氮添加对退化高寒草地土壤微生物量碳氮的影响
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摘要
若尔盖高寒草地生态系统脆弱,对环境因子的改变响应敏感。本试验以若尔盖高寒退化草地为研究对象,在2015-2016年每年返青期,以尿素作为氮源在野外开展控制试验,4个氮处理分别为CK(0 g·m~(-2)·a~(-1))、N_5(5 g·m~(-2)·a~(-1))、N_(10)(10 g·m~(-2)·a~(-1))、N_(20)(20 g·m~(-2)·a~(-1)),分析了氮添加下4个不同退化程度的高寒草地土壤微生物量碳氮以及土壤理化性质的变化规律,探讨若尔盖高寒草地对氮添加的响应机制,旨在为脆弱生境草地的治理与恢复提供参考。结果表明,不同退化草地的土壤微生物量碳氮对氮添加的敏感性随退化程度加剧而逐渐降低。氮浓度20 g·m~(-2)·a~(-1)处理下土壤微生物量碳氮含量变化趋势发生显著变化:轻度退化草地>未退化草地>中度草地>重度退化草地。相关分析表明,土壤微生物量碳氮与速效磷、硝态氮、全氮、全磷、有机碳具有显著正相关,可在一定程度上表征土壤养分状况。氮添加下,土壤微生物量碳氮与土壤理化性质的相关关系发生变化,尤其在N_(20)处理下土壤微生物量碳、氮与其他理化因子间无显著相关关系,需要进一步从土壤微生物对土壤养分的吸收利用方面解释其原因。氮浓度变化显著改变土壤微生物C/N:CK重度退化草地的土壤微生物量碳氮比显著高于其他3个退化样地。N_5和N_(10)条件下不同退化草地土壤微生物C/N无显著差异,而N_(20)处理下未退化草地土壤微生物C/N与CK比显著提高33.7%,而重度退化草地与CK比下降了62.5%,说明氮添加在一定程度上对土壤微生物的组成和群落结构产生了影响。
Alpine meadow is a fragile and vulnerable ecotone in northern Tibet, characterized by high altitude, low temperatures and limited precipitation. Low soil nitrogen availability in these meadows is a key factor limiting the productivity of the grassland. Increasing nitrogen deposition can change C and N cycling dynamics in this ecosystem. The research reported in this paper explored the impact of N addition on soil microbial biomass C and N for four grassland categories in the alpine meadow region of Zoige County: non-degradation grassland(NDG), light degradation grassland(LDG), moderate degradation grassland(MDG), and serious degradation grassland(SDG). In 2015 and 2016, urea was applied to plots during the return of green growth in spring, at rates of nitrogen concentrations 0, 5, 10 and 20 g·m~(-2)(denoted CK, N_5, N_(10) and N_(20), respectively), in which the nitrogen concentrations of 5, 10 and 20 g·m~(-2) were equivalent to urea application amounts of 10.78, 21.55, 43.10 g·m~(-2). The results showed that soil microbial biomass C and N were sensitive to N addition but this response diminished with incrfeasing level of grassland degradation. N addition changed the relationship between soil microbial biomass C, microbial biomass N, and also other soil parameters, such as available phosphorus, nitrate nitrogen, total phosphorus, total nitrogen, total organic carbon, pH, and soil moisture content. Correlation of soil microbial biomass C or N with and other environmental factors was not significant, especially for the N_(20) treatment. Grassland degradation and nitrogen addition can also greatly impact on the ratio of soil microbial biomass C and soil microbial biomass N(SMBC∶SMBN). For the CK treatment, SMBC∶SMBN was significantly higher in serious degradation grassland than in the other three grassland degradation categories. Under the N_5 and N_(10) treatments, SMBC∶SMBN showed no significant difference between plots of the four degradation categories. However, in non-degradation grassland, the SMBC∶SMBN for the N_(20) treatment was 33.7% higher than in the CK treatment. In contrast, in serious degradation grassland, SMBC∶SMBN decreased 62.5% for the N_(20) treatment, compared to the CK treatment. The results indicate that the structure and composition of the soil microbial community is significantly changed following N fertilizer addition to the alpine meadow grassland.
