荧光观察方法检测水中大肠埃希氏菌的应用
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摘要
目的比对2种荧光观察方法检测生活饮用水中大肠埃希氏菌的适用性、灵敏度及稳定性。方法使用全自动菌落计数器与传统多管发酵法2种方法对大肠埃希氏菌EC-MUG肉汤管进行荧光观察测试、分析。结果仪器法较传统法灵敏,EC-MUG肉汤管培养4h后使用仪器即可观察到蓝色荧光,6h后呈现深蓝色荧光,而传统法在6 h时荧光现象微弱呈淡蓝色, 10 h后荧光现象逐渐明显呈现蓝色。分别使用传统法与仪器法观察10组水样结果无差异。结论观察水中大肠埃希氏菌荧光现象时可使用仪器法和传统法,但仪器法更有优势,灵敏度高,操作简单,值得推广。
Objective To compare the applicability, sensitivity and stability of the 2 kinds of fluorescence observation methods for the detection of Escherichia coli in drinking water. Methods Automatic instrumental method and traditional multi-tube fermentation method were used to observe and analyze the fluorescence of Escherichia coli EC-MUG broth tube. Results The instrumental method was more sensitive than traditional method. The instrumental method showed that blue fluorescence could be observed after 4 h, while it was more obvious after 6 h. Traditional method showed that blue fluorescence gradually became light blue after 6 h, while it was gradually obvious after 10 h. There was no difference between traditional method and instrumental method in stability test. Conclusion Instrumental method and traditional method can be used to observe the fluorescence of Escherichia coli in water, but the instrumental method has more advantages, higher sensitivity and simpler operation, which is worth popularizing.
Objective To compare the applicability, sensitivity and stability of the 2 kinds of fluorescence observation methods for the detection of Escherichia coli in drinking water. Methods Automatic instrumental method and traditional multi-tube fermentation method were used to observe and analyze the fluorescence of Escherichia coli EC-MUG broth tube. Results The instrumental method was more sensitive than traditional method. The instrumental method showed that blue fluorescence could be observed after 4 h, while it was more obvious after 6 h. Traditional method showed that blue fluorescence gradually became light blue after 6 h, while it was gradually obvious after 10 h. There was no difference between traditional method and instrumental method in stability test. Conclusion Instrumental method and traditional method can be used to observe the fluorescence of Escherichia coli in water, but the instrumental method has more advantages, higher sensitivity and simpler operation, which is worth popularizing.
引文
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[11]钟玮,冯震,鲍英,等.金黄色葡萄球菌的API–STAPH生化鉴定及核糖体分型分析[J].中国抗生素杂志,2013,38(5):354-358.Zhong W,Feng Z,Bao Y,et al. Identififi cation of Staphylococcus aureus by API–STAPH test and ribosomal typing analysis[J]. Chin J Antibiot,2013,38(5):354-358.
[12] G6全自动菌落计数分析仪[J].食品安全导刊,2010,(7):33.G6 automatic colony count analyzer[J]. China Food Saf Magaz,2010,(7):33.
[13]许琰,孙双福,崔琳.水中大肠埃希菌多管发酵法测定方法探讨[J].上海预防医学,2013,25(6):340-342.Xu Y,Sun SF,Cui L. Study on the determination method of Escherichia coli in water by multi–tube fermentation[J]. Shanghai J Prev Med,2013,25(6):340-342.
[14]苏德模,徐维洁,刘鹏. MUG快速检定药品中大肠杆菌的方法研究[J].药物分析杂志,1994,14(5):37-39.Su DM,Xu WJ,Liu P. MUG study on the method of rapid identification of Escherichia coli in drugs[J]. Chin J Pharm Anal,1994,14(5):37-39.
[15]袁月明,俞慕华,袁梦.水中大肠菌群和大肠埃希氏菌检测方法的比较[J].河南预防医学杂志,2009,20(3):185-186,216.Yuan YM,Yu MH,Yuan M. Comparison of detection methods of coliforms and Escherichia coli in water[J]. Henan J Prev Med,2009,20(3):185-186,216.
