2个种源栓皮栎对干旱及复水的光合生理响应
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摘要
为了探究干旱复水对2个种源栓皮栎幼苗光合生理特性的影响,测定了不同水分处理(正常供水、轻度干旱、中度干旱和重度干旱)及复水处理下盆栽栓皮栎幼苗生长、叶片气体交换及叶绿素荧光参数。结果表明:随着干旱胁迫程度的加重,幼苗生长受到的抑制程度增加;气体交换参数净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和水分利用效率(WUE)均呈下降的趋势,而气孔限制值(Ls)呈上升趋势,在重度干旱胁迫下气体交换参数变化幅度最大;叶片非光化学猝灭系数(NPQ)呈现先上升后下降的趋势,PSII潜在活性(Fv/Fo)、最大光化学效率(Fv/Fm)、电子传递速率(ETR)和实际光量子效率(ΦPSⅡ)均呈下降趋势。主要叶绿素荧光参数对干旱胁迫的响应与气体交换参数一致,即重度胁迫对光合电子传递造成了最严重的损伤。干旱后复水,导致栓皮栎光合生理参数不同程度的恢复。就种源地而言,老君山种源的幼苗在干旱胁迫下主要参数的变化程度大于北坡山种源的幼苗,且在复水后老君山种源幼苗恢复程度低于北坡山种源,表明北坡山种源的栓皮栎抗旱力和恢复力均强于老君山种源。
To investigate the effects of drought stress and subsequent re-watering on photosynthetic characteristics of seedlings from two different provenances of Quercus variabilis Bl.,we measured the growth,gas exchange,and chlorophyll fluorescence parameters of seedlings under different water treatments( normal irrigation,slight drought,moderate drought,and severe drought)and following re-watering. The results showed that the growth of seedlings was inhibited by drought stress,and it was most strongly affected under severe drought stress. Gas exchange parameters,such as Pn,Gs,Trand WUE,had decreasing trends,while Lsshowed the upward trend with the increases of drought stress. Chlorophyll fluorescence parameters,such as Fv/Fo,Fv/Fm,ETR and ΦPSⅡ,showed decreasing trends with the increasing intensity of drought stress.The NPQ value increased under moderate drought and declined under severe drought. The response of many chlorophyll fluorescence parameters to drought stress was consistent with that of some gas exchange parameters. For example,the highest response was found under severe drought. Re-watering after drought stress resulted in somewhat recovery of these physiological parameters. Generally,the relative changes of these parameters of seedlings from the Laojun Mountain provenance were higher than those from the Beipo Mountain provenance,and the recovery of seedlings from Laojun Mountain provenance was lower than that of the Beipo Mountain provenance after re-watering. In conclusion,the drought tolerance and resilience of seedlings from the Beipo Mountain provenance were better than those from Laojun Mountain provenance.
To investigate the effects of drought stress and subsequent re-watering on photosynthetic characteristics of seedlings from two different provenances of Quercus variabilis Bl.,we measured the growth,gas exchange,and chlorophyll fluorescence parameters of seedlings under different water treatments( normal irrigation,slight drought,moderate drought,and severe drought)and following re-watering. The results showed that the growth of seedlings was inhibited by drought stress,and it was most strongly affected under severe drought stress. Gas exchange parameters,such as Pn,Gs,Trand WUE,had decreasing trends,while Lsshowed the upward trend with the increases of drought stress. Chlorophyll fluorescence parameters,such as Fv/Fo,Fv/Fm,ETR and ΦPSⅡ,showed decreasing trends with the increasing intensity of drought stress.The NPQ value increased under moderate drought and declined under severe drought. The response of many chlorophyll fluorescence parameters to drought stress was consistent with that of some gas exchange parameters. For example,the highest response was found under severe drought. Re-watering after drought stress resulted in somewhat recovery of these physiological parameters. Generally,the relative changes of these parameters of seedlings from the Laojun Mountain provenance were higher than those from the Beipo Mountain provenance,and the recovery of seedlings from Laojun Mountain provenance was lower than that of the Beipo Mountain provenance after re-watering. In conclusion,the drought tolerance and resilience of seedlings from the Beipo Mountain provenance were better than those from Laojun Mountain provenance.
