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Elsevier电子期刊(30)

在“Elsevier电子期刊”中，命中：30条，耗时：0.0129923 秒

在所有数据库中总计命中：30条

1.Expression and biochemical characterization of recombinant α-l-rhamnosidase r-Rha1 from Aspergillus niger JMU-TS528

作者：Lijun Li^a ; ^b ; ^c ; Yue Yu^a ; Xia Zhang^a ; Zedong Jiang^a ; ^b ; ^c ; Yanbing Zhu^a ; ^b ; ^c ; Anfeng Xiao^a ; ^b ; ^c ; Hui Ni^a ; ^b ; ^c ; ^{nihui@jmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Feng Chen^a ; ^d

关键词：PCR ; polymerase chain reaction ; cDNA ; complementary deoxyribonucleic acid ; RT-PCR ; reverse transcription-polymerase chain reaction ; BMGY ; buffered glycerol-complex medium ; BMMY ; buffered methanol-complex medium ; YNB ; yeast nitrogen base ; SDS ; sodium dodecyl sulphate ; PAGE ; polyacrylamide gel electrophoresis ; CBB ; coomassie brilliant blue ; MALDI-TOF ; matrix-assisted laser desorption/ionization time of flight ; MS ; mass spectrometry ; HPLC ; high performance liquid chromatography ; pNPR ; 4-nitrophenyl

刊名：International Journal of Biological Macromolecules

出版年：2016

2.Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris

作者：Florian Wolfgang Krainer^a ; ^{florian.krainer@tugraz.at" class="auth_mail" title="E-mail the corresponding author} ; Barbara Darnhofer^b ; ^c ; ^d ; Ruth Birner-Gruenberger^b ; ^c ; ^d ; Anton Glieder^a

关键词：ABTS ; 2 ; 2&prime ; -azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt ; BSM ; basal salts medium ; BMGY ; buffered minimal glycerol yeast medium ; BSA ; bovine serum albumin ; Ca-TBS ; calcium-supplemented tris&ndash ; buffered saline ; DCW ; dry cell weight ; ELISA ; enzyme-linked immunosorbent assays ; HRP ; horseradish peroxidase ; IgG ; immunoglobulin G ; PTM1 ; trace element solution ; SpG ; streptococcal protein G ; TMB ; 3 ; 3&prime5 ; 5&prime ; -tetramethylbenzidine dihydrochloride hydrate ; YE ; Bacto&tr

刊名：Journal of Biotechnology

出版年：2016

3.High-level expression of prolyl endopeptidase in Pichia pastoris using PLA₂ as a fusion partner

作者：Ting Jiang^a ; ^{jiangting75@outlook.com" class="auth_mail" title="E-mail the corresponding author} ; Chao Kang^a ; ^b ; Xiao-Wei Yu^a ; ^{yuxw@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Yan Xu^a ; ^{yxu@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author}

关键词：PEP ; prolyl endopeptidase ; MOH ; recombinant PEP ; PLA2 ; phospholipase A2 ; CBD ; cellulose-binding domain ; SUMO ; small ubiquitin-related modifier ; MBP ; maltose binding protein ; GST ; glutathione-S-transferase ; BMGY ; buffered minimal glycerol-complex medium ; BMMY ; buffered minimal methanol-complex medium ; LB ; luria-bertani ; LLB ; low salt luria-bertani ; CLMH ; CBD fused with MOH ; SLMH ; SUMO fused with MOH ; MLMH ; MBP fused with MOH ; PLMH ; PLA2 fused with MOH ; PMF ; peptide mass fingerprinting ; DTT ; dithi

刊名：Journal of Molecular Catalysis B: Enzymatic

出版年：2016

4.Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring

作者：Alexander Templar ; Stefan Woodhouse ; Eli Keshavarz-Moore ; Darren N. Nesbeth ; ^{d.nesbeth@ucl.ac.uk" class="auth_mail" title="E-mail the corresponding author}

关键词：PCR ; polymerase chain reaction ; BMGY ; buffered glycerol complex medium ; e-pPCR ; end-point PCR ; HCD ; high cell density ; qPCR ; quantitative PCR ; YPD ; yeast extract peptone dextrose ; SF ; shake flask ; wcw ; wet cell weight ; WCB ; working cell bank ; LRE ; linear regression of efficiency ; OCF ; optical calibration factor

刊名：Journal of Microbiological Methods

出版年：2016

5.Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris

作者：Florian W. Krainer^a ; ^{flokrai@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Michaela A. Gerstmann^a ; ^{michaela.gerstmann@gmx.net" class="auth_mail" title="E-mail the corresponding author} ; Barbara Darnhofer^b ; ^c ; ^d ; ^{Barbara.Darnhofer@klinikum-graz.at" class="auth_mail" title="E-mail the corresponding author} ; Ruth Birner-Gruenberger^b ; ^c ; ^d ; ^{ruth.birner-gruenberger@medunigraz.at" class="auth_mail" title="E-mail the corresponding author} ; Anton Glieder^a ; ^{a.glieder@tugraz.at" class="auth_mail" title="E-mail the corresponding author}

