冬瓜高通量转录组测序及分析
|
摘要
【目的】获得冬瓜转录组序列、遗传变异等信息,从中挖掘冬瓜基因数据及SSR分子标记,为冬瓜后续研究提供数据支撑。【方法】以冬瓜嫩叶为材料,利用Illumina HiSeq~(TM)2000技术对冬瓜进行转录组测序,构建数据库从中获得干净序列。经De novo拼接组装后,将获得的单基因簇(Unigene)数据在非冗余蛋白数据库(nonredundant protein database,Nr)、蛋白质序列数据库(Swiss Prot protein database,Swiss Prot)、基因本体论数据库(gene ontology,GO)、蛋白质真核同源数据库(eukaryotic orthologous groups,KOG)、东京基因与基金组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)、蛋白质家族域数据库(protein families database,Pfam)6个公共数据库中进行比对,最终得到冬瓜单基因簇注释信息。利用MISA软件对转录组单基因簇进行搜索,获得单基因簇中的SSR位点。【结果】从冬瓜嫩叶中得到62 021 032条高品质序列,组装后获得40 611条单基因簇,平均长度955 bp。将所有单基因簇在Nr和Swiss Prot数据库中进行比对,结果分别比对到27 474及19 573条单基因簇;在GO数据库中,所注释到的10 659条单基因簇分别匹配到生物功能、分子功能和细胞组分3个本体的47个功能组中;与KOG数据库进行注释比对,根据其功能将注释到的单基因簇划分为25类;KEGG数据库比对注释到10 799条冬瓜的单基因簇,可分为5个大类、19个亚类、125条代谢途径;在Pfam数据库中比对到17 990条单基因簇,分属于369个类群。SSR位点搜索发现,有5 086条单基因簇包含SSR序列,获得5 474个SSR位点。【结论】利用高通量测序获得大量冬瓜转录组信息,有助于从分子水平对冬瓜进行深入研究。
【Objective】 The transcriptional sequence,genetic variation and other information of Benincasa hispida were obtained and genetic data and SSR markers were extracted to improve researches on Benincasa hispida.【Method】 The Illumina HiSeq~(TM)2000 technology was used for the transcription sequencing of Benincasa hispida tender leaves, and a transcriptome database was built to obtain clean reads.The annotation information of the Unigenes was obtained by De novo splicing assembly and compared with public database(Includes Nr,Swiss Prot,GO,KOG,KEGG,and Pfam).MISA software was used to search the transcription group Unigenes and obtain the SSR sites in Unigenes.【Result】 A total of 62 021 032 reads fragments were generated by sequencing.The reads gave 40 611 Unigenes with an average length of 955 bp.A total of 27 474 and 19 573 Unigenes were annotated against the Nr and Swiss Prot databases,respectively.Gene ontology analysis revealed that annotated 10 659 unigenes were grouped into 47 different categories in terms of biological function,molecular function and cellular component.Unigenes in the transcriptome of Benincasa hispida were divided into 25 classes according to the function comparing with the KOG database.Moreover,10 799 unigenes were annotated to 125 KEGG pathway and broadly divided into 5 categories of 19 branches,and 17 990 unigenes were annotated against Pfam database and formed 369 groups.A total of 5 474 SSRs were identified from the sequence of transcription,distributed in 5 086 unigenes.【Conclusion】 Large amount of transcriptome information was obtained by high-throughput sequencing,which was helpful for further study of Benincasa hispida at molecular level.