Alpine meadow is a fragile and vulnerable ecotone in northern Tibet, characterized by high altitude, low temperatures and limited precipitation. Low soil nitrogen availability in these meadows is a key factor limiting the productivity of the grassland. Increasing nitrogen deposition can change C and N cycling dynamics in this ecosystem. The research reported in this paper explored the impact of N addition on soil microbial biomass C and N for four grassland categories in the alpine meadow region of Zoige County: non-degradation grassland(NDG), light degradation grassland(LDG), moderate degradation grassland(MDG), and serious degradation grassland(SDG). In 2015 and 2016, urea was applied to plots during the return of green growth in spring, at rates of nitrogen concentrations 0, 5, 10 and 20 g·m~(-2)(denoted CK, N_5, N_(10) and N_(20), respectively), in which the nitrogen concentrations of 5, 10 and 20 g·m~(-2) were equivalent to urea application amounts of 10.78, 21.55, 43.10 g·m~(-2). The results showed that soil microbial biomass C and N were sensitive to N addition but this response diminished with incrfeasing level of grassland degradation. N addition changed the relationship between soil microbial biomass C, microbial biomass N, and also other soil parameters, such as available phosphorus, nitrate nitrogen, total phosphorus, total nitrogen, total organic carbon, pH, and soil moisture content. Correlation of soil microbial biomass C or N with and other environmental factors was not significant, especially for the N_(20) treatment. Grassland degradation and nitrogen addition can also greatly impact on the ratio of soil microbial biomass C and soil microbial biomass N(SMBC∶SMBN). For the CK treatment, SMBC∶SMBN was significantly higher in serious degradation grassland than in the other three grassland degradation categories. Under the N_5 and N_(10) treatments, SMBC∶SMBN showed no significant difference between plots of the four degradation categories. However, in non-degradation grassland, the SMBC∶SMBN for the N_(20) treatment was 33.7% higher than in the CK treatment. In contrast, in serious degradation grassland, SMBC∶SMBN decreased 62.5% for the N_(20) treatment, compared to the CK treatment. The results indicate that the structure and composition of the soil microbial community is significantly changed following N fertilizer addition to the alpine meadow grassland.
引文
[1] Hooper D U,Johnson L.Nitrogen limitation in dryland ecosystems:Responses to geographical and temporal variation in precipitation.Biogeochemistry,1999,46(1/3):247-293.
[2] Liu X,Zhang Y,Han W,et al.Enhanced nitrogen deposition over China.Nature,2013,494:459-462.
[3] Clark C M,Tilman D.Loss of plant species after chronic low-level nitrogen deposition to prairie grasslands.Nature,2008,451:712-715.
[4] Li K,Liu X,Song L,et al.Response of alpine grassland to elevated nitrogen deposition and water supply in China.Oecologia,2015,177(1):65-72.
[5] Zhang F,Huang F Q,Xiao X P,et al.Short-term influences of winter crops on microbial biomass carbon,microbial biomass nitrogen and Cmic-to-Corg in a paddy soil.Acta Ecological Sinica,2009,29(2):734-739.张帆,黄凤球,肖小平,等.冬季作物对稻田土壤微生物量碳、氮和微生物熵的短期影响.生态学报,2009,29(2):734-739.
[6] Sparling G P.Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter.Soil Research,1992,30(2):195-207.
[7] Yu S,Li Y.Study on the microbial biomass as a bioindicator of soil quality in the red earth ecosystem.Acta Pedologica Sinica,1999,36(3):413-422.俞慎,李勇.土壤微生物生物量作为红壤质量生物指标的探讨.土壤学报,1999,36(3):413-422.
[8] Yang N,Zou D S,Yang M Y,et al.Variations of soil microbial biomass carbon and biological information entropy during revegetation on hillslope land with purple soil.Bulletin of Soil and Water Conservation,2014,34(5):39-43.杨宁,邹冬生,杨满元,等.紫色土丘陵坡地植被恢复过程中土壤微生物生物量碳、微生物熵的变化.水土保持通报,2014,34(5):39-43.
[9] Yu P J,Fan G H,Han K X,et al.Soil quality assessment based on soil microbial biomass carbon and soil enzyme activities.Research of Agricultural Modernization,2018,39(1):163-169.禹朴家,范高华,韩可欣,等.基于土壤微生物生物量碳和酶活性指标的土壤肥力质量评价初探.农业现代化研究,2018,39(1):163-169.
