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欧前胡素逆转A2780/Taxol多药耐药作用及其机制研究
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  • 英文篇名:Imperatorin reverses multidrug resistance of Huma Oophoroma Cancer Cell A2780/Taxol and its mechanism
  • 作者:李志勇 ; 汤涛 ; 梁新丽 ; 祝婧云
  • 英文作者:Li Zhiyong;Tang Tao;Liang Xinli;Zhu Jingyun;Department of pharmacy,the Fourth Affiliated Hospital of Nanchang University;Key Laboratory of Modern Preparation of Traditional Chineses Medicine,Ministry of Education,Jiangxi University of Traditional Chinese Medicine;
  • 关键词:欧前胡素 ; 人卵巢癌细胞株 ; 耐药性 ; 逆转作用
  • 英文关键词:Imperatorin;;Huma Oophoroma cancer cell;;drug resistance;;reverse effect
  • 中文刊名:中药药理与临床
  • 英文刊名:Pharmacology and Clinics of Chinese Materia Medica
  • 机构:南昌大学第四附属医院药学部;江西中医药大学现代中药制剂教育部重点实验室;
  • 出版日期:2019-04-15
  • 出版单位:中药药理与临床
  • 年:2019
  • 期:02
  • 基金:江西省自然科学基金项目(20161ACB21020)
  • 语种:中文;
  • 页:33-36
  • 页数:4
  • CN:51-1188/R
  • ISSN:1001-859X
  • 分类号:R285.5
摘要
目的:探讨欧前胡素对人卵巢癌耐紫杉醇细胞株A2780/Taxol耐药性的逆转作用及其可能的作用机制。方法:采用四甲基偶氮唑盐(MTT)比色法检测欧前胡素对人卵巢癌细胞A2780和人卵巢癌耐紫杉醇细胞株A2780/Taxol增殖的影响;观察欧前胡素作用A2780/Taxol细胞24h之后对紫杉醇耐药性的逆转效果;采用高效液相-荧光检测法和Western blot分别对其机制进行研究。结果:A2780/Taxol对紫杉醇耐药倍数为4.34(1.5        Objective: To study the reverse effect and mechanism of imperatorin on the drug resistance of A2780/Taxol cells. Methods: MTT was used to study its effect on proliferation of A2780 and A2780/Taxol cell lines. Drug resistance of A2780/Taxol cell lines against paclitaxel was observed after treated by imperatorin for 24 hours. HPLC-FLD and western blot were used to study its mechanism. Results: The results showed that the multidrug resistance of A2780/Taxol against paclitaxel was 4.34 times(1.5
引文
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