【Objective】 The transcriptional sequence,genetic variation and other information of Benincasa hispida were obtained and genetic data and SSR markers were extracted to improve researches on Benincasa hispida.【Method】 The Illumina HiSeq~(TM)2000 technology was used for the transcription sequencing of Benincasa hispida tender leaves, and a transcriptome database was built to obtain clean reads.The annotation information of the Unigenes was obtained by De novo splicing assembly and compared with public database(Includes Nr,Swiss Prot,GO,KOG,KEGG,and Pfam).MISA software was used to search the transcription group Unigenes and obtain the SSR sites in Unigenes.【Result】 A total of 62 021 032 reads fragments were generated by sequencing.The reads gave 40 611 Unigenes with an average length of 955 bp.A total of 27 474 and 19 573 Unigenes were annotated against the Nr and Swiss Prot databases,respectively.Gene ontology analysis revealed that annotated 10 659 unigenes were grouped into 47 different categories in terms of biological function,molecular function and cellular component.Unigenes in the transcriptome of Benincasa hispida were divided into 25 classes according to the function comparing with the KOG database.Moreover,10 799 unigenes were annotated to 125 KEGG pathway and broadly divided into 5 categories of 19 branches,and 17 990 unigenes were annotated against Pfam database and formed 369 groups.A total of 5 474 SSRs were identified from the sequence of transcription,distributed in 5 086 unigenes.【Conclusion】 Large amount of transcriptome information was obtained by high-throughput sequencing,which was helpful for further study of Benincasa hispida at molecular level.
引文
[1] Morton J F.The wax gourd,a year-round Florida vegetable with unusual keeping quality [C]//Florida State Horticultural Society Proceeding.Miami:Florida State Horticultural Society,1971:104-109.
[2] 张帅中,梁雪.冬瓜皮药用价值及综合利用研究进展 [J].现代农业科技,2016(9):286-288.Zhang S Z,Liang X.Research progress on officinal value and comprehensive utilization of wax gourd peel [J].Modern Agricultural Sciences and Technology,2016(9):286-288.
[3] Huang H Y,Huang J J,Tso T K,et al.Antioxidant and angiotension-converting enzyme inhibition capacities of various parts of Benincasa hispida (wax gourd) [J].Molecular Nutrition,2004,48(3):230-233.
[4] 旷碧峰,唐锷,余席茂,等.冬瓜搭架栽培技术 [J].上海蔬菜,2016(5):40-42.Kuang B F,Tang E,Yu X M,et al.Technology of wax melon viaduct cultivation [J].Shanghai Vegetables,2016(5):40-42.
[5] 骆焕彬,易文红,卢文佳,等.特黑杂交黑皮冬瓜的特征特性及高产栽培技术 [J].广东农业科学,2007(8):22-23.Luo H B,Yi W H,Lu W J,et al.Characteristic and high yield cultivation techniques of extra-black hybrid black gourd [J].Guangdong Agricultural Science,2007(8):22-23.
[6] 张淑瑛.冬瓜的美容及药用价值 [J].中国民间疗法,1996(4):49.Zhang S Y.Beauty and medicinal value of wax gourd [J].China’s Naturopathy,1996(4):49.
[7] 夏明,阮叶萍.冬瓜提取物抗小鼠胃溃疡活性研究 [J].食品科学,2005(4):243-246.Xia M,Ruan Y P.Study on anti-gastric ulcer activity of winter melon extract in mice [J].Food Science,2005(4):243-246.
[8] Grover J K,Adiga G,Vats V,et al.Extracts of Benincasa hispida prevent development of experimental ulcers [J].Journal of Ethnopharmacology,2001,78:159-164.
[9] 车双辉.冬瓜抗胃溃疡活性组分的筛选 [D].杭州:浙江工商大学,2004.Che S H.Screening of active components of winter melon against gastric ulcer [D].Hangzhou:Zhejiang Gongshang University,2004.
[10] 邹宇晓,徐玉娟,廖森泰,等.冬瓜的营养价值及其综合利用研究进展 [J].中国果菜,2006(5):46-47.Zou Y X,Xu Y J,Liao S T,et al.Research progress on nutrition value and comprehensive utilization of winter melon [J].Chinese Fruit and Vegetable,2006(5):46-47.
[11] Wu C M,Liou S E,Chang Y H,et al.Volatile compounds of the wax gourd (Benincasa hispida Cogn) and a wax gourd beverage [J].Journal of Food Science,1987,52(1):132-134.
[12] Pandey S,Kumar S,Mishra U,et al.Genetic diversity in Indian ash gourd (Benincasa hispida) accessions as revealed by quantitative traits and RAPD markers [J].HortScience,2008,118:80-86.
[13] Jiang B,Xie D,Liu W,et al.De novo assembly and characterization of the transcriptome,and development of SSR markers in wax gourd (Benicasa hispida) [J].PLoS One,2013,8(8):e71054.
