33份薄壳山核桃品种种质资源的遗传多样性分析
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摘要
以阜阳市林业科学技术推广站苗圃的33份薄壳山核桃品种为试材,利用ISSR分子标记技术对其进行了遗传多样性分析。结果表明用8条引物对33份样品进行扩增,共检测扩增位点58个,其中多态性位点45个,多态性位点百分率(PPB)为77.59%,Nei’s基因多样性(H)为0.301,Shannon信息指数(I)为0.418,各品种间UPGMA遗传距离在0.67～0.84之间。ISSR可以有效揭示薄壳山核桃品种间的遗传多样性,研究结果对于薄壳山核桃品种鉴定和杂交育种具有重要参考价值。
Taking 33 pecan varieties in nursery garden of Fuyang Forestry Science and Technology Extension Station as materials, the genetic diversity of them was analyzed by ISSR molecular marker technology. The results showed that 58 bands were amplified by 8 informative and reliable primers, of which 45 were polymorphic loci. The percentage of polymorphic loci(PPB)was 77.59%, Nei's gene diversity(H) was equaled to 0.301, Shannon's Information index(I) was 0.418. The genetic distance of UPGMA between varieties was between 0.67 and 0.84. ISSR can effectively reveal the genetic diversity among pecan varieties,and the research results have important reference value for the evolution and classification of varieties.
Taking 33 pecan varieties in nursery garden of Fuyang Forestry Science and Technology Extension Station as materials, the genetic diversity of them was analyzed by ISSR molecular marker technology. The results showed that 58 bands were amplified by 8 informative and reliable primers, of which 45 were polymorphic loci. The percentage of polymorphic loci(PPB)was 77.59%, Nei's gene diversity(H) was equaled to 0.301, Shannon's Information index(I) was 0.418. The genetic distance of UPGMA between varieties was between 0.67 and 0.84. ISSR can effectively reveal the genetic diversity among pecan varieties,and the research results have important reference value for the evolution and classification of varieties.
引文
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[6]李金博,高丽华,周美亮,等.15个白三叶品种的ISSR和ITS遗传多样性分析[J].草业科学,2016,33(6):1147-1153.
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[19]林二培,马海泉,樊民亮,等.百山祖冷杉SSR体系的建立及人工辅助授粉子代的初步鉴定[J].浙江农林大学学报,2011,28(2):234-240.
[20]ROGER G W.Pecan production in the southeast a guide for growers[M].Alabama:Alabama Operative Extension Ser_vice,Auburn University,1989.
[21]范海燕,范可章,蔡健,等.小麦K-CMS保持系品种遗传多样性的系统聚类分析[J].阜阳师范学院学报(自然科学版),2015,32(4):69-73.
[2]董凤祥,王贵禧.薄壳山核桃引种及栽培技术[M].北京:金盾出版社,2003.
[3]张日清,陈建华,夏传格,等.我国引种美国山核桃科学研究进展[J].湖南林业科技,2001,28(2):6-9.
[4]彭方仁.美国薄壳山核桃产业发展现状及对我国的启示[J].林业科技开发,2014,28(6):1-5.
[5]李超,罗淑萍,曾斌,等.新疆核桃种质资源遗传多样性的ISSR分析[J].中国农业科学,2011,44(9):1871-1879.
[6]李金博,高丽华,周美亮,等.15个白三叶品种的ISSR和ITS遗传多样性分析[J].草业科学,2016,33(6):1147-1153.
[7]翟梅枝,肖志娟,许静,等.ISSR和SSR体系优化及在分析不同核桃品种遗传多样性上的应用[J].东北农业大学学报,2014,45(1):90-97.
[8]周亚星,周伟.ISSR分子标记技术在作物遗传育种中的应用[J].内蒙古民族大学学报(自然科学版),2011,26(6):682-684.
[9]GRAUKE L J,IQBAL M J,REDDY A S,et al.Developing microsatellite DNA markers in pecan[J].Amer Soc Hort Sci,2003,128(3):374-380.
[10]李晖,张瑞,彭方仁,等.美国山核桃种质资源遗传多样性ISSR分析[J].南京林业大学学报(自然科学版),2015,39(4):7-12.
[11]贾展慧,宣继萍,张计育,等.薄壳山核桃转录组中的SSR位点信息分析[R].分子植物育种,2018-9-15,http://kns.cnki.net/kcms/detail/46.1068.20180913.1428.024.html
[12]CONNER P J,WOOD B W.Identification of pecan cultivars and their genetic relatedness as determined by randomly amplied polymorphic DNA analysis[J].JAm SocHortic Sci,2001,126:474-480.
[13]邹喻苹,葛颂,王晓东.系统与进化植物学中的分子标记[M].北京:科学出版社,2001:16-17.
[14]欧阳彩虹,何桥,秦国新,等.25个菊花品种遗传多样性的ISSR分析[J].山西农业大学学报(自然科学版),2010,30(3):201-204
[15]张日清,何方,吕德芳,等.美国山核桃群体遗传多样性的RAPD分析[J].经济林研究,2001,19(2):1-6.
[16]YEH F C,YANG R C,BOYLE T B J,et al.POP-GENE:the user friendly shareware for population genetic analysis(Ver.1.32)[M].Canada:Moleeular Biology and Biotechnology Centre,University of Albreta,1997.(program available from:http://www.ualbreta.ca/~fyeh/).
[17]ROHLFF J.NTSYS-pc2.0 Numerical Taxonomy and Multivariate Analysis System.Exeter Software[CP/DK].New York,1998.
[18]WEN M F,WANG H Y,XIA Z Q,et al.Development of EST-SSR and genomic-SSR markers to assess genetic diversity in Jatropha curcas L[J].BMC Research Notes,2010,3:42.
[19]林二培,马海泉,樊民亮,等.百山祖冷杉SSR体系的建立及人工辅助授粉子代的初步鉴定[J].浙江农林大学学报,2011,28(2):234-240.
[20]ROGER G W.Pecan production in the southeast a guide for growers[M].Alabama:Alabama Operative Extension Ser_vice,Auburn University,1989.
[21]范海燕,范可章,蔡健,等.小麦K-CMS保持系品种遗传多样性的系统聚类分析[J].阜阳师范学院学报(自然科学版),2015,32(4):69-73.
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