鲍鱼对2种多环芳烃的富集动力学研究
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摘要
为探究多环芳烃(PAHs)在海洋生物体内富集过程,选择皱纹盘鲍(Haliotis discus hannai)作为受试生物,应用半静态双箱动力学模型,分别考察了3-甲基菲和9,10-甲基蒽2种多环芳烃在皱纹盘鲍体内的富集动力学过程,通过非线性拟合获得鲍鱼对2种多环芳烃的吸收速率常数(K_1)、释放速率常数(K_2)、生物富集因子(BCF),以及平衡状态下鲍鱼体内2种多环芳烃的含量(Cmax)、生物学半衰期(t_(1/2))等动力学参数。结果表明,鲍鱼对9,10-甲基蒽富集动力学参数K_1、K_2、BCF、Cmax、t_(1/2)的平均值分别为4.9437、0.406、13.59、790.03μg·L~(-1)、3.78 d。鲍鱼对3-甲基菲富集动力学参数K_1、K_2、BCF、Cmax、t_(1/2)的平均值分别为2.3023、0.367、5.97、354.37μg·L~(-1)、3.13 d。鲍鱼在不同浓度下对3-甲基菲和9,10-甲基蒽的生物富集过程均符合双箱动力学模型。
In order to explore the enrichment of polycyclic aromatic hydrocarbons(PAHs) in abalone(Haliotis discus hannai), the semi-static two-compartment kinetic model was used to investigate the bioaccumulation kinetics of3-methylphenanthrene and 9,10-dimethylanthracene. The enrichment kinetics parameters of two kinds of PAHs in abalone, including the absorption rate constant(K_1), release rate constant(K_2), bioconcentration factor(BCF), content(Cmax) and biological half-life(t_(1/2)), were obtained by nonlinear fitting. The results showed that the average values of kinetic parameters K_1, K_2, BCF, Cmaxand t_(1/2) of abalone for 9,10-dimethylanthracene were 4.9437, 0.406,13.59, 790.03 μg·L~(-1) and 3.78 d, respectively. The average values of kinetic parameters K_1, K_2, BCF, Cmaxand t_(1/2) of abalone for 3-methylphenanthrene were 2.3023, 0.367, 5.97, 354.37 μg·L~(-1) and 3.13 d, respectively. The bioaccumulation process of 3-methylphenanthrene and 9,10-dimethylanthracene in different concentrations of abalone conform to the two-compartment kinetic model.
In order to explore the enrichment of polycyclic aromatic hydrocarbons(PAHs) in abalone(Haliotis discus hannai), the semi-static two-compartment kinetic model was used to investigate the bioaccumulation kinetics of3-methylphenanthrene and 9,10-dimethylanthracene. The enrichment kinetics parameters of two kinds of PAHs in abalone, including the absorption rate constant(K_1), release rate constant(K_2), bioconcentration factor(BCF), content(Cmax) and biological half-life(t_(1/2)), were obtained by nonlinear fitting. The results showed that the average values of kinetic parameters K_1, K_2, BCF, Cmaxand t_(1/2) of abalone for 9,10-dimethylanthracene were 4.9437, 0.406,13.59, 790.03 μg·L~(-1) and 3.78 d, respectively. The average values of kinetic parameters K_1, K_2, BCF, Cmaxand t_(1/2) of abalone for 3-methylphenanthrene were 2.3023, 0.367, 5.97, 354.37 μg·L~(-1) and 3.13 d, respectively. The bioaccumulation process of 3-methylphenanthrene and 9,10-dimethylanthracene in different concentrations of abalone conform to the two-compartment kinetic model.