[14] Jiang B,Liu W R,Xie D,et al.High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing [J].BMC Genomics,2015,16:1035.
[15] Costa V,Angelini C,Feis D I,et al.Uncovering the complexity of transcriptomes with RNA-Seq [J].Biomedicine and Biotechnology,2010(1):853-916.
[16] Morozova O,Marra M A.Applications of next-generation sequencing technologies in functional genomics [J].Genomics,2008,92:255-264.
[17] Wang Z,Gerstein M,Snyder M.RNA-Seq:a revolutionary tool for transcriptomics [J].Nat Rev Genet,2009,10:57-63.
[18] Cao J,Schneeberger K,Ossowski S,et al.Whole-genome sequencing of multiple Arabidopsis thaliana populations [J].Nature Genet,2011,43:956-963.
[19] Zhan J,Thakare D,Ma C,et al.RNA sequencing of laser-capture microdissected compartments of the maize kernel identifies regulatory modules associated with endosperm cell differentiation [J].Plant Cell,2015,27:513-531.
[20] Zhang X,Song Z Q,Liu T,et al.De novo assembly and comparative transcriptome analysis provide insightinto lysine biosynthesis in Toona sinensis Roem [J].Journal of Genomics,2016(3):1-9.
[21] 张少平,邱珊莲,郑云云,等.紫色黄秋葵转录组功能基因测序及分析 [J].核农学报,2017,31(4):643-653.Zhang S P,Qiu S L,Zheng Y Y,et al.Functional gene sequencing and analysis of the transcription group of purple okra [J].Nuclear Agricultural Sciences,2017,31(4):643-653.
[22] Altschul S F,Gish W,Miller W,et al.Basic local alignment search tool [J].Molecular Biology,1990,215(3):403-410.
[23] Cui J,You C,Zhu E,et al.Feedback regulation of DYT1 by interactions with downstream bHLH factors promotes DYT1 nuclear localization and anther development [J].Plant Cell,2016,28(5):1078.
[24] 何洁,顾秀容,魏春华,等.西瓜bHLH转录因子家族基因的鉴定及其在非生物胁迫下的表达分析 [J].园艺学报,2016,43(2):281-294.He J,Gu X R,Wei C H,et al.Nuclear agricultural sciences,dentification and expression analysis under abiotic stresses of the bHLH transcription factor gene family in watermelon [J].Acta Horticultural Sinica,2016,43(2):281-294.
[25] Riechmann J L,Heard J,Martin G,et al.Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes [J].Science,2000,290:2105-2110.
[26] 刘建光,王永强,张寒霜,等.ERF转录因子在植物抗逆境胁迫的研究进展 [J].华北农学报,2013,28(S1):214-218.Liu J G,Wang Y Q,Zhang H S,et al.Research progress of ERF transcription factors in plant biotic and abiotic stress responses [J].Acta Agriculturae Boreali Sinica,2013,28(S1):214-218.
[27] 张金彪,周碧青,雷永程,等.植物源POD和CAT的筛选及其与重金属的相互作用 [J].福建农林大学学报(自然科学版),2009(3):295-300.Zhang J B,Zhou B Q,Lei Y C,et al.Screening of botanical POD and CAT and their interaction with heavy metals [J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2009(3):295-300.
[28] 黄建华.冬瓜皮汤治疗急性荨麻疹50例 [J].山东中医杂志,1995(6):252-253.Huang J H.Fifty cases of acute urticaria were treated by donggua skin decoction [J].Shandong Journal of Traditional Chinese Medicine,1995(6):252-253.
[29] 陈晓娟,溪茜,黄兆富.中药饮剂对大鼠降压和利尿的效果 [J].第四军医大学学报,2000(12):S237-S238.Chen X J,Xi Q,Huang Z F.Effects of traditional Chinese medicine decoction on hypertension and diuresis in rats [J].Journal of the Fourth Military Medical University,2000(12):S237-S238.