[10] Zeng Q C,Li X,Dong Y H,et al.Soil microbial biomass nitrogen and carbon,water soluble nitrogen and carbon under different arbors forests on the Loess Plateau.Acta Ecological Sinica,2015,35(11):3598-3605.曾全超,李鑫,董扬红,等.黄土高原不同乔木林土壤微生物量碳氮和溶解性碳氮的特征.生态学报,2015,35(11):3598-3605.
[11] Zhang C X,Nan Z B.Research progress of soil microbial biomass in China.Pratacultural Science,2010,27(6):50-57.张成霞,南志标.土壤微生物生物量的研究进展.草业科学,2010,27(6):50-57.
[12] Meng J W,Zuo L,Cai Y J,et al.An analysis of desertification status and its preventive strategies in Zoige County.Journal of Sichuan Forestry Science and Technology,2013,34(4):42-46.蒙嘉文,左林,蔡应君,等.若尔盖县土地沙化现状分析及治理对策研究.四川林业科技,2013,34(4):42-46.
[13] Xu G,Zhao Z Z,Wang Y,et al.Remote- sensing dynamic monitoring of sand- and rock-desertification of the Zoigê Plateau basin,northern Sichuan,China.Geological Bulletin of China,2007,26(8):1048-1055.徐刚,赵志中,王燕,等.川北若尔盖高原盆地沙漠化、岩漠化遥感动态监测研究.地质通报,2007,26(8):1048-1055.
[14] Li S J,Jia C.Formation reasons and restoration strategy of the alpine degraded grassland in northwest of Sichuan.Journal of Sichuan Forestry Science and Technology,2013,34(6):89-92.李守剑,贾程.川西北高寒草地退化成因及恢复对策.四川林业科技,2013,34(6):89-92.
[15] Bowman W D,Theodose T A,Schardt J C,et al.Constraints of nutrient availability on primary production in two alpine tundra communities.Ecology,1993,74(7):2085-2097.
[16] Fenn M E,Poth M A,Aber J D,et al.Nitrogen excess in North American ecosystems:Predisposing factors,ecosystem responses,and management strategies.Ecological Applications,1998,8(3):706-733.
[17] Zong N,Shi P L,Jiang J,et al.Interactive effects of short-term nitrogen enrichment and simulated grazing on ecosystem respiration in an alpine meadow on the Tibetan Plateau.Acta Ecologica Sinica,2013,33(19):6191-6201.宗宁,石培礼,蒋婧,等.短期氮素添加和模拟放牧对青藏高原高寒草甸生态系统呼吸的影响.生态学报,2013,33(19):6191-6201.
[18] Dijkstra F A,Hobbie S E,Reich P B,et al.Divergent effects of elevated CO2,N fertilization,and plant diversity on soil C and N dynamics in a grassland field experiment.Plant & Soil,2005,272(1/2):41-52.
[19] Liu W,Xu W,Han Y,et al.Responses of microbial biomass and respiration of soil to topography,burning,and nitrogen fertilization in a temperate steppe.Biology & Fertility of Soils,2007,44(2):259-268.
[20] He Y L,Qi Y C,Peng Q,et al.Effects of exogenous carbon and nitrogen addition on the key process of carbon cycle in grassland ecosystem:A review.China Environmental Science,2018,38(3):1133-1141.贺云龙,齐玉春,彭琴,等.外源碳氮添加对草地碳循环关键过程的影响.中国环境科学,2018,38(3):1133-1141.
[21] Cheng W Y,Qi D C,Li G Y,et al.Effects of fertilization on grass community diversity and productivity of degraded alpine grassland at Maqu,in south of Gansu Province.Journal of China Agricultural University,2009,14(6):31-36.陈文业,戚登臣,李广宇,等.施肥对甘南高寒草甸退化草地植物群落多样性和生产力的影响.中国农业大学学报,2009,14(6):31-36.
[22] Wang G F,An S Z.Effect of fertilizers on soil fertility,population structure and yield of herbages in degraded grassland.Modern Agricultural Science and Technology,2010,(9):278.王高峰,安沙舟.施肥对退化草甸草地土壤肥力和牧草群落结构及产量的影响.现代农业科技,2010,(9):278.