引文
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崔豫川,张文辉,李志萍.2014.干旱和复水对栓皮栎幼苗生长和生理特性的影响.林业科学,50(7):66-73.
杜建雄,侯向阳,刘金荣,等.2010.草地早熟禾对干旱及旱后复水的生理响应研究.草业学报,19(2):31-38.
郭子锋,龚道枝,郝卫平,等.2011.不同生育期玉米干旱-复水补偿效应的品种差异研究.玉米科学,19(4):84-88.
郭相平,张烈君,王琴,等.2005.作物水分胁迫补偿效应研究进展.河海大学学报:自然科学版,33(6):634-637.
郭艳阳,刘佳,朱亚利,等.2018.玉米叶片光合和抗氧化酶活性对干旱胁迫的响应.植物生理学报,54(12):1839-1846.
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李娟,彭镇华,高健,等.2011.干旱胁迫下黄条金刚竹的光合和叶绿素荧光特性.应用生态学报,22(6):1395-1402.
李鹏民,高辉远,Strasser RJ.2005.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用.植物生理与分子生物学学报,31(5):559-566.
林祥磊,许振柱,王玉辉,等.2008.羊草(Leymus chinensis)叶片光合参数对干旱与复水的响应机理与模拟.生态学报,28(10):4718-4724.
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刘婷婷,陈道钳,王仕稳,等.2018.不同品种高粱幼苗在干旱复水过程中的生理生态响应.草业学报,27(6):100-110.
罗宏海,张亚黎,张旺锋,等.2008.新疆滴灌棉花花铃期干旱复水对叶片光合特性及产量的影响.作物学报,34(1):171-174.
裴斌.2013.土壤水分胁迫对沙棘叶片光合生理生化特性的影响(博士学位论文).泰安:山东农业大学.
裴斌,张光灿,张淑勇,等.2013.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响.生态学报,33(5):1386-1396.
王满莲,唐辉,韩愈,等.2017.水分胁迫与复水对地枫皮生理生态特性的影响.广西植物,37(6):716-722.
王孝威,段艳红,曹慧,等.2003.水分胁迫对短枝型果树光合作用的非气孔限制.西北植物学报,23(9):1609-1613.
王帆,何奇瑾,周广胜.2019.夏玉米三叶期持续干旱下不同叶位叶片含水量变化及其与光合作用的关系.生态学报,39(1):254-264.
吴敏,张文辉,周建云,等.2014.干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响.生态学报,34(15):4223-4233.
吴玲,李志辉,吴际友,等.2017.干旱胁迫对青冈栎种源叶绿素含量与抗氧化酶活性的影响.中南林业科技大学学报,37(6):51-55.
魏霞,李守中,郑怀周.2007.叶片气体交换和叶绿素荧光在植物逆境生理研究中的应用.福建师范大学学报,23(4):124-128.
向永国,费世民,蒋俊明.1997.柑桔和小麦间作中小麦光合的非气孔限制及其对水分胁迫的反应.四川林业科技,18(2):6-16.
闫志利,轩春香,牛俊义,等.2009.干旱胁迫及复水对豌豆根系内源激素含量的影响.中国生态农业学报,17(2):297-301.
杨晓康.2012.干旱胁迫对不同抗旱花生品种生理特性、产量和品质的影响(博士学位论文).泰安:山东农业大学.
赵雪,张秀珍,牟洪香,等.2017.干旱胁迫对不同种源文冠果幼苗水分生理特性及渗透调节物质的影响.东北林业大学学报,45(6):17-21.
张栋.2011.干旱胁迫对苹果光合作用和叶绿素荧光的影响及叶片衰老特性研究(博士学位论文).杨凌:西北农林科技大学.
张军,王建波,陈刚,等.2009.Na2CO3胁迫下星星草幼苗叶片电解质外渗率与PSⅡ光能耗散的关系.草业学报,18(3):200-206.