[30] Gu M,Fan S,Liu G,et al.Extract of wax gourd peel prevents high-fat diet-induced hyperlipidemia in C57BL/6 mice via the inhibition of the PPARγ pathway [J].Evidence-Based Complementary and Alternative Medicine,2013(10):3-4.
[31] 魏利斌,苗红梅,张海洋.芝麻发育转录组分析 [J].中国农业科学,2012(7):1246-1256.Wei L B,Miao H M,Zhang H Y.Transcriptomic analysis of sesame development [J].Scientia Agricultura Sinica,2012(7):1246-1256.
[32] Gonzalez A,Zhao M,Leavitt J M,et al.Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings [J].Plant Journal,2008,53(5):814-827.
[33] Jung K H,Han M J,Lee Y S,et al.Rice Undeveloped Tapetum1 is a major regulator of early tapetum development [J].Plant Cell,2005,17(10):2705-2722.
[34] 范中奇,邝健飞,陆旺金,等.转录因子调控果实成熟和衰老机制研究进展 [J].园艺学报,2015,42(9):1649-1663.Fan Z Q,Kuang J F,Lu W J,et al.Advances in research of the mechanism of transcription factors involving in regulating fruit ripening and senescence [J].Acta Horticultural Sinica,2015,42(9):1649-1663.
[35] 孙丽芳,邢少辰,张君,等.转录因子在植物进化和抗逆中的作用 [J].基因组学与应用生物学,2009,28(3):569-577.Sun L F,Xing S C,Zhang J,et al.Function of the transcription factors in plant domestication and stress resistance [J].Genomics and Applied Biology,2009,28(3):569-577.
[36] 王娜,张振葆,黄凤珠,等.WRKY转录因子参与植物非生物胁迫应答的研究进展 [J].核农学报,2014,28(10):1819-1827.Wang N,Zhang Z B,Huang F Z,et al.Research progress on transcriptional factors involved in plant abiotic stress response [J].Nuclear Agricultural Sciences,2014,28(10):1819-1827.
[37] 朱海生,张前荣,刘建汀,等.丝瓜转录组SSR位点分析及其分子标记开发 [J].中国细胞生物学学报,2016,38(9):1118-1127.Zhu H S,Zhang Q Q,Liu J T,et al.Analysis on SSR information in transcriptome and development of molecular markers in Luffa cylindrica [J].Chinese Journal of Cell Biology,2016,38(9):1118-1127.
[38] 魏秀清,许玲,章希娟,等.莲雾转录组SSR信息分析及其分子标记开发 [J].园艺学报,2018,45(3):541-551.Wei X Q,Xu L,Zhang X J,et al.Analysis on SSR information in transcriptome and development of molecular markers in wax apple [J].Acta Horticulturae Sinica,2018,45(3):541-551.
[39] 王洋洋,单文琪,徐文龙,等.印度南瓜转录组SSR信息分析及其多态性研究 [J].园艺学报,2016,43(3):578-586.Wang Y Y,Shan W Q,Xu W L,et al.Analysis on SSR information in transcriptome and the polymorphism of Cucurbita maxima [J].Acta Horticulturae Sinica,2016,43(3):578-586.
[40] 李永平,刘建汀,陈敏氡,等.利用黄秋葵转录组信息挖掘SSR标记及用于种质分析 [J].园艺学报,2018,45(3):579-590.Li Y P,Liu J T,Chen M D,et al.SSR markers excavation and germplasm analysis using the transcriptome information of Hibiscus esculentus [J].Acta Horticulturae Sinica,2018,45(3):579-590.
[41] Zhai L,Xu L,Wang Y,et al.Novel and useful genic-SSR markers from de novo transcriptome sequencing of radish (Raphanus sativus L.) [J].Molecular Breed,2014,33(3):611-624.
[42] 程云清,张丽娜,赵永斌,等.平欧杂交榛转录组中SSR信息分析和引物筛选 [J].园艺学报,2018,45(1):139-148.Cheng Y Q,Zhang L N,Zhao Y B,et al.Analysis of SSR markers information and primer selection from transcriptome sequence of Hybrid Hazelnut Corylus heterophylla×C.avellane [J].Acta Horticulturae Sinica,2018,45(1):139-148.