[2]丁程成.欧美水质大肠菌群相关检验标准探究[J].环境科技,2010,23(1):67-69.Ding CC. Research on western relevant inspection standards of coliform group in water body[J]. Environ Sci Technol,2010,23(1):67-69.
[3]张倩华.农村生活饮用水水质检测结果分析[J].当代医学,2012,18(12):161-162.Zhang QH. Analysis of rural drinking water quality test results[J].Contemp Med,2012,18(12):161-162.
[4]邓婷,梁漪莲,骆琼,等.两种方法对水中总大肠菌群和大肠埃希氏菌检测的对比分析[J].食品工程,2017,(1):52-54.Deng T,Liang YL,Luo Q,et al. Two methods'comparative analysis of detecting total coliform bacteria and E. coli bacteria in water[J]. Food Eng,2017,(1):52-54.
[5] Michael PD,Marilyn CE. The fecal coliform assay,the results of which have led to numerous misinterpretations over the years,may have outlived its usefulness[J]. Am Soc Microbiol,2006,32:123-126.
[6] Garcia AT,Prats J,Servais P. Comparison of culturable fecal coliform and E. coli enumeration in freshwaters[J]. Can J Microbiol,2007,53:798-801.
[7] GB 5749-2006生活饮用水卫生标准[S].GB 5749-2006 Sanitary standard for drinking water[S].
[8] GB/T 5750.12-2006生活饮用水标准检验方法微生物指标[S].GB/T 5750.12-2006 Standard test methods for drinking waterMicrobiological indicators[S].
[9]张小东,鄢庆枇,邹文政. MUG单管定量检测法对大肠埃希氏菌的检测[J].集美大学学报,2009,14(4):1-6.Zhang XD,Yan QP,Zou WZ. Detection of Escherichia coli by MUG single tube quantitative detection method[J]. J Jimei Univ,2009,14(4):1-6.
[10]王平,陶红,曹武. API20E鉴定系统在肠杆菌科细菌检验中的应用[J].微生物学杂志,1999,19(3):62.Wang P,Tao H,Cao W. API20E application of identification system in enterobacteriaceae bacteria test[J]. J Microbiol,1999,19(3):62.
[11]钟玮,冯震,鲍英,等.金黄色葡萄球菌的API–STAPH生化鉴定及核糖体分型分析[J].中国抗生素杂志,2013,38(5):354-358.Zhong W,Feng Z,Bao Y,et al. Identififi cation of Staphylococcus aureus by API–STAPH test and ribosomal typing analysis[J]. Chin J Antibiot,2013,38(5):354-358.
[12] G6全自动菌落计数分析仪[J].食品安全导刊,2010,(7):33.G6 automatic colony count analyzer[J]. China Food Saf Magaz,2010,(7):33.
[13]许琰,孙双福,崔琳.水中大肠埃希菌多管发酵法测定方法探讨[J].上海预防医学,2013,25(6):340-342.Xu Y,Sun SF,Cui L. Study on the determination method of Escherichia coli in water by multi–tube fermentation[J]. Shanghai J Prev Med,2013,25(6):340-342.
[14]苏德模,徐维洁,刘鹏. MUG快速检定药品中大肠杆菌的方法研究[J].药物分析杂志,1994,14(5):37-39.Su DM,Xu WJ,Liu P. MUG study on the method of rapid identification of Escherichia coli in drugs[J]. Chin J Pharm Anal,1994,14(5):37-39.
[15]袁月明,俞慕华,袁梦.水中大肠菌群和大肠埃希氏菌检测方法的比较[J].河南预防医学杂志,2009,20(3):185-186,216.Yuan YM,Yu MH,Yuan M. Comparison of detection methods of coliforms and Escherichia coli in water[J]. Henan J Prev Med,2009,20(3):185-186,216.
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