关键词：ABTS ; 2 ; 2&prime ; azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt ; BSM ; basal salts medium ; BMD1% ; buffered minimal dextrose medium ; BMG1% ; buffered minimal glycerol medium ; BMGY ; buffered minimal glycerol yeast medium ; BMM ; buffered minimal methanol medium ; Ca-TBS ; calcium-supplemented tris-buffered saline ; DWP ; 96-deep well plate ; GRAS ; generally recognized as safe ; HRP ; horseradish peroxidase ; PTM1 ; trace element solution ; Rz ; Reinheitszahl ; UPR ; unfolded protein response ; YE

刊名：Journal of Biotechnology

出版年：2016

6.Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein

作者：Gui-Hong Cha¹ ; Yuan Liu¹ ; Ting Peng ; Ming-Zhu Huang ; Chen-Ying Xie ; Yu-Chao Xiao ; Wei-Na Wang ; ^{wangwn@scnu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{weina63@aliyun.com" class="auth_mail" title="E-mail the corresponding author}

关键词：P. pastoris ; Pichia pastoris ; L. vannamei (Lv) ; Litopenaeus vannamei ; V. alginolyticus ; Vibrio alginolyticus ; B. subtilis ; Bacillus subtilis ; S. aureus ; Staphylococcus aureus ; A. veronii ; Aeromonas veronii ; BSA ; bovine serum albumin ; YPD ; yeast extract peptone dextrose medium ; BMGY ; buffered minimal glycerol-complex medium ; BMMY ; buffered minimal methanol-complex medium ; YPDS ; yeast extract peptone dextrose sorbitol medium ; TBST ; Tris-buffered saline with 0.05% Tween 20 ; PVDF ; polyvinylidene dif

刊名：Molecular Immunology

出版年：2015

7.Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter

作者：Mariafrancesca Scalise^a ; Lorena Pochini^a ; Piero Pingitore^a ; Kristina Hedfalk^b ; Cesare Indiveri^a ; ^{cesare.indiveri@unical.it" class="auth_mail" title="E-mail the corresponding author}

关键词：C12E8 ; octaethylene glycol monododecyl ether ; MeAIB ; (α-methylamino)isobutyric acid ; BCH ; 2-aminobicyclo-(22 ; 1)-heptane-2-carboxylic acid ; YPDS ; yeast extract peptone dextrose sorbitol ; BMGY ; buffered glycerol-complex medium ; DOC ; Na-deoxycholate ; NP-40 ; Nonidet ; NAC ; N-acetyl cysteine ; CMC ; carbossi methyl cysteine

刊名：FEBS Letters

出版年：2015

8.Microstructure and properties of the low-power-laser clad coatings on magnesium alloy with different amount of rare earth addition

作者：Rundong Zhu ; Zhiyong Li ; ^{lizhiyong@nuc.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{lzhy_2002@126.com" class="auth_mail" title="E-mail the corresponding author} ; Xiaoxi Li ; Qi Sun

关键词：Laser clad ; AZ91D magnesium alloy ; Rare earth ; Microstructure ; Properties

刊名：Applied Surface Science

出版年：2015

9.Production optimization and expression of pectin releasing enzyme from Aspergillus oryzae PO

作者：Jia Chen ; Rujian Yang ; Min Chen ; Shaohua Wang ; Peng Li ; Yuelan Xia ; Li Zhou ; Jingli Xie ; Dongzhi Wei

关键词：PPase ; protopectinase ; RSM ; response surface methodology ; BBD ; Box&ndash ; Behnken Design ; PGase ; polygalacturonase ; PGA ; polygalacturonic acid ; MD ; minimal dextrose medium ; BMGY ; buffered dextrose-complex medium ; BMMY ; buffered methanol-complex medium

刊名：Carbohydrate Polymers

出版年：30 January, 2014

10.Cloning, expression and purification of squalene synthase from Candida tropicalis in Pichia pastoris

作者：Pey Yee Lee ; Voon Chen Yong ; Rozita Rosli ; Lay Harn Gam ; Pei Pei Chong

关键词：AOX1 ; alcohol oxidase 1 ; bp ; base pair ; BSA ; bovine serum albumin ; BMGY ; buffered glycerol-complex medium ; BMMY ; buffered methanol-complex medium ; DTT ; dithiothreitol ; EDTA ; ethylenediaminetetraacetic acid ; LiCl ; lithium chloride ; PBS ; phosphate-buffered saline ; TBST ; Tris-buffered saline with 0.05% Tween 20 ; PMSF ; phenylmethylsulfonyl fluoride ; PVDF ; polyvinylidene difluoride ; SS ; squalene synthase ; YPD ; yeast extract-peptone-dextrose

刊名：Protein Expression and Purification

出版年：February, 2014

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