[43] 李荣华,王直亮,陈静芳,等.菜薹转录组中SSR信息与可用性分析 [J].园艺学报,2016,43(9):1816-1824.Liu R H,Wang Z L,Chen J F,et al.Analysis of SSR information in transcriptome and their usability in Flowering Chinese Cabbage [J].Acta Horticulturae Sinica,2016,43(9):1816-1824.
[44] Morgante M,Hanafey M,Powell W.Microsatellites are preferentially associated with nonrepetitive DNA in plant genomes [J].Nature Genetics,2002,30(2):194-200.
[2] 张帅中,梁雪.冬瓜皮药用价值及综合利用研究进展 [J].现代农业科技,2016(9):286-288.Zhang S Z,Liang X.Research progress on officinal value and comprehensive utilization of wax gourd peel [J].Modern Agricultural Sciences and Technology,2016(9):286-288.
[3] Huang H Y,Huang J J,Tso T K,et al.Antioxidant and angiotension-converting enzyme inhibition capacities of various parts of Benincasa hispida (wax gourd) [J].Molecular Nutrition,2004,48(3):230-233.
[4] 旷碧峰,唐锷,余席茂,等.冬瓜搭架栽培技术 [J].上海蔬菜,2016(5):40-42.Kuang B F,Tang E,Yu X M,et al.Technology of wax melon viaduct cultivation [J].Shanghai Vegetables,2016(5):40-42.
[5] 骆焕彬,易文红,卢文佳,等.特黑杂交黑皮冬瓜的特征特性及高产栽培技术 [J].广东农业科学,2007(8):22-23.Luo H B,Yi W H,Lu W J,et al.Characteristic and high yield cultivation techniques of extra-black hybrid black gourd [J].Guangdong Agricultural Science,2007(8):22-23.
[6] 张淑瑛.冬瓜的美容及药用价值 [J].中国民间疗法,1996(4):49.Zhang S Y.Beauty and medicinal value of wax gourd [J].China’s Naturopathy,1996(4):49.
[7] 夏明,阮叶萍.冬瓜提取物抗小鼠胃溃疡活性研究 [J].食品科学,2005(4):243-246.Xia M,Ruan Y P.Study on anti-gastric ulcer activity of winter melon extract in mice [J].Food Science,2005(4):243-246.
[8] Grover J K,Adiga G,Vats V,et al.Extracts of Benincasa hispida prevent development of experimental ulcers [J].Journal of Ethnopharmacology,2001,78:159-164.
[9] 车双辉.冬瓜抗胃溃疡活性组分的筛选 [D].杭州:浙江工商大学,2004.Che S H.Screening of active components of winter melon against gastric ulcer [D].Hangzhou:Zhejiang Gongshang University,2004.
[10] 邹宇晓,徐玉娟,廖森泰,等.冬瓜的营养价值及其综合利用研究进展 [J].中国果菜,2006(5):46-47.Zou Y X,Xu Y J,Liao S T,et al.Research progress on nutrition value and comprehensive utilization of winter melon [J].Chinese Fruit and Vegetable,2006(5):46-47.
[11] Wu C M,Liou S E,Chang Y H,et al.Volatile compounds of the wax gourd (Benincasa hispida Cogn) and a wax gourd beverage [J].Journal of Food Science,1987,52(1):132-134.
[12] Pandey S,Kumar S,Mishra U,et al.Genetic diversity in Indian ash gourd (Benincasa hispida) accessions as revealed by quantitative traits and RAPD markers [J].HortScience,2008,118:80-86.
[13] Jiang B,Xie D,Liu W,et al.De novo assembly and characterization of the transcriptome,and development of SSR markers in wax gourd (Benicasa hispida) [J].PLoS One,2013,8(8):e71054.
[14] Jiang B,Liu W R,Xie D,et al.High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing [J].BMC Genomics,2015,16:1035.
[15] Costa V,Angelini C,Feis D I,et al.Uncovering the complexity of transcriptomes with RNA-Seq [J].Biomedicine and Biotechnology,2010(1):853-916.
[16] Morozova O,Marra M A.Applications of next-generation sequencing technologies in functional genomics [J].Genomics,2008,92:255-264.