郑雨,赵玉文,费文群,等.2018.干旱胁迫下西藏2种牡丹幼苗叶绿素含量和光合特征比较.高原农业,2(5):453-461.
周雪英,邓西平.2007.旱后复水对不同倍性小麦光合及抗氧化特性的影响.西北植物学报,27(2):278-285.
Aidar ST,Meirelles ST,Oliveira RF,et al.2014.Photosynthetic response of poikilochlorophyllous desiccation-tolerant Pleurostima purpurea(Velloziaceae)to dehydration and rehydration.Photosynthetica,52:124-133.
Anjum SA,Xie X,Wang LC,et al.2011.Morphological,physiological and biochemical responses of plants to drought stress.African Journal of Agricultural Research,6:2026-2032.
Calatayud A,Roca D,Martínez PF.2006.Spatial-temporal variations in rose leaves under water stress conditions studied by chlorophyll fluorescence imaging.Plant Physiology and Biochemistry,44:564-573.
Centritto M,Brilli F,Fodale R,et al.2011.Different sensitivity of isoprene emission,respiration and photosynthesis to high growth temperature coupled with drought stress in black poplar(Populus nigra)saplings.Tree Physiology,31:275-286.
Chaves MM,Flexas J,Pinheiro C.2009.Photosynthesis under drought and salt stress:Regulation mechanisms from whole plant to cell.Annals of Botany,103:551-560.
Demirevska K,Zasheva D,Dimitrov R,et al.2009.Drought stress effects on Rubisco in wheat:Changes in the Rubisco large subunit.Acta Physiologiae Plantarum,31:1129-1138.
Farooq M,Hussain M,Wahid A,et al.2012.Drought stress in plants:An overview//Aroca R,ed.Plant Responses to Drought Stress:From Morphological to Molecular Features.Berlin:Springer:1-33.
Fortunati A,Barta C,Brilli F,et al.2008.Isoprene emission is not temperaturedependent during and after severe droughtstress:A physiological and biochemical analysis.Plant Journal,55:687-697.
Galmés J,Medrano H,Flexas J.2007.Photosynthetic limitations in response to water stress and recovery in Mediterranean plants with different growth forms.New Phytologist,175:81-93.
Gessler A,Keitel C,Kreuzwieser J,et al.2007.Potential risks for European beech(Fagus sylvatica L.)in a changing climate.Trees,21:1-11.
Guama J,Linera G.2006.Edge effect on acorn removal and oak seedling survival in Mexican lower montane forest fragments.New Forests,31:487-495.
Khan A,Anwar Y,Hasan MM,et al.2017.Attenuation of drought stress in Brassica seedlings with exogenous application of Ca2+and H2O2.Plants,6:1-13.
Manivannan P,Jaleel CA,Sankar B,et al.2007.Growth,biochemical modifications and proline metabolism in Helianthus annuus L.as induced by drought stress.Colloids and Surfaces B:Biointerfaces,59:141-149.
Matthias Arend L,Thomas K,Madeleine S,et al.2011.Provenance-specific growth responses to drought and air warming in three European oak species(Quercus robur,Q.petraea and Q.pubescens).Tree Physiology,31:287-297.
Maxwell K,Johnson GN.2000.Chlorophyll fluorescence:Apractical guide.Journal of Experimental Botany,51:659-668.
McDowell N,Pockman WT,Allen CD,et al.2008.Mechanisms of plant survival and mortality during drought:Why do some plants survive while others succumb to drought.New Phytologist,178:719-739.
Ohlemüller R,Gritti ES,et al.2006.Quantifying components of risk for European woody species under climate change.Global Change Biology,12:1788-1799.
Samoilova OP,Ptushenko VV,Kuvykin IV,et al.2011.Effects of light environment on the induction of chlorophyll fluorescence in leaves:A comparative study of Tradescantia species of different ecotypes.Bio Systems,105:41-48.