[23] De K J,Zhang D G,Wang W,et al.Effect of the fertilizers on the Alpine Meadow plant and soil N,P,K,contents.Acta Agrestia Sinica,2014,22(2):299-305.德科加,张德罡,王伟,等.施肥对高寒草甸植物及土壤N,P,K的影响.草地学报,2014,22(2):299-305.
[24] Liu X D,Yin G L,Wu J,et al.Effects of nitrogen addition on the physical properties of soil in an al-pine meadow on the eastern Qinghai-Tibetan Plateau.Acta Prataculturae Sinica,2015,24(10):12-21.刘晓东,尹国丽,武均,等.青藏高原东部高寒草甸草地土壤物理性状对氮元素添加的响应.草业学报,2015,24(10):12-21.
[25] Shui W,Bai J P,Jian X M,et al.Changes in water conservation and soil physicochemical properties during the recovery of desertified grassland in Zoigê,China.Acta Ecologica Sinica,2017,37(1):277-285.税伟,白剑平,简小枚,等.若尔盖沙化草地恢复过程中土壤特性及水源涵养功能.生态学报,2017,37(1):277-285.
[26] Su D X,Zhang Z H,Chen Z Z,et al.Parameters for degradation,sandification and salification of rangelands,GB 19377-2003.Beijing:Standards Press of China,2003:1-3.苏大学,张自和,陈佐忠,等.天然草地退化、沙化、盐渍化的分级标准,GB 19377-2003.北京:中国标准出版社,2003:1-3.
[27] Chinese Academy of Sciences,Institute of Soil Science.Soil nutrient analysis//Soil physical and chemical analysis.Shanghai:Shanghai Scientific & Technical Publishers,1978:15-17.中国科学院南京土壤研究所.土壤养分分析//土壤理化分析.上海:上海科学技术出版社,1978:15-17.
[28] Liu G S.Analysis of soil chemical properties and constant nutrients//Soil physical and chemical analysis and description of soil profiles.Beijing:Standard Press of China,1997:33-43.刘光崧.土壤化学性质及常量养分分析//土壤理化分析与剖面描述.北京:中国标准出版社,1997:33-43.
[29] Mo X X,Ye G F,You S S,et al.Soil microbial biomass and their relationships with soil nutrients in different vegetation restoration patterns on degraded coastal sand.Journal of Fujian College of Forestry,2013,33(2):146-150.莫小香,叶功富,游水生,等.海岸退化沙地不同植被恢复模式的土壤微生物量及其与土壤养分的关系.福建林学院学报,2013,33(2):146-150.
[30] Cai X B,Zhou J,Qian C.Variation of soil microbial activities in alpine steppe different in degradation intensity in the north Tibet Platen.Acta Pedologica Sinica,2008,45(6):1110-1118.蔡晓布,周进,钱成.不同退化程度高寒草原土壤微生物活性变化特征研究.土壤学报,2008,45(6):1110-1118.
[31] Bradley K,Drijber R A,Knops J.Increased N availability in grassland soils modifies their microbial communities and decreases the abundance of arbuscular mycorrhizal fungi.Soil Biology & Biochemistry,2006,38(7):1583-1595.
[32] Zhao L.Effects of nitrogen addition on the soil nutrient and microbial biomass in root zone of Medicago sativa.Reasearch of Soil and Water Conservation,2014,21(6):35-40.赵莉.氮添加对紫花苜蓿根区土壤养分及土壤微生物量的影响.水土保持研究,2014,21(6):35-40.
[33] He Y T,Dong Y S,Qi Y C,et al.Advances in researches on soil microbial biomass of grassland ecosystems and its influencing factors.Progress in Geography,2010,29(11):1350-1359.何亚婷,董云社,齐玉春,等.草地生态系统土壤微生物量及其影响因子研究进展.地理科学进展,2010,29(11):1350-1359.
[34] Lin L,Wu Y N,Kenji T,et al.Variation of soil physicochemical and microbial properties in degraded steppes in Hulunbeir of China.Chinese Journal of Applied Ecology,2013,24(12):3407-3414.林璐,乌云娜,田村宪司,等.呼伦贝尔典型退化草原土壤理化与微生物性状.应用生态学报,2013,24(12):3407-3414.
[35] Yang Z A.A study on the responses of vegetation-soil system to grazing and nitrogen addition in an alpine meadow of Qinghai-Tibetan plateau,China.Yangling:Northwest Agricultural and Forestry University,2017.杨振安.青藏高原高寒草甸植被土壤系统对放牧和氮添加的响应研究.杨凌:西北农林科技大学,2017.