[17] Wang Z,Gerstein M,Snyder M.RNA-Seq:a revolutionary tool for transcriptomics [J].Nat Rev Genet,2009,10:57-63.
[18] Cao J,Schneeberger K,Ossowski S,et al.Whole-genome sequencing of multiple Arabidopsis thaliana populations [J].Nature Genet,2011,43:956-963.
[19] Zhan J,Thakare D,Ma C,et al.RNA sequencing of laser-capture microdissected compartments of the maize kernel identifies regulatory modules associated with endosperm cell differentiation [J].Plant Cell,2015,27:513-531.
[20] Zhang X,Song Z Q,Liu T,et al.De novo assembly and comparative transcriptome analysis provide insightinto lysine biosynthesis in Toona sinensis Roem [J].Journal of Genomics,2016(3):1-9.
[21] 张少平,邱珊莲,郑云云,等.紫色黄秋葵转录组功能基因测序及分析 [J].核农学报,2017,31(4):643-653.Zhang S P,Qiu S L,Zheng Y Y,et al.Functional gene sequencing and analysis of the transcription group of purple okra [J].Nuclear Agricultural Sciences,2017,31(4):643-653.
[22] Altschul S F,Gish W,Miller W,et al.Basic local alignment search tool [J].Molecular Biology,1990,215(3):403-410.
[23] Cui J,You C,Zhu E,et al.Feedback regulation of DYT1 by interactions with downstream bHLH factors promotes DYT1 nuclear localization and anther development [J].Plant Cell,2016,28(5):1078.
[24] 何洁,顾秀容,魏春华,等.西瓜bHLH转录因子家族基因的鉴定及其在非生物胁迫下的表达分析 [J].园艺学报,2016,43(2):281-294.He J,Gu X R,Wei C H,et al.Nuclear agricultural sciences,dentification and expression analysis under abiotic stresses of the bHLH transcription factor gene family in watermelon [J].Acta Horticultural Sinica,2016,43(2):281-294.
[25] Riechmann J L,Heard J,Martin G,et al.Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes [J].Science,2000,290:2105-2110.
[26] 刘建光,王永强,张寒霜,等.ERF转录因子在植物抗逆境胁迫的研究进展 [J].华北农学报,2013,28(S1):214-218.Liu J G,Wang Y Q,Zhang H S,et al.Research progress of ERF transcription factors in plant biotic and abiotic stress responses [J].Acta Agriculturae Boreali Sinica,2013,28(S1):214-218.
[27] 张金彪,周碧青,雷永程,等.植物源POD和CAT的筛选及其与重金属的相互作用 [J].福建农林大学学报(自然科学版),2009(3):295-300.Zhang J B,Zhou B Q,Lei Y C,et al.Screening of botanical POD and CAT and their interaction with heavy metals [J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2009(3):295-300.
[28] 黄建华.冬瓜皮汤治疗急性荨麻疹50例 [J].山东中医杂志,1995(6):252-253.Huang J H.Fifty cases of acute urticaria were treated by donggua skin decoction [J].Shandong Journal of Traditional Chinese Medicine,1995(6):252-253.
[29] 陈晓娟,溪茜,黄兆富.中药饮剂对大鼠降压和利尿的效果 [J].第四军医大学学报,2000(12):S237-S238.Chen X J,Xi Q,Huang Z F.Effects of traditional Chinese medicine decoction on hypertension and diuresis in rats [J].Journal of the Fourth Military Medical University,2000(12):S237-S238.
[30] Gu M,Fan S,Liu G,et al.Extract of wax gourd peel prevents high-fat diet-induced hyperlipidemia in C57BL/6 mice via the inhibition of the PPARγ pathway [J].Evidence-Based Complementary and Alternative Medicine,2013(10):3-4.
[31] 魏利斌,苗红梅,张海洋.芝麻发育转录组分析 [J].中国农业科学,2012(7):1246-1256.Wei L B,Miao H M,Zhang H Y.Transcriptomic analysis of sesame development [J].Scientia Agricultura Sinica,2012(7):1246-1256.
[32] Gonzalez A,Zhao M,Leavitt J M,et al.Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings [J].Plant Journal,2008,53(5):814-827.