Wang L,Zhang T,Ding SY.2006.Effect of drought and rewatering on photosynthetic physioecological characteristics of soybean.Acta Ecologica Sinica,26:2073-2078.
Zhang J,Kirkham MB.1996.Antioxidant responses to drought in sunflower and sorghum seedlings.New Phytologist,132:361-373.
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崔豫川,张文辉,王校锋.2013.栓皮栎幼苗对土壤干旱胁迫的生理响应.西北植物学报,33(2):364-370.
崔豫川,张文辉,李志萍.2014.干旱和复水对栓皮栎幼苗生长和生理特性的影响.林业科学,50(7):66-73.
杜建雄,侯向阳,刘金荣,等.2010.草地早熟禾对干旱及旱后复水的生理响应研究.草业学报,19(2):31-38.
郭子锋,龚道枝,郝卫平,等.2011.不同生育期玉米干旱-复水补偿效应的品种差异研究.玉米科学,19(4):84-88.
郭相平,张烈君,王琴,等.2005.作物水分胁迫补偿效应研究进展.河海大学学报:自然科学版,33(6):634-637.
郭艳阳,刘佳,朱亚利,等.2018.玉米叶片光合和抗氧化酶活性对干旱胁迫的响应.植物生理学报,54(12):1839-1846.
井大炜,邢尚军,马海林,等.2014.I-107欧美杨对不同强度干旱胁迫的形态与生理响应.东北林业大学学报,42(1):10-13.
李娟,彭镇华,高健,等.2011.干旱胁迫下黄条金刚竹的光合和叶绿素荧光特性.应用生态学报,22(6):1395-1402.
李鹏民,高辉远,Strasser RJ.2005.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用.植物生理与分子生物学学报,31(5):559-566.
林祥磊,许振柱,王玉辉,等.2008.羊草(Leymus chinensis)叶片光合参数对干旱与复水的响应机理与模拟.生态学报,28(10):4718-4724.
刘菲,周隆腾,蒋燚,等.2018.不同种源江南油杉幼苗对干旱胁迫的生理响应.中南林业科技大学学报,38(11):35-45.
刘婷婷,陈道钳,王仕稳,等.2018.不同品种高粱幼苗在干旱复水过程中的生理生态响应.草业学报,27(6):100-110.
罗宏海,张亚黎,张旺锋,等.2008.新疆滴灌棉花花铃期干旱复水对叶片光合特性及产量的影响.作物学报,34(1):171-174.
裴斌.2013.土壤水分胁迫对沙棘叶片光合生理生化特性的影响(博士学位论文).泰安:山东农业大学.
裴斌,张光灿,张淑勇,等.2013.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响.生态学报,33(5):1386-1396.
王满莲,唐辉,韩愈,等.2017.水分胁迫与复水对地枫皮生理生态特性的影响.广西植物,37(6):716-722.
王孝威,段艳红,曹慧,等.2003.水分胁迫对短枝型果树光合作用的非气孔限制.西北植物学报,23(9):1609-1613.
王帆,何奇瑾,周广胜.2019.夏玉米三叶期持续干旱下不同叶位叶片含水量变化及其与光合作用的关系.生态学报,39(1):254-264.
吴敏,张文辉,周建云,等.2014.干旱胁迫对栓皮栎幼苗细根的生长与生理生化指标的影响.生态学报,34(15):4223-4233.
吴玲,李志辉,吴际友,等.2017.干旱胁迫对青冈栎种源叶绿素含量与抗氧化酶活性的影响.中南林业科技大学学报,37(6):51-55.
魏霞,李守中,郑怀周.2007.叶片气体交换和叶绿素荧光在植物逆境生理研究中的应用.福建师范大学学报,23(4):124-128.
向永国,费世民,蒋俊明.1997.柑桔和小麦间作中小麦光合的非气孔限制及其对水分胁迫的反应.四川林业科技,18(2):6-16.
闫志利,轩春香,牛俊义,等.2009.干旱胁迫及复水对豌豆根系内源激素含量的影响.中国生态农业学报,17(2):297-301.