引文
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[2]王静.栉孔扇贝对苯并[a]芘(Benzo[a]pyrene)生物标志物筛选技术的初步研究[D].青岛:中国海洋大学,2008:14-15Wang J.The primary study on identificating biomarkers of B[a]P based on Chlamys farreri[D].Qingdao:Ocean University of China,2008:14-15(in Chinese)
[3]周海森,罗世滨,张伟杰,等.近交对中间球海胆受精率、孵化率和幼体发育的影响[J].大连海洋大学学报,2013,28(5):445-449Zhou H S,Luo S B,Zhang W J,et al.Effect of inbreeding on fertility rate,hatchability and larva development of sea urchin Strongylocentrotus intermedius[J].Journal of Dalian Ocean University,2013,28(5):445-449(in Chinese)
[4]Ke R,Xu Y,Huang S,et al.Comparison of the uptake of polycyclic aromatic hydrocarbons and organochlorine pesticides by semipermeable membrane devices and caged fish(Carassius carassius)in Taihu Lake,China[J].Environmental Toxicology&Chemistry,2010,26(6):1258-1264
[5]宫云飞,兰冬东,李冕,等.大连市近岸海域溢油污染事故风险受体脆弱性评价研究[J].海洋开发与管理,2015,32(10):66-68Gong Y F,Lan D D,Li M,et al.Vulnerability assessment of risk receptor for oil spill pollution accidents in coastal waters of Dalian[J].Ocean Development and Management,2015,32(10):66-68(in Chinese)
[6]周政权,李晓静,陈琳琳,等.蓬莱19-3平台溢油事故对渤海大型底栖生物群落结构的长期影响[J].广西科学院学报,2016,32(2):92-100Zhou Z Q,Li X J,Chen L L,et al.Long-term effects of Penglai 19-3 Oil Spill Event on the macrobenthic community structure in Bohai Sea[J].Journal of Guangxi Academy of Sciences,2016,32(2):92-100(in Chinese)
[7]高颖,高学文.海水和海洋沉积物中多环芳烃分布的研究进展[J].中国水运,2018,18(8):100-104Gao Y,Gao X W.Research progress on distribution of polycyclic aromatic hydrocarbons in seawater and marine sediments[J].China Water Transport,2018,18(8):100-104(in Chinese)
[8]叶志波,杨乐,邓瑞杰,等.消油剂对海洋溢油中多环芳烃环境行为的影响[J].青岛理工大学学报,2017,38(6):89-94Ye Z B,Yang L,Deng R J,et al.Influence of dispersants on the environmental behavior of polycyclic aromatic hydrocarbons derived from marine spilled oil[J].Journal of Qingdao University of Technology,2017,38(6):89-94(in Chinese)
[9]郑关超,郭萌萌,赵春霞,等.环渤海地区养殖水产品中多环芳烃(PAHs)污染残留及健康风险评估[J].中国渔业质量与标准,2015,5(6):20-26Zheng G C,Guo M M,Zhao C X,et al.Residue levels of PAHs in aquaculture products from Bohai Bay and their health risk assessment[J].Chinese Fishery Quality and Standards,2015,5(6):20-26(in Chinese)
[10]靳非,穆景利,王冬婉,等.菲、3-甲基菲和菲醌对河鲀(Takifugu rubripes)幼鱼肝组织损伤的比较研究[J].生态毒理学报,2016,11(1):248-253Jin F,Mu J L,Wang D W,et al.The comparative research of phenanthrene,3-methyl phenanthrene and phenanthraquinone on the liver tissue injury of pufferfish(Takifugu rubripes)larvae[J].Asian Journal of Ecotoxicology,2016,11(1):248-253(in Chinese)
[11]王晓燕,徐俊,陈宁,等.2,6-及2,7-二甲基蒽的合成与表征[J].青岛科技大学学报:自然科学版,2008,29(1):1-4Wang X Y,Xu J,Chen N,et al.Synthesis and characterization of 2,6-and 2,7-dimethylanthracene[J].Journal of Qingdao University of Science and Technology:Natural Science Edition,2008,29(1):1-4(in Chinese)
[12]王冬婉.菲、3-甲基菲和菲醌对暗纹东方鲀(Takifugu fasciatus)毒性的比较研究[D].大连:大连海事大学,2016:11-16
[13]Latuihamallo M,Iriana D,Apituley D.Amino acid and fatty acid of abalone Haliotis squamata,cultured in different aquaculture systems[J].Procedia Food Science,2015,3:174-181