[36] Liu J S,Dai J,Liu Y,et al.Effects of excessive nitrogen fertilization on soil organic carbon and nitrogen and nitrogen supply capacity in dryland.Journal of Plant Nutrition and Fertilizer,2015,21(1):112-120.刘金山,戴健,刘洋,等.过量施氮对旱地土壤碳、氮及供氮能力的影响.植物营养与肥料学报,2015,21(1):112-120.
[37] Liu W Q,Liu B Y,Wang J,et al.Response of soil microbial communities to moss cover and nitrogen addition.Acta Ecologica Sinica,2010,30(7):1691-1698.刘蔚秋,刘滨扬,王江,等.不同环境条件下土壤微生物对模拟大气氮沉降的响应.生态学报,2010,30(7):1691-1698.
[38] Wang C T,Wang G X,Liu W,et al.Effects of fertilization gradients on plant community structure and soil characteristics in alpine meadow.Acta Ecologica Sinica,2013,33(10):3103-3113.王长庭,王根绪,刘伟,等.施肥梯度对高寒草甸群落结构、功能和土壤质量的影响.生态学报,2013,33(10):3103-3113.
[39] Wu J S,Lin Q M,Huang Q Y,et al.Soil microbial biomass determination method and its application.Beijing:China Meteorological Press,2006:71-75.吴金水,林启美,黄巧云,等.土壤微生物生物量测定方法及其应用.北京:气象出版社,2006:71-75.
[40] Wang H H,Ren T B,Yuan Y,et al.Combined application of biomass charcoal and nitrogen fertilizer:Effects on microbial biomass C,microbial biomass N and C/N in tobacco soil.Chinese Agricultural Science Bulletin,2017,33(12):52-57.王欢欢,任天宝,元野,等.生物质炭与氮肥配施对植烟土壤微生物量碳、氮和碳氮比的影响.中国农学通报,2017,33(12):52-57.
[41] Li F,Liu Z H,Jia T H,et al.Functional diversity of soil microbial community carbon metabolism with the degradation and restoration of alpine wetlands and meadows.Acta Ecologica Sinica,2018,38(17):6006-6015.李飞,刘振恒,贾甜华,等.高寒湿地和草甸退化及恢复对土壤微生物碳代谢功能多样性的影响.生态学报,2018,38(17):6006-6015.
[42] Zhu R F,Tang F L,Liu J L,et al.Response of soil microbial biomass carbon and nitrogen on a short-term fertilizing N in Leymus chinensis meadow.Acta Agrestia Sinica,2016,24(3):553-558.朱瑞芬,唐凤兰,刘杰淋,等.羊草草甸草原土壤微生物生物量碳氮对短期施氮的响应.草地学报,2016,24(3):553-558.
[43] Dai D,Zi H B,Yang Y F,et al.Responses of soil microbial functional diversity to nitrogen addition in an alpine meadow on Northwestern Plateau of Sichuan Province.Research of Soil and Water Conservation,2018,25(2):137-144.代迪,字洪标,杨有芳,等.高寒草甸土壤微生物功能多样性对氮肥添加的响应.水土保持研究,2018,25(2):137-144.
[2] Liu X,Zhang Y,Han W,et al.Enhanced nitrogen deposition over China.Nature,2013,494:459-462.
[3] Clark C M,Tilman D.Loss of plant species after chronic low-level nitrogen deposition to prairie grasslands.Nature,2008,451:712-715.
[4] Li K,Liu X,Song L,et al.Response of alpine grassland to elevated nitrogen deposition and water supply in China.Oecologia,2015,177(1):65-72.
[5] Zhang F,Huang F Q,Xiao X P,et al.Short-term influences of winter crops on microbial biomass carbon,microbial biomass nitrogen and Cmic-to-Corg in a paddy soil.Acta Ecological Sinica,2009,29(2):734-739.张帆,黄凤球,肖小平,等.冬季作物对稻田土壤微生物量碳、氮和微生物熵的短期影响.生态学报,2009,29(2):734-739.
[6] Sparling G P.Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter.Soil Research,1992,30(2):195-207.
