substrate,the liquid-liquid interface self-assembly technology was used to assemble the silver nanoparticle on the filter paper. The substrate showed good reproducibility in the test. S808 on cinnabar was detected by paper-based SERS substrates,obtaining the minimum detection limit of 0.05 !g/m L. In addition,under the concentration ranging from 0. 05 to 1 !g/m L,the Raman intensity of S808 SERS spectrum with its concentrations of S808 in cinnabar displayed a linear relation,with the coefficient of R2= 0. 98673. Therefore,the paper-based SERS substrates can be applied into on-site detection of the illegal dye in cinnabar in an easy and rapid manner.
Surface-enhanced Raman spectroscopy(SERS) technology was performed by employing a paper-based SERS substrates for rapid detection of Scarlet 808(S808),an illegal dye in cinnabar.The theoretical Raman spectrum of S808 was calculated and characteristic peaks of S808 were assigned to the corresponding vibrational modes by density functional theory. For preparing the paperbased SERS substrate,the liquid-liquid interface self-assembly technology was used to assemble the silver nanoparticle on the filter paper. The substrate showed good reproducibility in the test. S808 on cinnabar was detected by paper-based SERS substrates,obtaining the minimum detection limit of 0.05 !g/m L. In addition,under the concentration ranging from 0. 05 to 1 !g/m L,the Raman intensity of S808 SERS spectrum with its concentrations of S808 in cinnabar displayed a linear relation,with the coefficient of R2= 0. 98673. Therefore,the paper-based SERS substrates can be applied into on-site detection of the illegal dye in cinnabar in an easy and rapid manner.
引文
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[14] Zhang C,Han S Q,Zhao H,et al. Detection and quantification of Bucinnazine Hydrochloride injection based on SERS technology[J]. Anal Sci,2018,34(11):1249-1255.
[15]欧阳爱国，张宇，唐天义等．基于拉曼光谱的乙醇柴油密度、粘度和乙醇含量分析研究光谱学与光谱分析[J]. 2018,38(6):1772-1778.(OUYANG Aiguo,ZHANG Yu,TANG Tianyi,et al. Study on density,viscosity and ethanol content of ethanol diesel based on Raman spectroscopy[J]. Spectrosc Spect Anal,2018,38(6):1772-1778)
[16] Lin S,Hasi W L J,Lin X,et al. Rapid and sensitive SERS method for determination of Rhodamine B in chili powder with paper-based substrates[J]. Anal Methods,2015,7(12):5289-5294.
[2] Xia F B,Li A,Chai Y S,et al. UPLC/Q-TOFMS-based metabolomics approach to reveal the protective role of other herbs in An-Gong-Niu-Huang Wan against the hepatorenal toxicity of cinnabar and realgar[J]. Front Pharmacol,2018,9:15.
[3] Wang H,Bai J,Chen G,et al. A metabolic profiling analysis of the acute hepatotoxicity and nephrotoxicity of Zhusha Anshen Wan compared with cinnabar in rats using H-1 NMR spectroscopy[J]. J Ethnopharmacol,2013,146(2):572-580.
[4]韩斯琴高娃，哈斯乌力吉，林翔，等．利用拉曼光谱技术检测中蒙药中朱砂的研究[J]．光谱学与光谱分析，2015,35(10):2773-2775.(HAN Siqingaowa,HASI Wuliji,LIN Xiang,et al. Detection of cinnabars in mongolian medicines using Raman spectroscopy[J]. Spectrosc Spect Anal,2015,35(10):2773-2775)
[5]苟琰，耿昭，张燕飞等．液质联用法快速筛查中药材及饮片中常见染色掺假物质的方法研究及数据库建立[J]．药物分析杂志，2017,37(3):461-470.(GOU Yan,GENG Zhao,ZHANG Yanfei,et al. Rapid screening method based on LC-MS for common dyeing adulterated material in traditional Chinese medicinal materials and decoction pieces[J]. Chin J Pharm Anal,2017,37(3):461-470)
[6]杨建龙，谭纹．LC-MS/MS同时测定朱砂中掺假染料猩红808和玫瑰红B的含量[J]．中国现代应用药学，2015,32(8):979-983.(YANG Jianlong,TAN Wen. Simultaneous determination of red adulterated dye Scarlet 808 and Rhodamine B in cinnabar by LCMS/MS[J]. Chin J Modern Appl Pharm,2015,32(8):979-983)
[7]张燕飞，耿昭，黄再强等．朱砂中添加色素的HPLCQ-TOF/MS分析以及TLC和HPLC检测方法的建立[J]．药物分析杂志，2017,37(5):852-861.(ZHANG Yanfei,GENG Zhao,HUANG Zaiqiang,et al. Analysis of added pigments in cinnabar by HPLC-Q-TOF/MS and establishment of determination method by TLC and HPLC[J]. Chin J Pharm Anal,2017,37(5):852-861)
[8]何轶，丁宁，王瑞忠等．冠脉宁片中化工染料检查及血竭素高氯酸盐含量测定研究[J]．中国药学杂志，2015,50(2):120-124.(HE Yi,DING Ning,WANG Ruizhong,et al. Test of chemical dyes and quantitative determination of dracorhodin perchlorate in Guanmaining tablets[J]. Chin Pharm J,2015,50(2):120-124)
[9] Zhang M L,Hasi W L J,Lin X,et al. Rapid and simple detection of pethidine hydrochloride injection using surface-enhanced Raman spectroscopy based on silver aggregates[J]. Anal Methods,2015,7(19):8241-8247.
[10] Zhao H,Hasi W,Bao L,et al. A silver self-assembled monolayer-decorated polydimethylsiloxane flexible substrate for in situ SERS detection of low-abundance molecules[J]. J Raman Spectrosc,2018,49(9):1469-1477.
[11] Kaminska A,Witkowska E,Kowalska A,et al. Highly efficient SERS-based detection of cerebrospinal fluid neopterin as a diagnostic marker of bacterial infection[J]. Anal Bioanal Chem,2016,408(16):4319-4327.
[12] Qin Y Q,Ji X H,Jing J,et al. Size control over spherical silver nanoparticles by ascorbic acid reduction[J].Colloids Surf A-Physicochem Eng Asp,2010,372(1-3):172-176.
[13] Hasi W L J,Lin X,Lou X T,et al. Chloride ion-assisted self-assembly of silver nanoparticles on filter paper as SERS substrate[J]. Appl Phys A-Mater Sci Process,2015,118(3):799-807.
[14] Zhang C,Han S Q,Zhao H,et al. Detection and quantification of Bucinnazine Hydrochloride injection based on SERS technology[J]. Anal Sci,2018,34(11):1249-1255.
[15]欧阳爱国，张宇，唐天义等．基于拉曼光谱的乙醇柴油密度、粘度和乙醇含量分析研究光谱学与光谱分析[J]. 2018,38(6):1772-1778.(OUYANG Aiguo,ZHANG Yu,TANG Tianyi,et al. Study on density,viscosity and ethanol content of ethanol diesel based on Raman spectroscopy[J]. Spectrosc Spect Anal,2018,38(6):1772-1778)
[16] Lin S,Hasi W L J,Lin X,et al. Rapid and sensitive SERS method for determination of Rhodamine B in chili powder with paper-based substrates[J]. Anal Methods,2015,7(12):5289-5294.