杨晓康.2012.干旱胁迫对不同抗旱花生品种生理特性、产量和品质的影响(博士学位论文).泰安:山东农业大学.
赵雪,张秀珍,牟洪香,等.2017.干旱胁迫对不同种源文冠果幼苗水分生理特性及渗透调节物质的影响.东北林业大学学报,45(6):17-21.
张栋.2011.干旱胁迫对苹果光合作用和叶绿素荧光的影响及叶片衰老特性研究(博士学位论文).杨凌:西北农林科技大学.
张军,王建波,陈刚,等.2009.Na2CO3胁迫下星星草幼苗叶片电解质外渗率与PSⅡ光能耗散的关系.草业学报,18(3):200-206.
郑雨,赵玉文,费文群,等.2018.干旱胁迫下西藏2种牡丹幼苗叶绿素含量和光合特征比较.高原农业,2(5):453-461.
周雪英,邓西平.2007.旱后复水对不同倍性小麦光合及抗氧化特性的影响.西北植物学报,27(2):278-285.
Aidar ST,Meirelles ST,Oliveira RF,et al.2014.Photosynthetic response of poikilochlorophyllous desiccation-tolerant Pleurostima purpurea(Velloziaceae)to dehydration and rehydration.Photosynthetica,52:124-133.
Anjum SA,Xie X,Wang LC,et al.2011.Morphological,physiological and biochemical responses of plants to drought stress.African Journal of Agricultural Research,6:2026-2032.
Calatayud A,Roca D,Martínez PF.2006.Spatial-temporal variations in rose leaves under water stress conditions studied by chlorophyll fluorescence imaging.Plant Physiology and Biochemistry,44:564-573.
Centritto M,Brilli F,Fodale R,et al.2011.Different sensitivity of isoprene emission,respiration and photosynthesis to high growth temperature coupled with drought stress in black poplar(Populus nigra)saplings.Tree Physiology,31:275-286.
Chaves MM,Flexas J,Pinheiro C.2009.Photosynthesis under drought and salt stress:Regulation mechanisms from whole plant to cell.Annals of Botany,103:551-560.
Demirevska K,Zasheva D,Dimitrov R,et al.2009.Drought stress effects on Rubisco in wheat:Changes in the Rubisco large subunit.Acta Physiologiae Plantarum,31:1129-1138.
Farooq M,Hussain M,Wahid A,et al.2012.Drought stress in plants:An overview//Aroca R,ed.Plant Responses to Drought Stress:From Morphological to Molecular Features.Berlin:Springer:1-33.
Fortunati A,Barta C,Brilli F,et al.2008.Isoprene emission is not temperaturedependent during and after severe droughtstress:A physiological and biochemical analysis.Plant Journal,55:687-697.
Galmés J,Medrano H,Flexas J.2007.Photosynthetic limitations in response to water stress and recovery in Mediterranean plants with different growth forms.New Phytologist,175:81-93.
Gessler A,Keitel C,Kreuzwieser J,et al.2007.Potential risks for European beech(Fagus sylvatica L.)in a changing climate.Trees,21:1-11.
Guama J,Linera G.2006.Edge effect on acorn removal and oak seedling survival in Mexican lower montane forest fragments.New Forests,31:487-495.
Khan A,Anwar Y,Hasan MM,et al.2017.Attenuation of drought stress in Brassica seedlings with exogenous application of Ca2+and H2O2.Plants,6:1-13.
Manivannan P,Jaleel CA,Sankar B,et al.2007.Growth,biochemical modifications and proline metabolism in Helianthus annuus L.as induced by drought stress.Colloids and Surfaces B:Biointerfaces,59:141-149.
Matthias Arend L,Thomas K,Madeleine S,et al.2011.Provenance-specific growth responses to drought and air warming in three European oak species(Quercus robur,Q.petraea and Q.pubescens).Tree Physiology,31:287-297.
Maxwell K,Johnson GN.2000.Chlorophyll fluorescence:Apractical guide.Journal of Experimental Botany,51:659-668.
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