[33] Jung K H,Han M J,Lee Y S,et al.Rice Undeveloped Tapetum1 is a major regulator of early tapetum development [J].Plant Cell,2005,17(10):2705-2722.
[34] 范中奇,邝健飞,陆旺金,等.转录因子调控果实成熟和衰老机制研究进展 [J].园艺学报,2015,42(9):1649-1663.Fan Z Q,Kuang J F,Lu W J,et al.Advances in research of the mechanism of transcription factors involving in regulating fruit ripening and senescence [J].Acta Horticultural Sinica,2015,42(9):1649-1663.
[35] 孙丽芳,邢少辰,张君,等.转录因子在植物进化和抗逆中的作用 [J].基因组学与应用生物学,2009,28(3):569-577.Sun L F,Xing S C,Zhang J,et al.Function of the transcription factors in plant domestication and stress resistance [J].Genomics and Applied Biology,2009,28(3):569-577.
[36] 王娜,张振葆,黄凤珠,等.WRKY转录因子参与植物非生物胁迫应答的研究进展 [J].核农学报,2014,28(10):1819-1827.Wang N,Zhang Z B,Huang F Z,et al.Research progress on transcriptional factors involved in plant abiotic stress response [J].Nuclear Agricultural Sciences,2014,28(10):1819-1827.
[37] 朱海生,张前荣,刘建汀,等.丝瓜转录组SSR位点分析及其分子标记开发 [J].中国细胞生物学学报,2016,38(9):1118-1127.Zhu H S,Zhang Q Q,Liu J T,et al.Analysis on SSR information in transcriptome and development of molecular markers in Luffa cylindrica [J].Chinese Journal of Cell Biology,2016,38(9):1118-1127.
[38] 魏秀清,许玲,章希娟,等.莲雾转录组SSR信息分析及其分子标记开发 [J].园艺学报,2018,45(3):541-551.Wei X Q,Xu L,Zhang X J,et al.Analysis on SSR information in transcriptome and development of molecular markers in wax apple [J].Acta Horticulturae Sinica,2018,45(3):541-551.
[39] 王洋洋,单文琪,徐文龙,等.印度南瓜转录组SSR信息分析及其多态性研究 [J].园艺学报,2016,43(3):578-586.Wang Y Y,Shan W Q,Xu W L,et al.Analysis on SSR information in transcriptome and the polymorphism of Cucurbita maxima [J].Acta Horticulturae Sinica,2016,43(3):578-586.
[40] 李永平,刘建汀,陈敏氡,等.利用黄秋葵转录组信息挖掘SSR标记及用于种质分析 [J].园艺学报,2018,45(3):579-590.Li Y P,Liu J T,Chen M D,et al.SSR markers excavation and germplasm analysis using the transcriptome information of Hibiscus esculentus [J].Acta Horticulturae Sinica,2018,45(3):579-590.
[41] Zhai L,Xu L,Wang Y,et al.Novel and useful genic-SSR markers from de novo transcriptome sequencing of radish (Raphanus sativus L.) [J].Molecular Breed,2014,33(3):611-624.
[42] 程云清,张丽娜,赵永斌,等.平欧杂交榛转录组中SSR信息分析和引物筛选 [J].园艺学报,2018,45(1):139-148.Cheng Y Q,Zhang L N,Zhao Y B,et al.Analysis of SSR markers information and primer selection from transcriptome sequence of Hybrid Hazelnut Corylus heterophylla×C.avellane [J].Acta Horticulturae Sinica,2018,45(1):139-148.
[43] 李荣华,王直亮,陈静芳,等.菜薹转录组中SSR信息与可用性分析 [J].园艺学报,2016,43(9):1816-1824.Liu R H,Wang Z L,Chen J F,et al.Analysis of SSR information in transcriptome and their usability in Flowering Chinese Cabbage [J].Acta Horticulturae Sinica,2016,43(9):1816-1824.
[44] Morgante M,Hanafey M,Powell W.Microsatellites are preferentially associated with nonrepetitive DNA in plant genomes [J].Nature Genetics,2002,30(2):194-200.