[7] Yu S,Li Y.Study on the microbial biomass as a bioindicator of soil quality in the red earth ecosystem.Acta Pedologica Sinica,1999,36(3):413-422.俞慎,李勇.土壤微生物生物量作为红壤质量生物指标的探讨.土壤学报,1999,36(3):413-422.
[8] Yang N,Zou D S,Yang M Y,et al.Variations of soil microbial biomass carbon and biological information entropy during revegetation on hillslope land with purple soil.Bulletin of Soil and Water Conservation,2014,34(5):39-43.杨宁,邹冬生,杨满元,等.紫色土丘陵坡地植被恢复过程中土壤微生物生物量碳、微生物熵的变化.水土保持通报,2014,34(5):39-43.
[9] Yu P J,Fan G H,Han K X,et al.Soil quality assessment based on soil microbial biomass carbon and soil enzyme activities.Research of Agricultural Modernization,2018,39(1):163-169.禹朴家,范高华,韩可欣,等.基于土壤微生物生物量碳和酶活性指标的土壤肥力质量评价初探.农业现代化研究,2018,39(1):163-169.
[10] Zeng Q C,Li X,Dong Y H,et al.Soil microbial biomass nitrogen and carbon,water soluble nitrogen and carbon under different arbors forests on the Loess Plateau.Acta Ecological Sinica,2015,35(11):3598-3605.曾全超,李鑫,董扬红,等.黄土高原不同乔木林土壤微生物量碳氮和溶解性碳氮的特征.生态学报,2015,35(11):3598-3605.
[11] Zhang C X,Nan Z B.Research progress of soil microbial biomass in China.Pratacultural Science,2010,27(6):50-57.张成霞,南志标.土壤微生物生物量的研究进展.草业科学,2010,27(6):50-57.
[12] Meng J W,Zuo L,Cai Y J,et al.An analysis of desertification status and its preventive strategies in Zoige County.Journal of Sichuan Forestry Science and Technology,2013,34(4):42-46.蒙嘉文,左林,蔡应君,等.若尔盖县土地沙化现状分析及治理对策研究.四川林业科技,2013,34(4):42-46.
[13] Xu G,Zhao Z Z,Wang Y,et al.Remote- sensing dynamic monitoring of sand- and rock-desertification of the Zoigê Plateau basin,northern Sichuan,China.Geological Bulletin of China,2007,26(8):1048-1055.徐刚,赵志中,王燕,等.川北若尔盖高原盆地沙漠化、岩漠化遥感动态监测研究.地质通报,2007,26(8):1048-1055.
[14] Li S J,Jia C.Formation reasons and restoration strategy of the alpine degraded grassland in northwest of Sichuan.Journal of Sichuan Forestry Science and Technology,2013,34(6):89-92.李守剑,贾程.川西北高寒草地退化成因及恢复对策.四川林业科技,2013,34(6):89-92.
[15] Bowman W D,Theodose T A,Schardt J C,et al.Constraints of nutrient availability on primary production in two alpine tundra communities.Ecology,1993,74(7):2085-2097.
[16] Fenn M E,Poth M A,Aber J D,et al.Nitrogen excess in North American ecosystems:Predisposing factors,ecosystem responses,and management strategies.Ecological Applications,1998,8(3):706-733.
[17] Zong N,Shi P L,Jiang J,et al.Interactive effects of short-term nitrogen enrichment and simulated grazing on ecosystem respiration in an alpine meadow on the Tibetan Plateau.Acta Ecologica Sinica,2013,33(19):6191-6201.宗宁,石培礼,蒋婧,等.短期氮素添加和模拟放牧对青藏高原高寒草甸生态系统呼吸的影响.生态学报,2013,33(19):6191-6201.
[18] Dijkstra F A,Hobbie S E,Reich P B,et al.Divergent effects of elevated CO2,N fertilization,and plant diversity on soil C and N dynamics in a grassland field experiment.Plant & Soil,2005,272(1/2):41-52.
[19] Liu W,Xu W,Han Y,et al.Responses of microbial biomass and respiration of soil to topography,burning,and nitrogen fertilization in a temperate steppe.Biology & Fertility of Soils,2007,44(2):259-268.
[20] He Y L,Qi Y C,Peng Q,et al.Effects of exogenous carbon and nitrogen addition on the key process of carbon cycle in grassland ecosystem:A review.China Environmental Science,2018,38(3):1133-1141.贺云龙,齐玉春,彭琴,等.外源碳氮添加对草地碳循环关键过程的影响.中国环境科学,2018,38(3):1133-1141.
