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Elsevier电子期刊(14)

在“Elsevier电子期刊”中，命中：14条，耗时：0.0529521 秒

在所有数据库中总计命中：18条

1.High-level expression of prolyl endopeptidase in Pichia pastoris using PLA₂ as a fusion partner

作者：Ting Jiang^a ; ^{jiangting75@outlook.com" class="auth_mail" title="E-mail the corresponding author} ; Chao Kang^a ; ^b ; Xiao-Wei Yu^a ; ^{yuxw@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Yan Xu^a ; ^{yxu@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author}

关键词：PEP ; prolyl endopeptidase ; MOH ; recombinant PEP ; PLA2 ; phospholipase A2 ; CBD ; cellulose-binding domain ; SUMO ; small ubiquitin-related modifier ; MBP ; maltose binding protein ; GST ; glutathione-S-transferase ; BMGY ; buffered minimal glycerol-complex medium ; BMMY ; buffered minimal methanol-complex medium ; LB ; luria-bertani ; LLB ; low salt luria-bertani ; CLMH ; CBD fused with MOH ; SLMH ; SUMO fused with MOH ; MLMH ; MBP fused with MOH ; PLMH ; PLA2 fused with MOH ; PMF ; peptide mass fingerprinting ; DTT ; dithi

刊名：Journal of Molecular Catalysis B: Enzymatic

出版年：2016

2.Expression and biochemical characterization of recombinant α-l-rhamnosidase r-Rha1 from Aspergillus niger JMU-TS528

作者：Lijun Li^a ; ^b ; ^c ; Yue Yu^a ; Xia Zhang^a ; Zedong Jiang^a ; ^b ; ^c ; Yanbing Zhu^a ; ^b ; ^c ; Anfeng Xiao^a ; ^b ; ^c ; Hui Ni^a ; ^b ; ^c ; ^{nihui@jmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Feng Chen^a ; ^d

关键词：PCR ; polymerase chain reaction ; cDNA ; complementary deoxyribonucleic acid ; RT-PCR ; reverse transcription-polymerase chain reaction ; BMGY ; buffered glycerol-complex medium ; BMMY ; buffered methanol-complex medium ; YNB ; yeast nitrogen base ; SDS ; sodium dodecyl sulphate ; PAGE ; polyacrylamide gel electrophoresis ; CBB ; coomassie brilliant blue ; MALDI-TOF ; matrix-assisted laser desorption/ionization time of flight ; MS ; mass spectrometry ; HPLC ; high performance liquid chromatography ; pNPR ; 4-nitrophenyl

刊名：International Journal of Biological Macromolecules

出版年：2016

3.Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring

作者：Alexander Templar ; Stefan Woodhouse ; Eli Keshavarz-Moore ; Darren N. Nesbeth ; ^{d.nesbeth@ucl.ac.uk" class="auth_mail" title="E-mail the corresponding author}

关键词：PCR ; polymerase chain reaction ; BMGY ; buffered glycerol complex medium ; e-pPCR ; end-point PCR ; HCD ; high cell density ; qPCR ; quantitative PCR ; YPD ; yeast extract peptone dextrose ; SF ; shake flask ; wcw ; wet cell weight ; WCB ; working cell bank ; LRE ; linear regression of efficiency ; OCF ; optical calibration factor

刊名：Journal of Microbiological Methods

出版年：2016

4.Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein

作者：Gui-Hong Cha¹ ; Yuan Liu¹ ; Ting Peng ; Ming-Zhu Huang ; Chen-Ying Xie ; Yu-Chao Xiao ; Wei-Na Wang ; ^{wangwn@scnu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{weina63@aliyun.com" class="auth_mail" title="E-mail the corresponding author}

关键词：P. pastoris ; Pichia pastoris ; L. vannamei (Lv) ; Litopenaeus vannamei ; V. alginolyticus ; Vibrio alginolyticus ; B. subtilis ; Bacillus subtilis ; S. aureus ; Staphylococcus aureus ; A. veronii ; Aeromonas veronii ; BSA ; bovine serum albumin ; YPD ; yeast extract peptone dextrose medium ; BMGY ; buffered minimal glycerol-complex medium ; BMMY ; buffered minimal methanol-complex medium ; YPDS ; yeast extract peptone dextrose sorbitol medium ; TBST ; Tris-buffered saline with 0.05% Tween 20 ; PVDF ; polyvinylidene dif

刊名：Molecular Immunology

出版年：2015

5.Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter

作者：Mariafrancesca Scalise^a ; Lorena Pochini^a ; Piero Pingitore^a ; Kristina Hedfalk^b ; Cesare Indiveri^a ; ^{cesare.indiveri@unical.it" class="auth_mail" title="E-mail the corresponding author}