[14]郭远明,张小军,严忠雍,等.皱纹盘鲍和杂色鲍肌肉主要营养成分的比较[J].营养学报,2014,36(4):403-405Guo Y M,Zhang X J,Yan Z Y,et al.Comparison of nutritional components in muscle of Haliotis discus hanai and Haliotis diversicolor reeve[J].Acta Nutrimenta Simica,2014,36(4):403-405(in Chinese)
[15]李天云,黄圣彪,孙凡,等.河蚬对太湖梅梁湾沉积物多环芳烃的生物富集[J].环境科学学报,2008,28(11):2354-2360Li T Y,Huang S B,Sun F,et al.Bioaccumulation by Corbicula fluminea of polycyclic aromatic hydrocarbons from sediments in Meiliang Bay,Taihu Lake[J].Acta Scientiae Circumstantiae,2008,28(11):2354-2360(in Chinese)
[16]修蒙.多环芳烃(PAHs)在栉孔扇贝体内蓄积特征与毒性效应的研究[D].青岛:中国海洋大学,2014:16-19Xiu M.Study of bioaccumulation and toxicity effects of scallop Chlamys farreri exposed to polycyclic aromatic hydrocarbons(PAHs)[D].Qingdao:Ocean University of China,2014:16-19(in Chinese)
[17]蔡立哲,马丽,袁东星,等.九龙江口红树林区底栖动物体内的多环芳烃[J].海洋学报(中文版),2005(5):112-118Cai L Z,Ma L,Yuan D X,et al.Polycyclic aromatic hydrocarbons in zoobenthos in mangrove swamp in the Jiulong River Estuary[J].Acta Oceanologcica Sinica,2005(5):112-118(in Chinese)
[18]许高鹏,蒋玫,李磊,等.三疣梭子蟹体内苯并[a]芘的富集动力学[J].海洋渔业,2014,36(4):357-363Xu G P,Jiang M,Li L,et al.Kinetics of benzo[a]pyrene bioconcentration in Portunus tritubeiculatus[J].Marine Fisheries,2014,36(4):357-363(in Chinese)
[19]蒋闰兰,肖佰财,禹娜,等.多环芳烃对水生动物毒性效应的研究进展[J].海洋渔业,2014,36(4):372-384Jiang R L,Xiao B C,Yu N,et al.Research advance in toxic effects of PAHs on aquatic animals[J].Marine Fisheries,2014,36(4):372-384(in Chinese)
[20]李天云.利用河蚬研究沉积物中有机氯农药和多环芳烃在生物体内的累积效应[D].重庆:西南大学,2008:17-26Li T Y.Bioaccumulation of organochlorine pesticides(OCPs)and polycyclic aromatic hydrocarbons(PAHs)for Corbicula fluminea from sediment in sewer channel of Tianjin[D].Chongqing:Southwest University,2008:17-26(in Chinese)
[21]李磊,沈新强,李超,等.苯并[a]芘、菲在缢蛏体内的生物富集与释放[J].水产学报,2015,39(7):998-1004Li L,Shen X Q,Li C,et al.Bioaccumulation-depuration kinetics of benzo[a]pyrene and phenanthrene in Sinonovacula constricta[J].Journal of Fisheries of China,2015,39(7):998-1004(in Chinese)
[22]陈辉辉,覃剑晖,刘海超,等.典型重金属、多环芳烃及菊酯类农药对唐鱼的急性毒性效应[J].华中农业大学学报,2011,30(4):511-515Chen H H,Tan J H,Liu H C,et al.Acute toxicity of representative heavy metals,polycyclic aromatic hydrocarbons(PAHs)and pyrethroid pesticide to Tanichthys albonubes[J].Journal of Huazhong Agricultural University,2011,30(4):511-515(in Chinese)
[23]高萍,夏斌,崔毅,等.菲律宾蛤仔对石油烃的富集与释放特征[J].海洋环境科学,2013,32(1):63-66Gao P,Xia B,Cui Y,et al.Accumulation and elimination characteristic of Ruditapes philippinarum to petroleum hydrocarbon[J].Marine Environmental Science,2013,32(1):63-66(in Chinese)
[24]Baussant T,Sanni S,Jonsson G,et al.Bioaccumulation of polycyclic aromatic compounds:1.Bioconcentration in two marine species and in semipermeable membrane devices during chronic exposure to dispersed crude oil[J].Environmental Toxicology&Chemistry,2001,20(6):1175-1184
[25]Dos Santos Fogaca F H,Soares C,Oliveira M,et al.Polycyclic aromatic hydrocarbons bioaccessibility in seafood:Culinary practices effects on dietary exposure[J].Environmental Research,2018,164:165-172
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