[21] Cheng W Y,Qi D C,Li G Y,et al.Effects of fertilization on grass community diversity and productivity of degraded alpine grassland at Maqu,in south of Gansu Province.Journal of China Agricultural University,2009,14(6):31-36.陈文业,戚登臣,李广宇,等.施肥对甘南高寒草甸退化草地植物群落多样性和生产力的影响.中国农业大学学报,2009,14(6):31-36.
[22] Wang G F,An S Z.Effect of fertilizers on soil fertility,population structure and yield of herbages in degraded grassland.Modern Agricultural Science and Technology,2010,(9):278.王高峰,安沙舟.施肥对退化草甸草地土壤肥力和牧草群落结构及产量的影响.现代农业科技,2010,(9):278.
[23] De K J,Zhang D G,Wang W,et al.Effect of the fertilizers on the Alpine Meadow plant and soil N,P,K,contents.Acta Agrestia Sinica,2014,22(2):299-305.德科加,张德罡,王伟,等.施肥对高寒草甸植物及土壤N,P,K的影响.草地学报,2014,22(2):299-305.
[24] Liu X D,Yin G L,Wu J,et al.Effects of nitrogen addition on the physical properties of soil in an al-pine meadow on the eastern Qinghai-Tibetan Plateau.Acta Prataculturae Sinica,2015,24(10):12-21.刘晓东,尹国丽,武均,等.青藏高原东部高寒草甸草地土壤物理性状对氮元素添加的响应.草业学报,2015,24(10):12-21.
[25] Shui W,Bai J P,Jian X M,et al.Changes in water conservation and soil physicochemical properties during the recovery of desertified grassland in Zoigê,China.Acta Ecologica Sinica,2017,37(1):277-285.税伟,白剑平,简小枚,等.若尔盖沙化草地恢复过程中土壤特性及水源涵养功能.生态学报,2017,37(1):277-285.
[26] Su D X,Zhang Z H,Chen Z Z,et al.Parameters for degradation,sandification and salification of rangelands,GB 19377-2003.Beijing:Standards Press of China,2003:1-3.苏大学,张自和,陈佐忠,等.天然草地退化、沙化、盐渍化的分级标准,GB 19377-2003.北京:中国标准出版社,2003:1-3.
[27] Chinese Academy of Sciences,Institute of Soil Science.Soil nutrient analysis//Soil physical and chemical analysis.Shanghai:Shanghai Scientific & Technical Publishers,1978:15-17.中国科学院南京土壤研究所.土壤养分分析//土壤理化分析.上海:上海科学技术出版社,1978:15-17.
[28] Liu G S.Analysis of soil chemical properties and constant nutrients//Soil physical and chemical analysis and description of soil profiles.Beijing:Standard Press of China,1997:33-43.刘光崧.土壤化学性质及常量养分分析//土壤理化分析与剖面描述.北京:中国标准出版社,1997:33-43.
[29] Mo X X,Ye G F,You S S,et al.Soil microbial biomass and their relationships with soil nutrients in different vegetation restoration patterns on degraded coastal sand.Journal of Fujian College of Forestry,2013,33(2):146-150.莫小香,叶功富,游水生,等.海岸退化沙地不同植被恢复模式的土壤微生物量及其与土壤养分的关系.福建林学院学报,2013,33(2):146-150.
[30] Cai X B,Zhou J,Qian C.Variation of soil microbial activities in alpine steppe different in degradation intensity in the north Tibet Platen.Acta Pedologica Sinica,2008,45(6):1110-1118.蔡晓布,周进,钱成.不同退化程度高寒草原土壤微生物活性变化特征研究.土壤学报,2008,45(6):1110-1118.
[31] Bradley K,Drijber R A,Knops J.Increased N availability in grassland soils modifies their microbial communities and decreases the abundance of arbuscular mycorrhizal fungi.Soil Biology & Biochemistry,2006,38(7):1583-1595.
[32] Zhao L.Effects of nitrogen addition on the soil nutrient and microbial biomass in root zone of Medicago sativa.Reasearch of Soil and Water Conservation,2014,21(6):35-40.赵莉.氮添加对紫花苜蓿根区土壤养分及土壤微生物量的影响.水土保持研究,2014,21(6):35-40.
