芒柄花黄素对实验小鼠免疫功能的影响

详细信息    查看全文 | 推荐本文 |

英文篇名：Effects of Formononetin on the Immune Functions of Experimental Mice 作者：杨雅静 ; 裴银辉 ; 李琳 英文作者：YANG Ya-jing;PEI Yin-hui;LI Lin;School of Basic Medical Sciences, North China University of Science and Technology; 关键词：芒柄花黄素 ; 脾细胞 ; 固有免疫 英文关键词：Formononetin;;Spleen cell;;Innate immune 中文刊名：安徽农业科学 英文刊名：Journal of Anhui Agricultural Sciences 机构：华北理工大学基础医学院; 出版日期：2019-05-27 11:48 出版单位：安徽农业科学 年：2019 期：10 语种：中文; 页：94-96+108 页数：4 CN：34-1076/S ISSN：0517-6611 分类号：R285.5

摘要

[目的]研究芒柄花黄素对实验小鼠免疫功能的影响。[方法]取SPF级雄性昆明小鼠40只,随机分为低、中、高剂量芒柄花黄素试验组和对照组,连续注射芒柄花黄素4周后检测各组小鼠巨噬细胞的吞噬功能、小鼠脾细胞凋亡率及转化率、小鼠血清中溶菌酶、IgG、IFN-γ及IL-4的含量。[结果]中、高剂量芒柄花黄素组小鼠巨噬细胞吞噬率及血清溶菌酶含量高于对照组及低剂量组,差异显著(P<0.05)。低、中、高剂量芒柄花黄素组小鼠脾细胞凋亡率低于对照组,差异显著(P<0.05)。中、高剂量组小鼠脾细胞转化率高于低剂量组及对照组,差异显著(P<0.05)。中、高剂量芒柄花黄素组小鼠血清IgG含量高于对照组及低剂量组,差异显著(P<0.05)。低、中、高剂量芒柄花黄素组小鼠血清中IL-4含量高于对照组,差异显著(P<0.05),而小鼠血清中IFN-γ含量在各组间差异不显著(P>0.05)。[结论]芒柄花黄素可以提高小鼠的固有免疫功能,抑制脾细胞凋亡并提高其转化率。
        [Objective] To study the effects of formononetin on the immune functions of mice. [Method] 40 male Kunming mice(SPF class) were divided into 4 groups, including low dose group, middle dose group, high dose group and control group. The phagocytic function of macrophages,the apoptosis rate and conversion rate of spleen cells,the contents of lysozyme, IgG, IFN-γ, IL-4 in the serum of mice were detected after injecting formononetin for four weeks continuously. [Result]The phagocytosis rate of macrophage and serum lysozyme content of mice in the medium-dose group and high-dose group of formononetin were higher than those in the control group and low-dose group, and the difference was significant(P<0.05). The apoptosis rate of mice spleen cells in the low-dose group, medium-dose group and high-dose group of formononetin was lower than that in the control group, and the difference was significant(P<0.05).The transformation rate of spleen cells in the medium-dose group and hig-dose group was higher than that in the low-dose group and the control group, and the difference was significant(P<0.05). The serum IgG content of mice in the medium-dose group and high-dose group of formononetin was higher than that in the control group and the low dose group, and the difference was significant(P<0.05). The content of IL-4 in the serum of mice in low-dose group, medium-dose group and high-dose group of formononetin were higher than those of the control group, and the differences were significant(P<0.05), while the content of IFN-γ in the serum of mice were not significant among different groups(P>0.05). [Conclusion] Formononetin can improve the innate immune function, inhibit the apoptosis of spleen cells and improve their transformation rate in mice.

引文

[1]姜慈静,吴秋慧,任倩瑶,等.芒柄花黄素对大鼠局部脑缺血再灌注损伤保护作用的初步研究[J].中国医药导报,2017,14(32):8-10,14.
    [2]PARK S,BAZER F W,LIM W,et al.The O-methylated isoflavone,formononetin,inhibits human ovarian cancer cell proliferation by sub G0/G1cell phase arrest through PI3K/AKT and ERK1/2 inactivation[J].J Cell Biochem,2018,119(9):7377-7387.
    [3]张幸,梁梅花,黄文君,等.芒柄花黄素诱导膀胱癌细胞凋亡作用[J].中国公共卫生,2015,31(3):314-317.
    [4]董陈诚,钟漓,张广钰,等.芒柄花黄素对人胃癌细胞MKN-45荷瘤裸鼠模型的抑制作用及机制研究[J].重庆医学,2016,45(32):4482-4483,4486.
    [5]梁馨元,韦姗姗,李礼顺,等.芒柄花黄素对小鼠体内SOD、MDA、GSH-Px和BUN的影响[J].湖北科技学院学报(医学版),2017,31(3):188-190.
    [6]王昌明,刘婷,李鹤,等.铁箍散乳膏对大鼠皮肤疮疡的药效学研究[J].中南药学,2015,13(2):136-140.
    [7]田强,冯香芝,杨丽敏,等.鹅脾混合多肽对小鼠非特异性免疫功能的影响[J].医药导报,2015,34(2):158-161.
    [8]杨朝晔,秦红兵,成海龙,等.麋鹿角醇提液对衰老小鼠行为及免疫功能的影响[J].中华中医药杂志,2010,25(2):221-225.
    [13]华慧,张波,李向阳,等.华支睾吸虫脂肪酸结合蛋白Cs FABP对巨噬细胞免疫功能的影响[J].中国病原生物学杂志,2017,12(11):1058-1061,1065.
    [14]吴婷,周武雄.肿瘤微环境中肿瘤相关巨噬细胞的研究进展[J].细胞与分子免疫学杂志,2016,32(2):265-267,271.
    [15]沈芃.溶菌酶抗菌作用及其在防控医院感染中的应用[J].中国消毒学杂志,2014,31(10):1089-1091.
    [16]沙爱龙,孟庆艳,郝海燕.小新塔花水提物对小鼠镇静催眠及免疫功能的影响[J].现代食品科技,2016,32(5):22-26,39.
    [17]张振宇,黄衍松,李伟冰,等.慢性乙型肝炎中医证型与患者细胞免疫功能关系研究[J].中西医结合肝病杂志,2018,28(1):8-10.
    [18]蔡琨,王晓敏,张波,等.仙茅多糖对环磷酰胺所致免疫低下小鼠免疫功能的影响[J].中华中医药杂志,2016,31(12):5030-5034.
    [19]阿孜古丽·阿布都热合曼,韩玉明.肝癌患者血清Th1/Th2相关因子与免疫功能的相关性研究[J].国际检验医学杂志,2018,39(6):737-741.