桦褐孔菌多糖的优化提取及对小鼠高糖高脂诱导的非酒精性脂肪肝的影响
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摘要
通过正交试验优化桦褐孔菌(Phaeoporus obliquus J. Schroet)多糖的提取,并作用于高糖高脂诱导的非酒精性脂肪肝小鼠,来探讨桦褐孔菌多糖对小鼠高糖高脂诱导的非酒精性脂肪肝病的作用效果。通过正交试验优化桦褐孔菌多糖的提取,将多糖作用于高糖高脂诱导的非酒精性脂肪肝小鼠,用ELISA方法检测小鼠血清中的相关指标。结果表明,与正常组比较,模型组小鼠肝脏明显增大,肝指数明显升高(P<0.01),与模型组比较,优化提取的不同浓度桦褐孔菌多糖提取物使小鼠肝脏指数明显下降,对肝脏有不同程度的改善,空腹血糖值下降明显(P<0.01),高浓度给药组(120 mg/kg)使小鼠血清中TC、LDL-C、HDL-C及ALT水平下降较明显(P<0.01),但TG水平变化不大。病理切片结果显示,桦褐孔菌多糖提取物给药组小鼠肝细胞破坏程度有明显改善,高浓度给药组小鼠肝细胞排列更紧密,中央静脉受累情况有明显改善,说明桦褐孔菌多糖提取物对高糖高脂诱导的小鼠非酒精性脂肪肝有一定的治疗效果,其机制可能是与降低血脂有关。
To study the effects of Phaeoporus obliquus polysaccharose to non-alcoholic fatty liver disease in model mice induced by high-fat-high-fructose diet.Optimizing the extraction of Phaeoporus obliquus polysaccharose by orthogonal experiments,and the polysaccharide was used in mice with high sugar and fat-induced non-alcoholic fatty liver.Results showed that compared with normal group,model group mice liver increased obviously,and liver index increased significantly(P<0.01).Compared with the model group,the mice liver index significantly decreased by the extraction of polysaccharides of Phaeoporus obliquus.The livers had different degrees of improvement,and the fasting blood sugar levels decreased significantly(P<0.01).The mice serum TC,LDL-C,HDL-C and ALT levels decreased significantly the in high dosage group(120 mg/kg)(P <0.01),except TG.Pathological results showed that mice liver cell damage have significantly improved with Phaeoporus obliquus polysaccharose.The liver cells arranged closer,central vein situation has improved significantly in high dose group.Phaeoporus obliquus polysaccharose has therapeutic effect in non-alcoholic fatty liver disease in model mice,and its mechanism may be related to lower blood lipids.
To study the effects of Phaeoporus obliquus polysaccharose to non-alcoholic fatty liver disease in model mice induced by high-fat-high-fructose diet.Optimizing the extraction of Phaeoporus obliquus polysaccharose by orthogonal experiments,and the polysaccharide was used in mice with high sugar and fat-induced non-alcoholic fatty liver.Results showed that compared with normal group,model group mice liver increased obviously,and liver index increased significantly(P<0.01).Compared with the model group,the mice liver index significantly decreased by the extraction of polysaccharides of Phaeoporus obliquus.The livers had different degrees of improvement,and the fasting blood sugar levels decreased significantly(P<0.01).The mice serum TC,LDL-C,HDL-C and ALT levels decreased significantly the in high dosage group(120 mg/kg)(P <0.01),except TG.Pathological results showed that mice liver cell damage have significantly improved with Phaeoporus obliquus polysaccharose.The liver cells arranged closer,central vein situation has improved significantly in high dose group.Phaeoporus obliquus polysaccharose has therapeutic effect in non-alcoholic fatty liver disease in model mice,and its mechanism may be related to lower blood lipids.
引文
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[7]严煜钧,刘仲华,林勇,等.茶叶中EGCG对绯鞠婧下脂肪肝大鼠的调脂保肝作用研究[J].茶叶科学,2014,34(3):221-229.
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[9]曹昌权,刘维洲.125例青年缺血性脑卒中的病因及危险因素分析[J].卒中与神经疾病,2014,21(4):34-237.
[10]王昱.油橄榄叶提取物对非酒精性脂肪肝小鼠TLR4-p38MAPK信号通路的干预作用[J].北京联合大学学报,2018,32(3):59-63.
[11]周琴,段丽,吴利春,等.竹节参与丹参和山楂复方改善小鼠非酒精性脂肪肝作用的实验研究[J].中国临床药理学杂志,2018,34(13):1532-1535.
[12]张丽静.芍药苷对小鼠高脂诱导的非酒精性脂肪肝的保护作用及机制研究[D].武汉:武汉大学,2016.
[2] CUI Y,KIM D S,PARK K C. Antioxidant effect of Inonotus obliquus[J].Journal of ethno-pharmacology,2005,96:79-85.
[3] KIM Y O,PARK H W,KIM J H,et al. Anti-cancer effect and sructural characterization of endo-polysacchride from cultivated mycelia of Inonotus obliquus[J].Life science,2006,79:72-80.
[4] SONG H S,LEE Y J,KIM S K,et al. Down regulatory effect of AGI-1120(α-glucosidase inhibitor)and Chagamushoom(Inonotus obliquus)on cellular NF-kB activation and their antioxidant activity[J].Korean Jphar-macogen,2004,35:92-97.
[5]黄年来.俄罗斯神秘的民间药-桦褐孔菌[J].中国食用菌,2002,21(4):7-8.
[6]金光,杨恩月,洪淳赞.桦褐孔菌多糖抗肿瘤作用的实验研究[J].延边大学医学学报,2004,27(4):257.
[7]严煜钧,刘仲华,林勇,等.茶叶中EGCG对绯鞠婧下脂肪肝大鼠的调脂保肝作用研究[J].茶叶科学,2014,34(3):221-229.
[8]杜秀菊,徐伟,穆红梅,等.桦褐孔菌多糖的药理活性与化学结构研究进展[J].食用菌学报,2012,19(1):100-104.
[9]曹昌权,刘维洲.125例青年缺血性脑卒中的病因及危险因素分析[J].卒中与神经疾病,2014,21(4):34-237.
[10]王昱.油橄榄叶提取物对非酒精性脂肪肝小鼠TLR4-p38MAPK信号通路的干预作用[J].北京联合大学学报,2018,32(3):59-63.
[11]周琴,段丽,吴利春,等.竹节参与丹参和山楂复方改善小鼠非酒精性脂肪肝作用的实验研究[J].中国临床药理学杂志,2018,34(13):1532-1535.
[12]张丽静.芍药苷对小鼠高脂诱导的非酒精性脂肪肝的保护作用及机制研究[D].武汉:武汉大学,2016.
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