[33] He Y T,Dong Y S,Qi Y C,et al.Advances in researches on soil microbial biomass of grassland ecosystems and its influencing factors.Progress in Geography,2010,29(11):1350-1359.何亚婷,董云社,齐玉春,等.草地生态系统土壤微生物量及其影响因子研究进展.地理科学进展,2010,29(11):1350-1359.
[34] Lin L,Wu Y N,Kenji T,et al.Variation of soil physicochemical and microbial properties in degraded steppes in Hulunbeir of China.Chinese Journal of Applied Ecology,2013,24(12):3407-3414.林璐,乌云娜,田村宪司,等.呼伦贝尔典型退化草原土壤理化与微生物性状.应用生态学报,2013,24(12):3407-3414.
[35] Yang Z A.A study on the responses of vegetation-soil system to grazing and nitrogen addition in an alpine meadow of Qinghai-Tibetan plateau,China.Yangling:Northwest Agricultural and Forestry University,2017.杨振安.青藏高原高寒草甸植被土壤系统对放牧和氮添加的响应研究.杨凌:西北农林科技大学,2017.
[36] Liu J S,Dai J,Liu Y,et al.Effects of excessive nitrogen fertilization on soil organic carbon and nitrogen and nitrogen supply capacity in dryland.Journal of Plant Nutrition and Fertilizer,2015,21(1):112-120.刘金山,戴健,刘洋,等.过量施氮对旱地土壤碳、氮及供氮能力的影响.植物营养与肥料学报,2015,21(1):112-120.
[37] Liu W Q,Liu B Y,Wang J,et al.Response of soil microbial communities to moss cover and nitrogen addition.Acta Ecologica Sinica,2010,30(7):1691-1698.刘蔚秋,刘滨扬,王江,等.不同环境条件下土壤微生物对模拟大气氮沉降的响应.生态学报,2010,30(7):1691-1698.
[38] Wang C T,Wang G X,Liu W,et al.Effects of fertilization gradients on plant community structure and soil characteristics in alpine meadow.Acta Ecologica Sinica,2013,33(10):3103-3113.王长庭,王根绪,刘伟,等.施肥梯度对高寒草甸群落结构、功能和土壤质量的影响.生态学报,2013,33(10):3103-3113.
[39] Wu J S,Lin Q M,Huang Q Y,et al.Soil microbial biomass determination method and its application.Beijing:China Meteorological Press,2006:71-75.吴金水,林启美,黄巧云,等.土壤微生物生物量测定方法及其应用.北京:气象出版社,2006:71-75.
[40] Wang H H,Ren T B,Yuan Y,et al.Combined application of biomass charcoal and nitrogen fertilizer:Effects on microbial biomass C,microbial biomass N and C/N in tobacco soil.Chinese Agricultural Science Bulletin,2017,33(12):52-57.王欢欢,任天宝,元野,等.生物质炭与氮肥配施对植烟土壤微生物量碳、氮和碳氮比的影响.中国农学通报,2017,33(12):52-57.
[41] Li F,Liu Z H,Jia T H,et al.Functional diversity of soil microbial community carbon metabolism with the degradation and restoration of alpine wetlands and meadows.Acta Ecologica Sinica,2018,38(17):6006-6015.李飞,刘振恒,贾甜华,等.高寒湿地和草甸退化及恢复对土壤微生物碳代谢功能多样性的影响.生态学报,2018,38(17):6006-6015.
[42] Zhu R F,Tang F L,Liu J L,et al.Response of soil microbial biomass carbon and nitrogen on a short-term fertilizing N in Leymus chinensis meadow.Acta Agrestia Sinica,2016,24(3):553-558.朱瑞芬,唐凤兰,刘杰淋,等.羊草草甸草原土壤微生物生物量碳氮对短期施氮的响应.草地学报,2016,24(3):553-558.
[43] Dai D,Zi H B,Yang Y F,et al.Responses of soil microbial functional diversity to nitrogen addition in an alpine meadow on Northwestern Plateau of Sichuan Province.Research of Soil and Water Conservation,2018,25(2):137-144.代迪,字洪标,杨有芳,等.高寒草甸土壤微生物功能多样性对氮肥添加的响应.水土保持研究,2018,25(2):137-144.
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