东北地区鸡源多重耐药大肠杆菌整合子的检测

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英文篇名：Detection of Integrons in Multi-drug Resistant Isolates of Escherichia coli from Chicken in Northeastern China 作者：冯涛 ; 何纪元 ; 薛原 英文作者：FENG Tao;HE Ji-yuan;XUE Yuan;College of Wildlife Resources,Northeast Forestiry University; 关键词：鸡 ; 大肠杆菌 ; 耐药性 ; PCR ; 整合子 ; 基因盒 英文关键词：Chicken;;Escherichia coli;;Drug resistance;;PCR;;Integron;;Gene casetes 中文刊名：安徽农业科学 英文刊名：Journal of Anhui Agricultural Sciences 机构：东北林业大学野生动物资源学院; 出版日期：2019-01-18 出版单位：安徽农业科学 年：2019 期：02 基金：国家自然科学基金项目(31502119);; 黑龙江省博士后科研启动项目(LBH-Q14002) 语种：中文; 页：91-93+106 页数：4 CN：34-1076/S ISSN：0517-6611 分类号：S852.61

摘要

[目的]了解东北地区鸡源大肠杆菌耐药性的流行特征以及整合子-基因盒系统的耐药机制。[方法]在检测耐药性的基础上,采用PCR方法对整合酶和整合子进行检测和克隆测序,并且对检测结果和耐药基因盒与GenBank数据库的序列进行比对和分析。[结果]413株分离菌中整合子检出率为71.91%,整合酶Ⅰ和整合酶Ⅱ的检出率分别为65.52%和54.48%,整合酶Ⅲ未检出。序列分析得出,共包含6种整合子,各自携带不同组合的基因盒。[结论]Ⅰ型整合子在细菌耐药性的传播过程中发挥着至关重要的作用。
        [Objective] To investigate the prevalence characteristics of drug-resistant Escherichia coli isolates in Northeastern China and the drug-resistant mechanism of integron-gene cassettes system.[Method]On the basis of drug-resistant detection, integrase and integron were detected,cloned and sequenced by PCR method.The detection results and drug-resistant gene cassettes were blasted with sequences with GenBank database.[Result]The detection rate of integron among 413 strains of isolates was 71.91%.The detection rates of integrase Ⅰ and integrase Ⅱ were 65.52% and 54.48% respectively.Integrase Ⅲ was not detected.There were six types of integrons by sequencing analysis,which carried different combinations of gene cassettes.[Conclusion] Integron Ⅰ played an important role in the propagation process of bacterial resistance.

引文

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