关键词：C12E8 ; octaethylene glycol monododecyl ether ; MeAIB ; (α-methylamino)isobutyric acid ; BCH ; 2-aminobicyclo-(22 ; 1)-heptane-2-carboxylic acid ; YPDS ; yeast extract peptone dextrose sorbitol ; BMGY ; buffered glycerol-complex medium ; DOC ; Na-deoxycholate ; NP-40 ; Nonidet ; NAC ; N-acetyl cysteine ; CMC ; carbossi methyl cysteine

刊名：FEBS Letters

出版年：2015

6.Cloning, expression and purification of squalene synthase from Candida tropicalis in Pichia pastoris

作者：Pey Yee Lee ; Voon Chen Yong ; Rozita Rosli ; Lay Harn Gam ; Pei Pei Chong

关键词：AOX1 ; alcohol oxidase 1 ; bp ; base pair ; BSA ; bovine serum albumin ; BMGY ; buffered glycerol-complex medium ; BMMY ; buffered methanol-complex medium ; DTT ; dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; LiCl ; lithium chloride ; PBS ; phosphate-buffered saline ; TBST ; Tris-buffered saline with 0.05% Tween 20 ; PMSF ; phenylmethylsulfonyl fluoride ; PVDF ; polyvinylidene difluoride ; SS ; squalene synthase ; YPD ; yeast extract-peptone-dextrose

刊名：Protein Expression and Purification

出版年：February, 2014

7.Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of function

作者：Piero Pingitore^a ; ^b ; ¹ ; Carlo Pirazzi^c ; ¹ ; Rosellina M. Mancina^c ; ^d ; Benedetta M. Motta^c ; ^e ; Cesare Indiveri^a ; Arturo Pujia^d ; Tiziana Montalcini^d ; Kristina Hedfalk^b ; ^{kristina.hedfalk@chem.gu.se" class="auth_mail} ; Stefano Romeo^c ; ^d ; ^{stefano.romeo@wlab.gu.se" class="auth_mail}

关键词：PNPLA3 ; patatin-like phospholipase domain containing 3 ; ADPN ; adiponutrin ; Sf9 ; Spodoptera frugiperda 9 ; LPAAT ; lysophosphatidic acid acyl transferase ; YPDS ; Yeast Extract Peptone Dextrose plus Sorbitol ; BMGY ; buffered glycerol-complex medium ; BMMY ; buffered methanol-complex medium ; CRB ; cell resuspension buffer ; TCA ; trichloroacetic acid ; hAQP1 ; human Aquaporin 1 ; IFV ; initial fermentation volume ; Ni&ndash ; NTA ; nickel&ndash ; nitrilotriacetic acid ; BSA ; bovine serum albumin ; DTT ; dithiothreitol

刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids

出版年：April, 2014

8.The effect of ¦Á-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris

作者：Geoff P. Lin-Cereghino^a ; Carolyn M. Stark^a ; Daniel Kim^a ; Jennifer Chang^a ; Nadia Shaheen^a ; Hansel Poerwanto^a ; Kimiko Agari^a ; Pachai Moua^a ; Lauren K. Low^a ; Namphuong Tran^a ; Amy D. Huang^a ; Maria Nattestad^a ; Kristin T. Oshiro^a ; John William Chang^a ; Archana Chavan^b ; Jerry W. Tsai^b ; Joan Lin-Cereghino^a ; ^{jlincere@pacific.edu} ;

关键词：Ala ; alanine ; AP ; alkaline phosphatase ; BMGY ; buffered minimal glycerol-complex medium ; BMMY ; buffered minimal methanol-complex medium ; CalB ; Candida antarctica lipase B ; DMSO ; dimethyl sulfoxide ; dNTP ; deoxyribonucleoside triphosphate ; ER ; endoplasmic re

刊名：Gene

出版年：2013

9.Characterisation of a thermo-alkali-stable lipase from oil-contaminated soil using a metagenomic approach

作者：Jianhua Zheng^a ; Cuina Liu^b ; Liguo Liu^a ; Qi Jin^a ; ^{zdsys@vip.sina.com}

关键词：BMGY ; buffered glycerol-complex medium ; BMMY ; buffered methanol-complex medium ; dNTP ; deoxynucleoside triphosphate ; DTT ; 1 ; 4-dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; FAMEs ; fatty acid methyl esters ; LAOR ; LB medium supplemented with ampicill

刊名：Systematic and Applied Microbiology

出版年：2013

10.Improvement of recombinant endoglucanase produced in Pichia pastoris KM71 through the use of synthetic medium for inoculum and pH control of proteolysis

作者：Theppanya Charoenrat¹ ; Nitisarn Khumruaengsri¹ ; Peerada Promdonkoy² ; Nakul Rattanaphan² ; Lily Eurwilaichitr² ; Sutipa Tanapongpipat² ; Niran Roongsawang² ; ^{niran.roo@biotec.or.th}

关键词：Inoculum development ; Lag time ; Pichia pastoris ; Endoglucanase ; Proteolysis

刊名：Journal of Bioscience and Bioengineering

出版年：2013

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